Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,268,573 T→C 6.3% N289D (AAC→GAC)  PP_1109 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,268,5730TC 6.3% 192.4 / 2.0 63N289D (AAC→GAC) PP_1109GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (29/30);  new base C (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

CAAAACGCGGGGACAGCGACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTCGGGTTGTGGAAG  >  NC_002947/1268428‑1268721
                                                                                                                                                 |                                                                                                                                                    
cAAAACGCGGGGACAGCGACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTcc                                                                                                                                                  <  1:333285/150‑1 (MQ=255)
            aCAGCGACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAg                                                                                                                                      <  1:787974/150‑1 (MQ=255)
            aCAGCGACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAg                                                                                                                                      <  2:1397259/150‑1 (MQ=255)
                cGACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCt                                                                                                                                  >  1:127579/1‑150 (MQ=255)
                 gACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTc                                                                                                                                                   <  1:334852/132‑1 (MQ=255)
                 gACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTc                                                                                                                                                   >  2:334852/1‑132 (MQ=255)
                   cGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGgc                                                                                                                               <  1:1470324/150‑1 (MQ=255)
                      aGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGca                                                                                                                            >  1:1634202/1‑150 (MQ=255)
                             cagTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGTGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAgg                                                                                                                     <  2:672909/150‑1 (MQ=255)
                                      gCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTc                                                                                                            >  2:417767/1‑150 (MQ=255)
                                      gCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTc                                                                                                            >  1:1557719/1‑150 (MQ=255)
                                       cGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTcc                                                                                                           <  2:1634202/150‑1 (MQ=255)
                                         tgtgAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTcccc                                                                                                         <  1:290861/150‑1 (MQ=255)
                                                  cTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAAcgtc                                                                                                >  1:1215807/1‑150 (MQ=255)
                                                   tCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAg                                                                                                                                      <  2:936535/111‑1 (MQ=255)
                                                   tCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAg                                                                                                                                      >  1:936535/1‑111 (MQ=255)
                                                   tCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAAcgtcg                                                                                               <  2:1094106/150‑1 (MQ=255)
                                                      aTCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACCGACATGTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGTACAGCAGGGGTGAAGGCCCCATACACGTCGTcc                                                                                            >  2:246970/1‑150 (MQ=255)
                                                      aTCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTcc                                                                                            >  1:926973/1‑150 (MQ=255)
                                                         aGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCg                                                                                         <  2:1557719/150‑1 (MQ=255)
                                                           gTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCGATAAACGTCGTCCTCGAc                                                                                       >  2:95275/1‑150 (MQ=255)
                                                             cTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCa                                                                                     >  1:319107/1‑150 (MQ=255)
                                                                  gAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTCGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACgaag                                                                                >  2:907734/1‑150 (MQ=255)
                                                                        gCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCg                                                                          <  1:1520166/150‑1 (MQ=255)
                                                                        gCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCg                                                                          >  1:885616/1‑150 (MQ=255)
                                                                        gCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCg                                                                          >  2:1520166/1‑150 (MQ=255)
                                                                          aaTGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGaa                                                                                                               <  1:825044/111‑1 (MQ=255)
                                                                          aaTGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGaa                                                                                                               >  2:825044/1‑111 (MQ=255)
                                                                                   cACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCa                                                                                                        <  2:354791/109‑1 (MQ=255)
                                                                                   cACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCa                                                                                                        >  1:354791/1‑109 (MQ=255)
                                                                                        gCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGC                                                          <  2:1215807‑M1/150‑1 (MQ=255)
                                                                                           gCGAGGCCGACAGGCATCTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCA                                                       >  2:145375‑M1/1‑150 (MQ=255)
                                                                                              aGGCCGACAGTCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGa                                                    <  1:246970/150‑1 (MQ=255)
                                                                                                 ccGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCCGCGGTCTTCACCGCTGCGTGGATATCCGCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGgcgc                                                 >  1:1427265/1‑150 (MQ=255)
                                                                                                  cGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCa                                                             <  1:333926/137‑1 (MQ=255)
                                                                                                  cGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCa                                                             >  2:333926/1‑137 (MQ=255)
                                                                                                      aGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAg                                            >  2:1195927/1‑150 (MQ=255)
                                                                                                      aGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAg                                            <  2:319107/150‑1 (MQ=255)
                                                                                                         cATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCg                                         >  2:440957/1‑150 (MQ=255)
                                                                                                           tttGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTcc                                                                                                           <  2:527010/82‑1 (MQ=255)
                                                                                                           tttGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTcc                                                                                                           >  1:527010/1‑82 (MQ=255)
                                                                                                           tttGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGGTGAAGGCGtt                                       >  2:1494511/1‑150 (MQ=255)
                                                                                                           tttGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGtt                                       <  2:127579/150‑1 (MQ=255)
                                                                                                              ggAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGtg                                    <  1:772277‑M2/150‑3 (MQ=255)
                                                                                                               gagaAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGtgc                                   <  1:902125‑M2/150‑4 (MQ=255)
                                                                                                                     cTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTg                             <  2:772277/150‑1 (MQ=255)
                                                                                                                      tGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGa                            <  1:145375/150‑1 (MQ=255)
                                                                                                                           aTGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTtg                       <  1:842995/150‑1 (MQ=255)
                                                                                                                               aGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTtgctgc                   >  1:872221/1‑150 (MQ=255)
                                                                                                                                 aTAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCg                 <  2:885616/150‑1 (MQ=255)
                                                                                                                                 aTAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCg                 >  2:1628842/1‑150 (MQ=255)
                                                                                                                                 aTAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCg                 >  1:1013046/1‑150 (MQ=255)
                                                                                                                                 aTAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCg                 >  2:329116/1‑150 (MQ=255)
                                                                                                                                    aCCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTc              <  1:417767/150‑1 (MQ=255)
                                                                                                                                     cccGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGACGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTCg             >  2:637081/1‑150 (MQ=255)
                                                                                                                                      ccGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTtgct                     >  2:612089/1‑141 (MQ=255)
                                                                                                                                      ccGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTtgct                     <  1:612089/141‑1 (MQ=255)
                                                                                                                                      ccGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCCGTCgg            >  2:932324/1‑150 (MQ=255)
                                                                                                                                      ccGGTCAAGGTCGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTtgctgc                   <  1:1483592/143‑1 (MQ=255)
                                                                                                                                      ccGGTCAAGGTCGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTtgctgc                   >  2:1483592/1‑143 (MQ=255)
                                                                                                                                       cGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTCggg           <  2:1427265/150‑1 (MQ=255)
                                                                                                                                         gTCAAGGTCGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTCGGGtt         <  1:907734/150‑1 (MQ=255)
                                                                                                                                          tCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTCGGGTtg        <  2:926973/150‑1 (MQ=255)
                                                                                                                                                ttGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTCGGGTTGTGGAAg  <  1:329116/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAAAACGCGGGGACAGCGACGCAGGTCAGCAGTTTAAGGCGTGTGAGCGGCTCGATCAGGTCTTGCGAGCAGGCAATGAACCCCACGCGCAGCGAGGCCGACAGGCATTTGGAGAAGCTGCCGATGTAGATAACCCGGTCAAGGTTGTCCAGCTCGGCAAAGGTCTGGCGCACAGCAGGGCTGAAGTCCCCATAAACGTCGTCCTCGACCACGAAGAACTCGTGCTCCACCGCCAGGCGCAGGACGCTGAAGGCGTTGTGCGGGCTGATGTTGCTGCCGGTCGGGTTGTGGAAG  >  NC_002947/1268428‑1268721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑