Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,842,594	C→T	5.9%	V748I (GTC→ATC)	gacS ←	sensor protein GacS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,842,594	0	C	T	5.9%	202.1 / 2.5	68	V748I (GTC→ATC)	gacS	sensor protein GacS
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base T (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CTGCTGCGGTGTGCCCAGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCT  >  NC_002947/1842445‑1842722                                                                                                                                                      |                                                                                                                                 ctgctgCGGTGTCCCCAGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCAAAATGCCGCGCATTGGACGGGGTAGTTAGGTAGTCGTCCATGACGCTGTGCAACAACGTGCGCTTTTCGTTGGCCATGGCATGGGCAGTGAGGGcgac                                                                                                                                  <  1:1606576/150‑1 (MQ=255)     tgcGGTGTGCCCAGGCTCAGGCCGGTCCACTTCATCACCACCTGTGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATc                                                                                                                              <  1:536642/150‑1 (MQ=255)     tgcGGTGTGCCCAGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATc                                                                                                                              <  1:162233/150‑1 (MQ=255)               ccAGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTGTCGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGt                                                                                                                    >  2:1042099/1‑150 (MQ=255)                cAGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGtt                                                                                                                   <  1:1166089/150‑1 (MQ=255)                cAGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGtt                                                                                                                   >  1:526394/1‑150 (MQ=255)                 aGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTg                                                                                                                  >  1:1080896/1‑150 (MQ=255)                         gCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTg                                                                                                          >  1:718395/1‑150 (MQ=255)                          ccGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGTTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         <  1:455680/150‑1 (MQ=255)                          ccGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         <  1:959730/150‑1 (MQ=255)                          ccGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         <  1:1512785/150‑1 (MQ=255)                          ccGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         <  2:927612/150‑1 (MQ=255)                           cGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTCCCGCTCTggg                                                                                                        <  1:1042099/150‑1 (MQ=255)                            ggTCCACTTCATCACCACCTGGGTCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGgt                                                                                                       <  2:598918/150‑1 (MQ=255)                               ccACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGtt                                                                                                    <  2:718395/150‑1 (MQ=255)                                cACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGATGATCGGCAGCGGGTTGCCGCTCTGGGTGTtc                                                                                                   >  1:393592/1‑150 (MQ=255)                                 aCTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         >  2:1219583/1‑143 (MQ=255)                                 aCTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         <  1:1219583/143‑1 (MQ=255)                                                   ccAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGAt                                                                                     >  2:657181/1‑145 (MQ=255)                                                   ccAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGAt                                                                                     <  1:657181/145‑1 (MQ=255)                                                            gTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCgg                                                                             <  2:1266424/144‑1 (MQ=255)                                                            gTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCgg                                                                             >  1:1266424/1‑144 (MQ=255)                                                               cGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGgcgc                                                                    <  2:526394/150‑1 (MQ=255)                                                                    aTCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         >  1:1108627/1‑108 (MQ=255)                                                                    aTCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTgg                                                                                                         <  2:1108627/108‑1 (MQ=255)                                                                    aTCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACa                                                                         >  2:1351516/1‑140 (MQ=255)                                                                    aTCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACa                                                                         <  1:1351516/140‑1 (MQ=255)                                                                             gTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCg                                                      >  2:1091718/1‑150 (MQ=255)                                                                             gTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCGGTTCGGTACAGGCGCGGCCGTCCATGCCg                                                      >  2:1026187/1‑150 (MQ=255)                                                                              tCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGAGCTGTTCGGTACAGGCGCGGCCGTCCATGCCgg                                                     >  2:1620683/1‑150 (MQ=255)                                                                                aGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGtt                                                                                                    >  2:50793/1‑101 (MQ=255)                                                                                aGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGtt                                                                                                    <  1:50793/101‑1 (MQ=255)                                                                                  gTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTTTTTTTTTTTTTCATGAGCAGTGAGGGTGACGATTTGCAGCGGGTTGCCGCTCTTGGTGTTTTCCCTCTTTCTGATCTGTTCGGTACCGGCGCGGCCGTCCATGCCgtccat                                                 >  2:703884/1‑145 (MQ=255)                                                                                  gTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCAt                                                 >  2:1432776/1‑150 (MQ=255)                                                                                      tcgtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGAt                                                                                                                               <  1:1593759/68‑1 (MQ=255)                                                                                      tcgtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGAt                                                                                                                               >  2:1593759/1‑68 (MQ=255)                                                                                      tcgtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTtc                                                                                                   >  1:328106/1‑96 (MQ=255)                                                                                      tcgtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTtc                                                                                                   <  2:328106/96‑1 (MQ=255)                                                                                        gtcCATGTCGCTGTGCAACACCGGGCGTTTTTCGTTGGCCATGGCATGGGCAGGGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGcac                                           <  2:510208/150‑1 (MQ=255)                                                                                        gtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGctc                                                                                                            >  1:1182025/1‑85 (MQ=255)                                                                                        gtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGctc                                                                                                            <  2:1182025/85‑1 (MQ=255)                                                                                        gtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGt                                                                            >  1:890064/1‑117 (MQ=255)                                                                                        gtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGt                                                                            <  2:890064/117‑1 (MQ=255)                                                                                        gtcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGcac                                           >  1:268417/1‑150 (MQ=255)                                                                                         tcCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGcaca                                          >  2:739379/1‑150 (MQ=255)                                                                                           cATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcatc                                        >  2:144980/1‑150 (MQ=255)                                                                                           cATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcatc                                        >  1:1626094/1‑150 (MQ=255)                                                                                               ccGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGTTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcatccatc                                    <  1:1432776/150‑1 (MQ=255)                                                                                                cGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCa                                   >  2:256688/1‑150 (MQ=255)                                                                                                 gCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAg                                  >  2:788830/1‑150 (MQ=255)                                                                                                   tgtgCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGcc                                                                                                                >  2:840559/1‑70 (MQ=255)                                                                                                   tgtgCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGcc                                                                                                                <  1:840559/70‑1 (MQ=255)                                                                                                         acaacGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGATGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTg                                              >  2:1447898/1‑130 (MQ=255)                                                                                                         acaacGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGATGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTg                                              <  1:1447898/130‑1 (MQ=255)                                                                                                                gcgTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACaa                   >  1:134639/1‑150 (MQ=255)                                                                                                                  gTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGAt                                                                                     >  2:745107/1‑82 (MQ=255)                                                                                                                  gTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGAt                                                                                     <  1:745107/82‑1 (MQ=255)                                                                                                                   tttttCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAAttc                <  2:1313035/150‑1 (MQ=255)                                                                                                                      ttCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCTCCAGGTCGAACAAttcttc             >  2:1081780/1‑150 (MQ=255)                                                                                                                      ttCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAAttcttc             <  1:256688/150‑1 (MQ=255)                                                                                                                        cGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCt           <  2:1570776/150‑1 (MQ=255)                                                                                                                          ttGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcatc                                        <  2:112939/119‑1 (MQ=255)                                                                                                                          ttGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCAcatc                                        >  1:112939/1‑119 (MQ=255)                                                                                                                            ggCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCAc       <  1:739379/150‑1 (MQ=255)                                                                                                                              ccATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACgg     <  2:572497/150‑1 (MQ=255)                                                                                                                              ccATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTACTTCACgg     <  1:144980/150‑1 (MQ=255)                                                                                                                               catggcatggGCAGTGAGGGCGATGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGc    <  2:393592/150‑1 (MQ=255)                                                                                                                               catggcatggGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACCGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGc    <  2:955784/150‑1 (MQ=255)                                                                                                                                 tggcatggGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCNATCAGCACNAGGTCGAACAATTCTTCCTGCACGGccn  <  1:788830/150‑2 (MQ=255)                                                                                                                                                      |                                                                                                                                 CTGCTGCGGTGTGCCCAGGCTCAGGCCGGTCCACTTCATCACCACCTGGGCCAGTTGCCGTTCGCTGATCGGTTTGGTCAGGTAGTCGTCCATGCCGCTGTGCAACAACGCGCGTTTTTCGTTGGCCATGGCATGGGCAGTGAGGGCGACGATCGGCAGCGGGTTGCCGCTCTGGGTGTTCTCCCACAGGCGGATCTGTTCGGTACAGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCT  >  NC_002947/1842445‑1842722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑