Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,755,417	T→C	5.7%	intergenic (+90/‑110)	PP_1558 → / → PP_1559	hypothetical protein/holin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,755,417	0	T	C	5.7%	211.8 / 2.3	70	intergenic (+90/‑110)	PP_1558/PP_1559	hypothetical protein/holin
Reads supporting (aligned to +/- strand):  ref base T (36/30);  new base C (2/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

GGAAGGCCGGCGTGGCCTGGATCGACGGCGCGATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCTGTGGGCCG  >  NC_002947/1755269‑1755566                                                                                                                                                     |                                                                                                                                                       ggAAGGCCGGCGTGGCCTGGATCGACGGCGCGATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTc                                                                                                                                                       <  2:1197252/150‑1 (MQ=255)          gCGTGGCCTGGATCGACGGCGCGATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGc                                                                                                                                             >  2:963578/1‑150 (MQ=255)                cccacaggccaggatccttctcgggcatgtttggcatctcgattcctcccttttggggagcggagtaggttcggccccaacagcactcccagctcggggcgatgggtgtggtggagccgaaaacgaaaaAGCCTCGGC‑ATTCGCCGGGGc                                                                                                                                       >  1:172851‑M1/130‑150 (MQ=255)                ccTGGATCGACGGCGCGATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGc                                                                                                                                       >  1:1039457/1‑150 (MQ=255)                      tCGACGGCGCGATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTttttc                                                                                                                                 <  1:1485212/150‑1 (MQ=255)                            gcgcGATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTttttcgttttc                                                                                                                           >  2:1275038/1‑150 (MQ=255)                             cgcgATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTttttcgttttcg                                                                                                                          <  1:58524/150‑1 (MQ=255)                                gATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCggg                                                                                                                                         >  2:243076/1‑132 (MQ=255)                                gATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCggg                                                                                                                                         <  1:243076/132‑1 (MQ=255)                                  ttGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTcc                                                                                                                     <  1:960638/150‑1 (MQ=255)                                                   gcagCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGcccc                                                                                                    >  1:882201/4‑150 (MQ=255)                                                      gCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAg                                                                                                 >  1:138702/1‑150 (MQ=255)                                                                        tCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAGACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgggg                                                                               >  1:1223497/1‑150 (MQ=255)                                                                        tCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTggtg                                                                               >  2:289679/1‑148 (MQ=255)                                                                        tCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgggg                                                                               <  1:985799/150‑1 (MQ=255)                                                                        tCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgggg                                                                               >  1:1398422/1‑150 (MQ=255)                                                                                aTCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgg                                                                                 <  2:1163249/140‑1 (MQ=255)                                                                                aTCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgg                                                                                 >  1:1163249/1‑140 (MQ=255)                                                                                    ttCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTc                                                                   >  2:133083/1‑150 (MQ=255)                                                                                        tgtTACTTTGCGGTAGGTGCGCGAGAGAAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGCGCCGAACCTACTCCGCt                                                               >  2:328292/1‑150 (MQ=255)                                                                                         gtTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTttttcgtt                                                                                                                              <  1:58497/86‑1 (MQ=255)                                                                                         gtTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTttttcgtt                                                                                                                              >  2:58497/1‑86 (MQ=255)                                                                                           tACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCAACAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTccc                                                            >  1:967313/1‑150 (MQ=255)                                                                                              tttGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGt                                                                                    <  1:1545463/123‑1 (MQ=255)                                                                                              tttGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGt                                                                                    >  2:1545463/1‑123 (MQ=255)                                                                                              tttGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgg                                                                                 >  2:747372/1‑126 (MQ=255)                                                                                              tttGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgg                                                                                 <  1:747372/126‑1 (MQ=255)                                                                                              tttGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCaa                                                         >  1:13907/1‑150 (MQ=255)                                                                                              tttGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCaa                                                         >  2:530769/1‑150 (MQ=255)                                                                                                  cGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACACAGCCCCCCAAAAgg                                                     <  2:882201/150‑1 (MQ=255)                                                                                                   ggTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGa                                                                                           >  1:890569/1‑111 (MQ=255)                                                                                                   ggTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGa                                                                                           <  2:890569/111‑1 (MQ=255)                                                                                                               agagCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGAAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGAt                                        <  1:1275038/150‑1 (MQ=255)                                                                                                                  gCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTc                                                                   <  2:362008/120‑1 (MQ=255)                                                                                                                  gCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTc                                                                   >  1:362008/1‑120 (MQ=255)                                                                                                                      aCTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGGACCTACTCCGCTCCCCAAAAGGGAGGAATTGAGATGCCAAAc                                 >  1:598231/1‑150 (MQ=255)                                                                                                                        tCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTgg                                                                                             <  1:1201367/88‑1 (MQ=255)                                                                                                                        tCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTgg                                                                                             >  2:1201367/1‑88 (MQ=255)                                                                                                                        tCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAgg                                                     >  1:1137441/1‑128 (MQ=255)                                                                                                                        tCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAgg                                                     <  2:1137441/128‑1 (MQ=255)                                                                                                                        tCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGgga                                                   >  1:735444/1‑130 (MQ=255)                                                                                                                        tCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGgga                                                   <  2:735444/130‑1 (MQ=255)                                                                                                                          gCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTgg                                                                                             <  1:190875/86‑1 (MQ=255)                                                                                                                          gCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTgg                                                                                             >  2:190875/1‑86 (MQ=255)                                                                                                                          gCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGc                             >  1:682765/1‑150 (MQ=255)                                                                                                                          gCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGc                             >  2:494818/1‑150 (MQ=255)                                                                                                                           ccATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGcc                            >  1:319378/1‑150 (MQ=255)                                                                                                                            cATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGccc                           <  1:676124/150‑1 (MQ=255)                                                                                                                             aTCACCAGCAGACTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCg                          >  1:412671/1‑150 (MQ=255)                                                                                                                              gtggagccgaaaacgaaaaAGCCCCGGCGAAT‑GCCGAGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgg                                                                                 >  1:1607784‑M1/20‑94 (MQ=255)                                                                                                                              gtggagccgaaaacgaaaaAGCCCCGGCGAAT‑GCCGAGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTgg                                                                                 <  2:1607784‑M1/75‑1 (MQ=255)                                                                                                                                aCCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCCCGCCTACTCCGCTCCCCAACAGGGAGGAATCGAGAGGCCAAACATGGCCgaga                       <  2:1223497/150‑1 (MQ=255)                                                                                                                                 ccAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGaa                      <  1:289679/150‑1 (MQ=255)                                                                                                                                 ccAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGaa                      <  2:832430/150‑1 (MQ=255)                                                                                                                                 ccAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGaa                      <  1:1468334/150‑1 (MQ=255)                                                                                                                                  cagcagCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTg                                                                                  >  1:147910/1‑89 (MQ=255)                                                                                                                                  cagcagCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTg                                                                                  <  2:147910/89‑1 (MQ=255)                                                                                                                                   gccgaaaacgaaaaAGCCCCGGCGAAT‑GCCGAGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCgaga                                         >  2:1327911‑M1/15‑129 (MQ=255)                                                                                                                                   gccgaaaacgaaaaAGCCCCGGC‑GAATGCCGAGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCgaga                                         <  1:1327911‑M1/115‑1 (MQ=255)                                                                                                                                       gcCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATcc                <  2:1039457/150‑1 (MQ=255)                                                                                                                                         ctcctTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTg              >  2:666604/1‑150 (MQ=255)                                                                                                                                          tcctTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTgg             >  1:1340328/1‑150 (MQ=255)                                                                                                                                             ttAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGa                                                                                           >  1:1355958/1‑69 (MQ=255)                                                                                                                                             ttAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGa                                                                                           <  2:1355958/69‑1 (MQ=255)                                                                                                                                                gagCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCtgtg       <  2:138702/150‑1 (MQ=255)                                                                                                                                                 AGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCTGTgg      >  1:963578‑M1/1‑150 (MQ=255)                                                                                                                                                   CCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGatcctggcccacaggc    <  1:110655‑M1/150‑8 (MQ=255)                                                                                                                                                   CCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCTGTGGGc    >  2:1484522‑M1/1‑150 (MQ=255)                                                                                                                                                   CCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCTGTGGGc    <  1:698960‑M1/150‑1 (MQ=255)                                                                                                                                                   CCTCGGC‑ATTCGACGGGGCCTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCTGTGGGc    <  2:401982‑M1/150‑1 (MQ=255)                                                                                                                                                     tCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCAACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCTGTGGGCCg  >  1:1134959/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                       GGAAGGCCGGCGTGGCCTGGATCGACGGCGCGATTGATATTCGTCGTGAAGCCGCTTGACAGCCCCGGTCCTCACCCGTATCTTTCGTGTTACTTTGCGGTAGGTGCGCGAGAGCAAACTCGCCATCACCAGCAGCCTCCTTAGAGCCTCGGC‑ATTCGCCGGGGCTTTTTCGTTTTCGGCTCCACCACACCCATCGCCCCGAGCTGGGAGTGCTGTTGGGGCCGAACCTACTCCGCTCCCCAAAAGGGAGGAATCGAGATGCCAAACATGCCCGAGAAGGATCCTGGCCTGTGGGCCG  >  NC_002947/1755269‑1755566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑