Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,444,472 T→A 6.2% I30N (ATT→AAT)  PP_1263 → fusaric acid resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,444,4720TA 6.2% 194.9 / 4.3 65I30N (ATT→AAT) PP_1263fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base A (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

CCTAGAATTGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTATGGTGACCCTCAGCTGGACCGCTGG  >  NC_002947/1444337‑1444605
                                                                                                                                       |                                                                                                                                     
ccTAGAATTGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCa                                                                                                                         >  1:300533/1‑150 (MQ=255)
 cTAGAATTGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCaa                                                                                                                        >  2:850688/1‑150 (MQ=255)
  tAGAATTGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCAATGcccc                                                                                                                                 <  2:1395917/140‑1 (MQ=255)
  tAGAATTGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCAATGcccc                                                                                                                                 >  1:1395917/1‑140 (MQ=255)
      aTTGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGc                                                                                                                   <  1:1247316/150‑1 (MQ=255)
       ttGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCt                                                                                                                  <  1:1228432/150‑1 (MQ=255)
         gCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGc                                                                                                                <  1:1240793/150‑1 (MQ=255)
          cccGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCAATGccccaggttc                                                                                                                           >  1:931795/1‑132 (MQ=255)
          cccGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCAATGccccaggttc                                                                                                                           <  2:931795/138‑7 (MQ=255)
                        ctctTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGa                                                                                                 >  2:1270256/1‑150 (MQ=255)
                        ctctTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGa                                                                                                 <  1:1305512/150‑1 (MQ=255)
                              cTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATACGATGGCCGTGCGGTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCg                                                                                           >  2:861932/1‑150 (MQ=255)
                               tCGGGAAATCCCAGCTCTGCCGCGTCGCATCAGCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCCCCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGa                                                                                          >  2:1486077/1‑150 (MQ=255)
                                   gAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGAcgcg                                                                                      >  2:1579885/1‑150 (MQ=255)
                                       tCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGccc                                                                                  >  2:1306327/1‑149 (MQ=255)
                                       tCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCa                                                                                  >  2:1373649/1‑150 (MQ=255)
                                          cAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTc                                                                               <  1:1270256/150‑1 (MQ=255)
                                               gtgCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGaa                                                                          <  2:1541294/150‑1 (MQ=255)
                                                  ccGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGATCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGc                                                                                                            <  2:516307/113‑1 (MQ=255)
                                                  ccGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGATCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGc                                                                                                            >  1:516307/1‑113 (MQ=255)
                                                     cgTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCcgcg                                                                    >  2:689366/1‑150 (MQ=255)
                                                         gCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCaaa                                                                                                                            <  2:774921/90‑1 (MQ=255)
                                                         gCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCaaa                                                                                                                            >  1:774921/1‑90 (MQ=255)
                                                          catcatCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGa                                                               >  1:549800/1‑150 (MQ=255)
                                                           atcatcAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGa                                                               <  2:953093/149‑1 (MQ=255)
                                                           atcatcAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGa                                                               >  1:953093/1‑149 (MQ=255)
                                                              atcaGAACGCTCAAGGCGTTCAGTGATTCTGGCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCt                                                           >  1:1277635/1‑150 (MQ=255)
                                                               tcaGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTc                                                          >  1:1588555/1‑150 (MQ=255)
                                                                    aCGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTgg                                                     <  1:1373649/150‑1 (MQ=255)
                                                                     cGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGc                                                    <  2:1481221/150‑1 (MQ=255)
                                                                      gccttgagCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGcc                                                   >  1:681079/8‑150 (MQ=255)
                                                                      gCTCAAGGCGTTGAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGcc                                                   <  1:1486077/150‑1 (MQ=255)
                                                                        tCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCg                                                 >  2:1581773/1‑150 (MQ=255)
                                                                        gccgggCGTGCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTACCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCg                                                 >  2:408254/5‑150 (MQ=255)
                                                                             gCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACcag                                            <  2:681079/150‑1 (MQ=255)
                                                                               gTTAAGTGCCTGTGTCCTTGCTCTCACTTTGAGCGGCTGTATCGGAACCTGGTGCATTGCTCCGGCAAGCAAGACGCTGCAAGCCAATTCCTTGCACGCCGCCGAGGCTATTCGCGAAGCTGTGTTTGCCGCTCACTGGCACGCCctgca                                          <  2:302632/150‑1 (MQ=255)
                                                                                   aGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTg                                                      >  1:1405701/1‑134 (MQ=255)
                                                                                   aGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTg                                                      <  2:1405701/134‑1 (MQ=255)
                                                                                    gTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGaccac                                                                                             <  1:1528193/94‑1 (MQ=255)
                                                                                    gTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGaccac                                                                                             >  2:1528193/1‑94 (MQ=255)
                                                                                    gTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAgtggt                                     >  2:236401/1‑150 (MQ=255)
                                                                                     tGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGAGCGGCTCTCACTGGCCCGACCAGCAgtggtg                                    <  1:861932/150‑1 (MQ=255)
                                                                                        cTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGAcgcg                                                                                      <  2:313619/97‑1 (MQ=255)
                                                                                        cTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGAcgcg                                                                                      >  1:313619/1‑97 (MQ=255)
                                                                                        cTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGa                                                               <  2:1467030/120‑1 (MQ=255)
                                                                                        cTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGa                                                               >  1:1467030/1‑120 (MQ=255)
                                                                                        cTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGa                                                               >  1:1524379/1‑120 (MQ=255)
                                                                                        cTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGa                                                               <  2:1524379/120‑1 (MQ=255)
                                                                                        cTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCa                                 >  1:1082740/1‑150 (MQ=255)
                                                                                                ttgccctCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTATg                         <  1:245190/150‑1 (MQ=255)
                                                                                                   ccctCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGc                              <  2:1521499/142‑1 (MQ=255)
                                                                                                   ccctCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGc                              >  1:1521499/1‑142 (MQ=255)
                                                                                                    cctCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTATGGTGa                     >  1:435878/1‑150 (MQ=255)
                                                                                                    cctCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCAACCCGCAGTGGTGGCACGCCTATGGTGa                     >  2:1500513/1‑150 (MQ=255)
                                                                                                          cTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGc                                                            <  2:1414930/105‑1 (MQ=255)
                                                                                                          cTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGc                                                            >  1:1414930/1‑105 (MQ=255)
                                                                                                             gAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTATGGTGACCCTCAGCt            <  1:635611/150‑1 (MQ=255)
                                                                                                                    tGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTATGGTGACCCTCAGCTGGACCGc     >  1:750131/1‑150 (MQ=255)
                                                                                                                       aTCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTATGGTGACCCTCAGCTGGACCGCTgg  <  1:689366/150‑1 (MQ=255)
                                                                                                                        tCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTAt                          <  2:245137/125‑1 (MQ=255)
                                                                                                                        tCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTAt                          >  1:245137/1‑125 (MQ=255)
                                                                                                                                tGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAgtg                                       <  1:1291748/104‑1 (MQ=255)
                                                                                                                                tGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAgtg                                       >  2:1291748/1‑104 (MQ=255)
                                                                                                                                    gCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGc                              >  2:425875/1‑109 (MQ=255)
                                                                                                                                    gCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGc                              <  1:425875/109‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CCTAGAATTGCCCGGCCCCTTCACCTCTTCCTCGGGAAATCGCAGCTGTGCCGCGTCGCATCATCAGAACGCTCAAGGCGTTCAGTGCCTGTGCCCTTGCCCTCACCTTGAGCGGCTGTATCGGAACCTGGGGCATTGCCCCGCAAAGCAAGACGCTGCAAGCCAATACCTTGACCACCGACGCGGCCATTCGCGAAGCCGCGACTGACGCTCACTGGCCCGACCAGCAGTGGTGGCACGCCTATGGTGACCCTCAGCTGGACCGCTGG  >  NC_002947/1444337‑1444605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑