Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,940 A→C 10.2% H61P (CAC→CCC)  PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,9400AC10.2% 172.1 / 9.0 63H61P (CAC→CCC) PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base A (31/25);  new base C (3/4);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAACCG  >  NC_002947/1416796‑1417089
                                                                                                                                                |                                                                                                                                                     
aCGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGc                                                                                                                                                  >  1:826003/1‑150 (MQ=255)
      cGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATgggg                                                                                                                                            <  1:186063/150‑1 (MQ=255)
      cGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGAGTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCGCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATgggg                                                                                                                                            <  2:143039/150‑1 (MQ=255)
           aTCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGc                                                                                                                                       <  1:1491672/150‑1 (MQ=255)
           aTCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGc                                                                                                                                       <  1:1246083/150‑1 (MQ=255)
              gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCGGcat                                                                                                                                     <  1:1031037/149‑3 (MQ=255)
              gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCGGcat                                                                                                                                     >  2:1031037/1‑147 (MQ=255)
                     gtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCAc                                                                                                                             <  2:826003/150‑1 (MQ=255)
                        acacGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATgggg                                                                                                                                            >  2:756770/1‑132 (MQ=255)
                        acacGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATgggg                                                                                                                                            <  1:756770/132‑1 (MQ=255)
                        acacGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgc                                                                                                                          <  1:1166670/150‑1 (MQ=255)
                        acacGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgc                                                                                                                          >  1:922963/1‑150 (MQ=255)
                          acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgcgc                                                                                                                        >  2:1469500/1‑150 (MQ=255)
                            gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                                                                                      >  2:141514/1‑150 (MQ=255)
                                   ctagTCGATTGCGGCTTTTCCCTGCGTGAAACCGCGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGTTAACCCACGATCGTGCCGACCCTGTGCATGCGTTCGGCTTGCTGTCACGGCTCTACAATGTa                                                                                                               <  2:639248/147‑1 (MQ=255)
                                     ggTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAcc                                                                                                             >  1:317172/1‑150 (MQ=255)
                                                gCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                                  >  1:1544929/1‑150 (MQ=255)
                                                       ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                                                                                           >  2:914974/1‑150 (MQ=255)
                                                             tGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGc                                                                                                >  2:39662/1‑139 (MQ=255)
                                                             tGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGc                                                                                                <  1:39662/139‑1 (MQ=255)
                                                                aaCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg                                                                                  <  1:562833/150‑1 (MQ=255)
                                                                 aCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCGCGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcgc                                                                                 >  1:367426/1‑150 (MQ=255)
                                                                                   ctgctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCt                                                                                                                                  <  2:981326/83‑1 (MQ=255)
                                                                                   ctgctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCt                                                                                                                                  >  1:981326/1‑83 (MQ=255)
                                                                                      ctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGACACTTTGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAg                                                            <  2:1379429/150‑1 (MQ=255)
                                                                                             tttCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAGCATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCg                                                     >  1:162897/1‑150 (MQ=255)
                                                                                              ttCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCgg                                                    <  1:1138899/150‑1 (MQ=255)
                                                                                                   cagctcagctCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTtc                                               >  1:1644393/1‑150 (MQ=255)
                                                                                                   cagctcagctCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTtc                                               <  1:1469500/150‑1 (MQ=255)
                                                                                                     gctcagctcaGTGCAGTGCTGGTCACCCACGAGCATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTtctc                                             <  2:162897/150‑1 (MQ=255)
                                                                                                         agctcagTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCgt                                         >  1:428145/1‑150 (MQ=255)
                                                                                                           ctcagTGCAGTGCTTGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCgtgt                                       >  2:242062/1‑150 (MQ=255)
                                                                                                             cagtgcagtgCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTgg                                     <  2:1544929/150‑1 (MQ=255)
                                                                                                              agtgcagtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCt                                                                                                                                  <  1:1272592/56‑1 (MQ=255)
                                                                                                              agtgcagtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCt                                                                                                                                  >  2:1272592/1‑56 (MQ=255)
                                                                                                                  cagtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCaaa                                >  1:1078330/1‑150 (MQ=255)
                                                                                                                   agtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAg                               >  2:1338829/1‑150 (MQ=255)
                                                                                                                      gcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCt                            <  1:1338829/150‑1 (MQ=255)
                                                                                                                          gTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCat                        >  1:1115735/1‑150 (MQ=255)
                                                                                                                            cACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                                  <  2:1278421/74‑1 (MQ=255)
                                                                                                                            cACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                                  <  2:1278424/74‑1 (MQ=255)
                                                                                                                            cACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                                  >  1:1278421/1‑74 (MQ=255)
                                                                                                                            cACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                                  >  1:1278424/1‑74 (MQ=255)
                                                                                                                             aCCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCGAAGGGACTTTGCGCGGCATGCGCAAGCAGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATc                     >  1:926739/1‑150 (MQ=255)
                                                                                                                             aCCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTg                           <  1:403379/144‑1 (MQ=255)
                                                                                                                             aCCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTg                           >  2:403379/1‑144 (MQ=255)
                                                                                                                             aCCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATc                     >  2:475710/1‑150 (MQ=255)
                                                                                                                               ccACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCgg                   >  1:489639/1‑150 (MQ=255)
                                                                                                                                cACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGc                  >  1:1545878/1‑150 (MQ=255)
                                                                                                                                 aCGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCa                 >  2:1482367/1‑150 (MQ=255)
                                                                                                                                   gAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGc               <  2:1644393/150‑1 (MQ=255)
                                                                                                                                       aTGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTgg           >  1:1572653/1‑150 (MQ=255)
                                                                                                                                        agCCGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCg                    <  1:1355134/139‑1 (MQ=255)
                                                                                                                                        agCCGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCg                    >  2:1355134/2‑140 (MQ=255)
                                                                                                                                         gCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGaa         <  2:1615238/150‑1 (MQ=255)
                                                                                                                                         gCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGaa         <  1:475710/150‑1 (MQ=255)
                                                                                                                                          ccGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAg        >  2:1478/1‑150 (MQ=255)
                                                                                                                                           cGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCt                            >  1:327814/1‑129 (MQ=255)
                                                                                                                                           cGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCt                            <  2:327814/129‑1 (MQ=255)
                                                                                                                                            gACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTa      >  1:236779/1‑150 (MQ=255)
                                                                                                                                             aCCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGc                          <  1:500192/129‑1 (MQ=255)
                                                                                                                                             aCCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGc                          >  2:500192/1‑129 (MQ=255)
                                                                                                                                              ccACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAAc    <  2:317172/150‑1 (MQ=255)
                                                                                                                                                aCGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAACCg  >  2:1152584/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
ACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAACCG  >  NC_002947/1416796‑1417089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑