Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 191,924 C→T 7.4% V532I (GTC→ATC)  paxB ← toxin secretion ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947191,9240CT 7.4% 160.4 / 2.9 54V532I (GTC→ATC) paxBtoxin secretion ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (26/24);  new base T (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCCGCG  >  NC_002947/191777‑192059
                                                                                                                                                    |                                                                                                                                       
tGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCcgacga                                                                                                                                       <  1:1620330/150‑1 (MQ=255)
 gACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGAt                                                                                                                                      <  2:29452/150‑1 (MQ=255)
   ccAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGa                                                                                                                                    >  2:455849/1‑150 (MQ=255)
    cAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAg                                                                                                                                   >  2:284204/1‑150 (MQ=255)
        ttGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg                                                                                                                               <  2:166342/150‑1 (MQ=255)
        ttGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg                                                                                                                               >  2:1371318/1‑150 (MQ=255)
          gTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGc                                                                                                                             <  2:1535400/150‑1 (MQ=255)
                    gTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg                                                                                                                               <  2:394160/138‑1 (MQ=255)
                    gTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTg                                                                                                                               >  1:394160/1‑138 (MQ=255)
                        cGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGc                                                                                                               >  1:914984/1‑150 (MQ=255)
                                 gcTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACACCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGc                                                                                                      >  1:538640/1‑150 (MQ=255)
                                    ggATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc                                                                                                   >  2:1149483/1‑150 (MQ=255)
                                       tatCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGc                                                                                                <  2:843954/150‑1 (MQ=255)
                                            tGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCgatg                                                                                           <  2:1333085/150‑1 (MQ=255)
                                                       gCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCtt                                                                                >  2:883565/1‑150 (MQ=255)
                                                          gATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACACCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc                                                                             <  2:538640/150‑1 (MQ=255)
                                                              ttgtGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCa                                                                         <  2:1448970/150‑1 (MQ=255)
                                                                  ggCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGCACTCTTGCCTTAGCCGGTGCGGCCGATGATGCCGACCTTCTCGCCAGGGc                                                                     <  1:284204/150‑1 (MQ=255)
                                                                          tCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTc                                                             <  1:883565/150‑1 (MQ=255)
                                                                               gACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGc                                                                                                               <  2:735201/95‑1 (MQ=255)
                                                                               gACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGc                                                                                                               >  1:735201/1‑95 (MQ=255)
                                                                                 cATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGa                                                      >  2:1545744/1‑150 (MQ=255)
                                                                                                     aCGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc                                                                             <  1:421946/107‑1 (MQ=255)
                                                                                                     aCGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTtctc                                                                             >  2:421946/1‑107 (MQ=255)
                                                                                                          aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc                                                                                                   <  1:640827/80‑1 (MQ=255)
                                                                                                          aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGc                                                                                                   >  2:640827/1‑80 (MQ=255)
                                                                                                          aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGcc                                                                          >  1:1551952/1‑105 (MQ=255)
                                                                                                          aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGcc                                                                          <  2:1551952/105‑1 (MQ=255)
                                                                                                          aTCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgccg                             >  1:66524/1‑148 (MQ=255)
                                                                                                           tCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCga                                                                                             <  2:238540/85‑1 (MQ=255)
                                                                                                           tCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCga                                                                                             >  1:238540/1‑85 (MQ=255)
                                                                                                              aCCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCAGATTc                         <  2:1471274/150‑1 (MQ=255)
                                                                                                                    agcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa                                             >  1:671024/1‑124 (MQ=255)
                                                                                                                    agcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa                                             <  2:671024/124‑1 (MQ=255)
                                                                                                                     gcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGAt                                                                 >  2:1422702/1‑103 (MQ=255)
                                                                                                                     gcGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGAt                                                                 <  1:1422702/103‑1 (MQ=255)
                                                                                                                      cGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCTATTGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGt                 <  1:199972/150‑1 (MQ=255)
                                                                                                                         gCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGaa              >  1:161633/1‑150 (MQ=255)
                                                                                                                          tcatcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgctg                             <  2:501241/128‑1 (MQ=255)
                                                                                                                          tcatcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTctgctg                             >  1:501241/7‑134 (MQ=38)
                                                                                                                           caccaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGt            >  1:198851/1‑150 (MQ=255)
                                                                                                                            accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc           >  1:154024/1‑150 (MQ=255)
                                                                                                                            accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc           <  2:154024/150‑1 (MQ=255)
                                                                                                                            accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc           <  1:1371318/150‑1 (MQ=255)
                                                                                                                            accaTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTc           >  1:1203420/1‑150 (MQ=255)
                                                                                                                             tcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa                                             >  2:347957/4‑115 (MQ=38)
                                                                                                                             tcgtCGGCCT‑GTACGAGGCCGATGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCa                                             <  1:347957/112‑1 (MQ=38)
                                                                                                                               aTCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGAc        <  2:198851/150‑1 (MQ=255)
                                                                                                                                   gCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCcg    >  1:1059969/1‑150 (MQ=255)
                                                                                                                                    ccTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCcgc   >  1:915548/1‑150 (MQ=255)
                                                                                                                                     cTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGATCTGCTGGTTCGGATAAGTGAAGTCGACCAcgcg  >  1:1411474/1‑150 (MQ=255)
                                                                                                                                      tCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATg                                                                <  1:821833/87‑1 (MQ=255)
                                                                                                                                      tCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATg                                                                >  2:821833/1‑87 (MQ=255)
                                                                                                                                             aGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGa                      >  2:533718/1‑123 (MQ=255)
                                                                                                                                             aGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGa                      <  1:533718/123‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                       
TGACCAGGTTGTCACGCAAGGTGCCGGCCAGCAGCTGGATATCCTGGGGGACGTAGCCGATGTTGTGGCGCAGTTCGCTGACATCGATCTGGCGAATGTCGACGCCATCAACCAGCAGCGAGCCACCATCGGCCTCGTAC‑AGGCCGACGATGAGCTTGGCCAGCGAGCTCTTGCCGGAGCCGCTGCGGCCGATGATGCCGACCTTCTCGCCAGGGCGGATGGTCAGGTTGATATTCTTCAGGGCCAGGTTCTGCTGGTTCGGATAAGTGAAGTCGACCCCGCG  >  NC_002947/191777‑192059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑