| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,346 | T→G | 8.8% | T13P (ACG→CCG) | trmE ← | GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,346 | 0 | T | G | 8.8% | 150.7 / 8.0 | 57 | T13P (ACG→CCG) | trmE | GTPase |
| Reads supporting (aligned to +/- strand): ref base T (21/31); new base G (2/3); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
TCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGG > NC_002947/6198‑6490 | tCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTg < 2:1571008/150‑1 (MQ=255) gTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGc < 2:672023/150‑1 (MQ=255) gCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATgg > 1:1110368/1‑150 (MQ=255) cACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCAGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCC‑AGGGCCGGGGCGGTgtg < 2:85188/150‑3 (MQ=255) aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACATCGCCACGGCCCAGGGGCCGGGGCggtgtg < 2:660909/150‑6 (MQ=255) cATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGttt < 2:1110368/150‑1 (MQ=255) tcgCGGAATGGATCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGc > 2:828428/1‑150 (MQ=255) gTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt < 2:397872/144‑1 (MQ=255) gTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt > 1:397872/1‑144 (MQ=255) tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc < 1:1216973/150‑1 (MQ=255) tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc < 1:240670/150‑1 (MQ=255) gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc > 1:974361/1‑138 (MQ=255) gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc < 2:974361/138‑1 (MQ=255) gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGc > 1:649861/1‑150 (MQ=255) gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGc < 2:1554029/150‑1 (MQ=255) tGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACACCCTGACAAATACCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGTCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCctc < 1:524011/150‑1 (MQ=255) tgcgGGGTGAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc < 1:1096010/149‑1 (MQ=255) cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc < 2:1539124/150‑1 (MQ=255) cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc < 2:1205701/150‑1 (MQ=255) gcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACg > 2:1060617/1‑150 (MQ=255) gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc < 1:662671/150‑1 (MQ=255) aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc > 2:1153201/1‑150 (MQ=255) aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc > 2:1273055/1‑150 (MQ=255) aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc > 1:1173728/1‑150 (MQ=255) aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc > 1:335093/1‑150 (MQ=255) aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc > 1:996866/1‑150 (MQ=255) gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc < 2:591483/100‑1 (MQ=255) gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc > 1:591483/1‑100 (MQ=255) ttGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑gggg > 2:592273/1‑150 (MQ=255) gCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGt < 1:381709/150‑1 (MQ=255) gCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGtttt < 2:649861/150‑1 (MQ=255) agagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GTGGCGTTTTTTa < 1:1060617/150‑1 (MQ=255) agagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTa < 1:322473/150‑1 (MQ=255) gagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTAt < 1:1061592/150‑1 (MQ=255) cGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAg < 1:98430/150‑1 (MQ=255) ggACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑g < 1:49992/130‑1 (MQ=255) ggACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑g > 2:49992/1‑130 (MQ=255) aCCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGc < 2:996866/150‑1 (MQ=255) tGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTa < 2:1173728/150‑1 (MQ=255) gACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGcaca > 1:723282/1‑61 (MQ=255) gACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGcaca < 2:723282/61‑1 (MQ=255) gACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAt > 2:243027/1‑150 (MQ=255) cAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTa < 1:828428/150‑1 (MQ=255) cAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTa < 1:1592469/150‑1 (MQ=255) aTTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc < 1:592273/150‑1 (MQ=255) cacaCCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc < 1:243027/150‑1 (MQ=255) acacCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCa > 1:123075/1‑150 (MQ=255) atcGCCACGGCCC‑AGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag > 2:575248/3‑150 (MQ=255) atcGCCACGGCCC‑AGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTa < 2:84631/119‑1 (MQ=255) atcGCCACGGCCC‑AGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGACGGTTTTTa > 1:84631/3‑121 (MQ=255) ccGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGc < 2:1187961/66‑1 (MQ=255) ccGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGc > 1:1187961/1‑66 (MQ=255) ccGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagcag > 1:428037/1‑150 (MQ=255) cc‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCtttt > 1:841760/1‑150 (MQ=255) tGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCtttttt < 1:204140/150‑1 (MQ=255) tGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCtttttt < 2:335093/150‑1 (MQ=255) ggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTgg > 2:417057/1‑150 (MQ=255) | TCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCC‑TGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGG > NC_002947/6198‑6490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |