| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,458,124 | T→G | 8.5% | intergenic (+17/+23) | PP_1276 → / ← algA | hypothetical protein/mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,458,124 | 0 | T | G | 8.5% | 151.7 / 8.1 | 59 | intergenic (+17/+23) | PP_1276/algA | hypothetical protein/mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (29/25); new base G (2/3); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.48e-01 | |||||||||||
TCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCGATGAT > NC_002947/1457980‑1458269 | tCGAAATGACTTCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGcc > 1:1583634/1‑150 (MQ=255) gAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCa > 2:1344537/1‑150 (MQ=255) cTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGa < 1:1267142/150‑1 (MQ=255) ccACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTc > 1:962628/1‑150 (MQ=255) acaAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGCCAg < 2:1258797/150‑1 (MQ=255) caAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCTGACTCGCCCCTGGGCTGACCAGGGGCGAgcctgc < 1:948224/150‑6 (MQ=38) caAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCACGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGc < 1:1428972/150‑1 (MQ=255) aaGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCa > 2:773410/1‑150 (MQ=255) tCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTAc > 2:1445449/1‑150 (MQ=255) tCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTAc > 1:1375082/1‑150 (MQ=255) aaTCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCACATTCGACATCTACGTCATAGCCCCGCGTGTTGAATATGGAGGCTGGCCCCTGGTCAGCCCATGGGCGACTAAGCATCTACAGCTCGATg > 2:385574/1‑150 (MQ=255) ttCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGt < 1:1175025/147‑1 (MQ=255) ttCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGt > 2:1175025/1‑147 (MQ=255) tttGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGATCGCCCCTGGTCAGCCCAGGGGCGAGTCAGTATTTACTGCGCGATGGTCTTCACCGa < 1:1066877/150‑1 (MQ=255) aaGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAggg < 1:217966/110‑1 (MQ=255) aaGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAggg > 2:217966/1‑110 (MQ=255) gggAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAAcgccg < 2:454147/150‑1 (MQ=255) ggaggaTGATTTGCAGCGAACTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAAcgccgc < 2:1106431/150‑1 (MQ=255) tGCAGCGACCTTTCAGGGTGGTGGGTCGCCCATTCAACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCCGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGGCTTCACCGAAACGCCGCGCTCGATgggg < 1:385574/150‑1 (MQ=255) gCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTgg > 2:53296/1‑150 (MQ=255) cGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGa < 1:892269/150‑1 (MQ=255) cTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATg > 2:869268/1‑111 (MQ=255) cTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATg < 1:869268/111‑1 (MQ=255) cTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTg > 1:1017215/1‑150 (MQ=255) tttCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGa > 2:1558756/1‑89 (MQ=255) tttCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGa < 1:1558756/89‑1 (MQ=255) tttCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTgc > 2:750281/1‑150 (MQ=255) tttCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTgc > 2:821148/1‑150 (MQ=255) tttCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTgc > 1:1174715/1‑150 (MQ=255) ggttggtggGTGGCCCATTCGACATCTACGTCCCAGCCAACAGCTTTTAATAAGCATGCTCGCCCCTGGTCAATATAAGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCa < 1:773410/147‑1 (MQ=255) gggtggCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACAt > 2:872116/1‑150 (MQ=255) ggtggCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGAt > 1:730712/1‑123 (MQ=255) ggtggCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGAt < 2:730712/123‑1 (MQ=255) cccATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGTGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACAACCTCGa < 1:1344537/150‑1 (MQ=255) ccATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa > 1:340124/1‑150 (MQ=255) ttCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGAc > 1:714683/1‑124 (MQ=255) ttCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGAc < 2:714683/124‑1 (MQ=255) aTCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGc > 2:851150/1‑51 (MQ=255) aTCTACCGCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGc < 1:851150/51‑1 (MQ=255) tACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTc > 2:72123/1‑150 (MQ=255) aGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGc < 1:408142/150‑1 (MQ=255) gCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa < 2:1094436/129‑1 (MQ=255) gCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa > 1:1094436/1‑129 (MQ=255) ccccGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGccc < 1:1445449/150‑1 (MQ=255) gAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGt < 1:53296/150‑1 (MQ=255) gAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTccc > 1:1499292/1‑150 (MQ=255) gCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGTGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTg > 2:375125/1‑150 (MQ=255) cAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGc < 2:962628/150‑1 (MQ=255) gacTCGCCCCTGGGCTGACCAGGGGCGAGCCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGccc > 2:230473/3‑93 (MQ=37) gacTCGCCCCTGGGCTGACCAGGGGCGAGCCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGccc < 1:230473/91‑1 (MQ=37) tCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTc > 2:1507476/1‑150 (MQ=255) tCGCCCCTGGGCTGACCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCg < 2:774309/112‑1 (MQ=255) tCGCCCCTGGGCTGACCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCAATACACATCCTCGAAGCGCTCg > 1:774309/1‑112 (MQ=255) gCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAACCGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCGCGATAGCATCCTCGCCCAGGTATCTCCCGGACTGCACCTCga > 2:1320151/1‑150 (MQ=255) ccccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCgat < 1:72123/150‑1 (MQ=255) cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGcc < 2:46983/122‑1 (MQ=255) cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGcc > 1:46983/1‑122 (MQ=255) cccTGGTCAGCCCAGGGGCGAGTCAGCATGTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCgatg < 1:821148/150‑1 (MQ=255) cTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCgatgat > 2:611892/1‑150 (MQ=255) | TCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCGATGAT > NC_002947/1457980‑1458269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |