Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,307,455 T→G 10.4% N57H (AAC→CAC)  livK ← branched‑chain amino acids ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,307,4550TG10.4% 122.0 / 6.0 48N57H (AAC→CAC) livKbranched‑chain amino acids ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (17/26);  new base G (2/3);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

CTGGGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTGCGGCGAAC  >  NC_002947/1307306‑1307603
                                                                                                                                                     |                                                                                                                                                    
ctggGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGtt                                                                                                                                                      <  2:788437/150‑1 (MQ=255)
   ggTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGat                                                                                                                                                   <  2:1306820/150‑1 (MQ=255)
       gagctggagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATcc                                                                                                                                               <  1:1412729/150‑1 (MQ=255)
         gctggagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCtt                                                                                                                                             <  1:655929/150‑1 (MQ=255)
                     gTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGgcttgctt                                                                                                                                 <  1:791899/150‑1 (MQ=255)
                        gCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGgc                                                                                                                              <  2:852928/150‑1 (MQ=255)
                           gACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGgc                                                                                                                              <  1:1099219/147‑1 (MQ=255)
                           gACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGgc                                                                                                                              >  2:1099219/1‑147 (MQ=255)
                           gACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGcc                                                                                                                           >  2:86971/1‑150 (MQ=255)
                              cACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGat                                                                                                                                                   <  1:53606/123‑1 (MQ=255)
                              cACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGat                                                                                                                                                   >  2:53606/1‑123 (MQ=255)
                                 gAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGaa                                                                                                                     <  1:187940/150‑1 (MQ=255)
                                  aaCTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAAc                                                                                                                    <  1:135243/150‑1 (MQ=255)
                                       gACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGatgg                                                                                                                                                 <  2:73461/116‑3 (MQ=255)
                                       gACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGatgg                                                                                                                                                 >  1:73461/1‑114 (MQ=255)
                                          gCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTc                                                                                                            >  2:592741/1‑150 (MQ=255)
                                                  tGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACt                                                                                                    <  1:592741/150‑1 (MQ=255)
                                                   gACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGtt                                                                                                                                                      >  2:80939/1‑99 (MQ=255)
                                                   gACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGtt                                                                                                                                                      <  1:80939/99‑1 (MQ=255)
                                                   gACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTg                                                                                                   <  1:1060139/150‑1 (MQ=255)
                                                   gACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTg                                                                                                   >  2:1092801/1‑150 (MQ=255)
                                                       aCTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCAACGCCGCGGGCGGCGTGGa                                                                                                                                                    <  2:214203/97‑1 (MQ=255)
                                                           tgttgGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACgg                                                                                           <  2:825885/150‑1 (MQ=255)
                                                                             ccTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCgg                                                                         >  2:1299600/1‑150 (MQ=255)
                                                                                gTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGa                                                                      <  1:1221098/150‑1 (MQ=255)
                                                                                 tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGAt                                                                     >  2:253138/1‑150 (MQ=255)
                                                                                 tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGAt                                                                     >  2:1543381/1‑150 (MQ=255)
                                                                                 tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGAt                                                                     <  1:816502/150‑1 (MQ=255)
                                                                                        tCGCAGGCGTCTTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATc                                                              >  2:2974/1‑150 (MQ=255)
                                                                                         cGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCt                                                             <  1:86971/150‑1 (MQ=255)
                                                                                            atgCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGCCGCCGTAGTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGa                                                          <  2:1192305/148‑1 (MQ=255)
                                                                                             ggCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGACGATCGGCGGTTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                                         <  2:222055/150‑1 (MQ=255)
                                                                                              gCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATg                                                        <  1:253138/150‑1 (MQ=255)
                                                                                                    tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcg                                                  >  2:610143/1‑150 (MQ=255)
                                                                                                      gtATTCCTTGGCTTAGGGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggc                                                >  2:1302655/1‑150 (MQ=255)
                                                                                                      gtATTACTTGGCTTCGAGCATATTGCGATCCACGGCTTCTGCGTCGTTGATAGCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCAGTACTGGGTCACTGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggc                                                >  2:1177454/1‑150 (MQ=255)
                                                                                                        aTTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcg                                               >  2:1156347/1‑149 (MQ=255)
                                                                                                        aTTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcg                                               <  1:1156347/149‑1 (MQ=255)
                                                                                                         ttCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCggcggc                                             >  2:1643239/1‑150 (MQ=255)
                                                                                                                gCTTCGAGCATCTTGCCACCGAGTCCGCCCCGGGCGTTGATATCCTTGATGGATTGCTTGGCGCCGATGAACTGCATGTCGCCTTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGaa                                      <  1:903642/150‑1 (MQ=255)
                                                                                                                 cTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACg                                                                                            <  2:1273669/95‑1 (MQ=255)
                                                                                                                 cTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACg                                                                                            >  1:1273669/1‑95 (MQ=255)
                                                                                                                     gAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggct                                 >  2:1540671/1‑150 (MQ=255)
                                                                                                                      aGCATCGTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctg                                <  2:1398074/150‑1 (MQ=255)
                                                                                                                       gCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctgg                               <  1:1299600/150‑1 (MQ=255)
                                                                                                                                 aTCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc                     <  1:2974/150‑1 (MQ=255)
                                                                                                                                  tCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGcc                    <  2:599952/150‑1 (MQ=255)
                                                                                                                                     aCGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGt                 >  1:384480/1‑150 (MQ=255)
                                                                                                                                        ccgcccgcGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctg                                <  1:1166313/132‑1 (MQ=255)
                                                                                                                                        ccgcccgcGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctg                                >  2:1166313/1‑132 (MQ=255)
                                                                                                                                               cggcgTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTgcgg       <  1:1643239/150‑1 (MQ=255)
                                                                                                                                                    ttGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTGCGGCGAAc  >  2:615895/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGGGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGCCAGTACCATTGCGGCGAAC  >  NC_002947/1307306‑1307603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑