| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,691,825 | C→A | 26.9% | intergenic (+50/‑121) | PP_1487 → / → PP_1488 | hypothetical protein/methyl‑accepting chemotaxis transducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,691,825 | 0 | C | A | 26.9% | 121.2 / 37.0 | 63 | intergenic (+50/‑121) | PP_1487/PP_1488 | hypothetical protein/methyl‑accepting chemotaxis transducer |
| Reads supporting (aligned to +/- strand): ref base C (27/19); new base A (8/9); total (35/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCTTGC > NC_002947/1691681‑1691964 | aGCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTgc < 2:339697/150‑1 (MQ=255) gCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGAAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTgcg < 2:983041/150‑1 (MQ=255) ccGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGCGCGCCTGTGAGATCGAGCGCCGCCCGTgcg < 1:1204588/149‑1 (MQ=255) gCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGgcgc > 2:47985/1‑150 (MQ=255) gCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGgcgc > 2:347667/1‑150 (MQ=255) ccTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTcgat < 2:224584/150‑4 (MQ=39) cccGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTCGATCTCACAGgcgctca < 1:759657/150‑4 (MQ=37) ccGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCaaaa > 1:1373563/1‑150 (MQ=255) gCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTCGATCTcac > 1:1516972/1‑134 (MQ=37) gCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTCGATCTcac < 2:1516972/134‑1 (MQ=37) tGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGGGCGCCGCACGCGCGGCGCTCGATCTcaca > 2:718239/1‑133 (MQ=38) tGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGGGCGCCGCACGCGCGGCGCTCGATCTcaca < 1:718239/133‑1 (MQ=38) gCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACa > 1:133983/1‑150 (MQ=255) aCCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATcac < 1:292365/150‑1 (MQ=255) ccGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACAt < 2:1599038/150‑1 (MQ=255) ccGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACAt > 2:1423725/1‑150 (MQ=255) ccGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGAGAAATTCCTGACGACAATCACAt > 1:1547389/1‑150 (MQ=255) cGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCCGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATc < 1:1406498/150‑1 (MQ=255) ttGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATAc < 1:232523/150‑1 (MQ=255) aCTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTcgat < 1:1157977/99‑4 (MQ=25) aCTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTcgat > 2:1157977/1‑96 (MQ=25) tGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTg > 2:1115800/1‑150 (MQ=255) tGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTg > 2:51934/1‑150 (MQ=255) gTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGTGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTCGATCTCACAGgc > 1:44825/1‑95 (MQ=17) gTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGTGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTCGATCTCACAGgc < 2:44825/95‑1 (MQ=17) gTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTc > 2:822751/1‑150 (MQ=255) tGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGACCGCAACCCGCGGGATGTTTCGTCTCACAGGGGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCg < 1:198399/150‑1 (MQ=255) ggTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACctct > 2:254145/1‑134 (MQ=255) ggTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACctct < 1:254145/134‑1 (MQ=255) gTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGtc < 1:1156478/82‑1 (MQ=255) gTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGtc > 2:1156478/1‑82 (MQ=255) gTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGa < 2:1506830/150‑1 (MQ=255) ttGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAt < 1:51934/150‑1 (MQ=255) ttGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAt > 2:1477887/1‑150 (MQ=255) ggCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAttt > 2:1302001/1‑150 (MQ=255) gCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGc > 1:453898/1‑150 (MQ=255) tAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCg < 2:1373563/150‑1 (MQ=255) gTGGCGGTGCTTGCCACGGCCTATTGGGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGtt > 1:1516684/1‑150 (MQ=255) ggCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTtgt < 1:347667/150‑1 (MQ=255) ttGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCt > 1:636857/1‑150 (MQ=255) ttGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCt < 2:453898/150‑1 (MQ=255) gCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGa > 1:221897/1‑150 (MQ=255) cACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTc > 1:464416/1‑70 (MQ=255) cACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTc < 2:464416/70‑1 (MQ=255) ccTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGa > 1:1521722/1‑150 (MQ=255) aTTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACATCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAAtg > 1:1088446/1‑150 (MQ=255) gcCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGa > 2:1091449/1‑106 (MQ=255) gcCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGa < 1:1091449/106‑1 (MQ=255) gcCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTgaagaa > 1:81879/1‑150 (MQ=255) ccTGTGAGACGGAGCGCCGCACGGGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGc < 2:1564521/103‑1 (MQ=37) ccTGTGAGACGGAGCGCCGCACGGGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGc > 1:1564521/1‑103 (MQ=37) cTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACt > 1:1308092/1‑150 (MQ=255) tgtgAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAtt > 2:668341/1‑105 (MQ=255) tgtgAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAtt < 1:668341/105‑1 (MQ=255) tgtgAGACGGAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTAAATGTGAAGAACTg > 2:261766/1‑150 (MQ=38) tgAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGa > 2:1099376/1‑150 (MQ=255) gagaTCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGAc > 1:1329727/1‑150 (MQ=255) agaTCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGAcc > 2:61520/1‑150 (MQ=255) acggAGCGCCGCACGGGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATcaca < 2:1387830/58‑1 (MQ=21) acggAGCGCCGCACGGGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATcaca > 1:1387830/4‑61 (MQ=21) ggAGCGCCGCACGGGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTgg > 1:1278654/2‑80 (MQ=38) ggAGCGCCGCACGGGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTgg < 2:1278654/79‑1 (MQ=38) cGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCTTgc < 1:47985/150‑1 (MQ=255) | AGCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCTTGC > NC_002947/1691681‑1691964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |