| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,443,148 | T→C | 7.0% | intergenic (‑79/+97) | ghrB ← / ← PP_1262 | 2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,443,148 | 0 | T | C | 7.0% | 166.0 / 2.7 | 58 | intergenic (‑79/+97) | ghrB/PP_1262 | 2‑ketoaldonate reductase/hydroxypyruvate/glyoxylate reductase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (24/29); new base C (2/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
AAGTCTTGCTGCAGGCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAA > NC_002947/1443002‑1443297 | aaGTCTCGCCGCAGGCGCTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTATCTTTCACTGGGCAACTTTTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTCCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGcc < 2:51856/150‑1 (MQ=255) gTCTTGCTGCAGGCGTTCGGCCATGGCCGGGGTGATACGTCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAAAGCGCTCCTACGGGGGGGCGCGGTGCCtt > 1:1073010/1‑150 (MQ=255) tCTTGCTGCAGGCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGt > 1:1241515/1‑144 (MQ=255) tCTTGCTGCAGGCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGt < 2:1241515/144‑1 (MQ=255) ttGCTGCAGGCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCCCCTACGGGGGGGCGCGGTGCCTTGTa < 2:1502386/150‑1 (MQ=255) tgctgcAGGCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCCTCGGGCAACTTCTCTGCAAGACGGGGTGGGGCTGTGCTCGTCCCTTCGCGGACAAGCCCGCTCCAACGGGGGGGCGCGGGGCATTTTaa > 2:341721/1‑149 (MQ=255) tgcAGGCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGACTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAg > 2:703119/1‑150 (MQ=255) gaaaacccatcaggtgctgatcgccaactaaaaagctgctgcaatgcgggggcggacaatggtaggttgctaaccattgatttcgcctgttccggcaCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCgg > 2:433768‑M1/98‑150 (MQ=255) ggCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCggg < 2:1258587/150‑1 (MQ=255) gCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTa < 2:269312/141‑1 (MQ=255) gCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTa > 1:269312/1‑141 (MQ=255) gTTCGGCCATGGCCGGGGTGATACGGCTGAATGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCgggacg < 1:471335/150‑4 (MQ=255) ggCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAg < 1:895452/150‑1 (MQ=255) ggggTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACAAGGCACCGCGCCCCCCCGTAGGAGCGGGCTTGCCCGCGAAggga > 2:1617196/1‑147 (MQ=21) ggggTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACAAGGCACCGCGCCCCCCCGTAGGAGCGGGCTTGCCCGCGAAggga < 1:1617196/149‑3 (MQ=18) aTACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCCTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAAc > 2:1357775/1‑150 (MQ=255) tACGGCTGAGGGCAAGGAGGTTCTTTTTCACCGGGAACCTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTCCCCGCGAAGGTGCCGGAACa < 2:1552017/150‑1 (MQ=38) aCGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAg < 1:309157/150‑1 (MQ=255) tGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGaa > 2:1257392/1‑150 (MQ=255) aGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACAAGGCACCGCGCCCCCCCGTAGGAGCGGGCTTGCCCGCGAAgggacca > 1:1640772/1‑131 (MQ=14) aGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACAAGGCACCGCGCCCCCCCGTAGGAGCGGGCTTGCCCGCGAAgggacca < 2:1640772/136‑6 (MQ=17) tgctgcaatgcgggggcggacaatggtaggttgctaaccattgatttcgcctgttccggcaCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGa > 1:456335‑M1/62‑110 (MQ=255) tgctgcaatgcgggggcggacaatggtaggttgctaaccattgatttcgcctgttccggcaCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGa < 2:456335‑M1/49‑1 (MQ=255) ggTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGc > 2:1020068/1‑150 (MQ=255) cTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAAc > 2:1442310/1‑150 (MQ=255) cTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGACTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAAc < 1:703119/150‑1 (MQ=255) tCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCgg < 2:838845/95‑1 (MQ=255) tCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCgg > 1:838845/1‑95 (MQ=255) tCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCAtt > 2:825259/1‑150 (MQ=255) aaCTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGc > 1:288810/1‑150 (MQ=255) ttCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGcc > 1:66039/1‑74 (MQ=255) ttCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGcc < 2:66039/74‑1 (MQ=255) ttCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCt > 1:832584/1‑135 (MQ=255) ttCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCt < 2:832584/135‑1 (MQ=255) tctcTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGTGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGccccc < 2:480338/150‑1 (MQ=255) tctcTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGaa < 1:61758/108‑1 (MQ=255) tctcTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGaa > 2:61758/1‑108 (MQ=255) tctcTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGccccc < 1:1442310/150‑1 (MQ=255) ctGCAAGTCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAACCCGGTCCTACGGGGGGGCGCGGTGCCCTTTAGGAGCGGGCTTGCCCGCGAGGGTGCCGGCACCGGCGAAATCCATGTTTAGCAACCTACCCTTGTCCGCCCCCCCa > 2:581727/1‑150 (MQ=255) cattgatttcgcctgttccggcaCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGc > 1:119313‑M1/24‑82 (MQ=255) cattgatttcgcctgttccggcaCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGc < 2:119313‑M1/59‑1 (MQ=255) cGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTgcagca < 1:25329/150‑1 (MQ=255) tGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCgg > 2:1336995/1‑69 (MQ=255) tGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCgg < 1:1336995/69‑1 (MQ=255) ggCTGTGCTGGTTCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTa < 2:1073010/150‑1 (MQ=255) gtgCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTa > 2:346263/1‑112 (MQ=255) gtgCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTa < 1:346263/112‑1 (MQ=255) tGGTCCCTTCGCGGGCAAGCCCGCTCATACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGa > 2:62017/1‑150 (MQ=255) gTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATc > 1:249321/1‑150 (MQ=255) CTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCtttt > 1:1480226‑M1/1‑134 (MQ=255) CTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCtttt < 2:1480226‑M1/134‑1 (MQ=255) TTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCAc < 1:474888‑M1/150‑1 (MQ=255) CCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGtttt < 1:562367‑M1/150‑1 (MQ=255) CCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTc < 1:1423456‑M1/150‑1 (MQ=255) CTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCt < 1:40492‑M1/150‑1 (MQ=255) CGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGcc > 1:62017‑M1/1‑150 (MQ=255) ggggggCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTCCGAACGGGCGCCCCCGCATTGCAGCAGCTCTTTGCATCACGAACAATATCGTATGGGGTTTTAGGTCTGCCTc > 1:1264255/1‑150 (MQ=255) cgcgGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATgcgc < 2:474888/150‑1 (MQ=255) cgcgGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATgcgc < 2:288810/150‑1 (MQ=255) cgGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGACATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCgg < 1:775533/150‑1 (MQ=255) tGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCaa < 2:40492/150‑1 (MQ=255) | AAGTCTTGCTGCAGGCGTTCGGCCATGGCCGGGGTGATACGGCTGAAGGCCAGGACGGTCTTTTTCATCGGGCAACTTCTCTGCAAGGCGGGGTGGGGCTGTGCTGGTCCCTTCGCGGGCAAGCCCGCTCCTACGGGGGGGCGCGGTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGGTGCCGGAACAGGCGAAATCAATGGTTAGCAACCTACCATTGTCCGCCCCCGCATTGCAGCAGCTTTTTAGTTGGCGATCAGCACCTGATGGGTTTTCAGGTCTGCCTCATGCGCGGCAA > NC_002947/1443002‑1443297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |