| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,851,994 | G→T | 100% | E346D (GAG→GAT) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,851,994 | 0 | G | T | 100.0% | 152.9 / ‑6.8 | 47 | E346D (GAG→GAT) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (26/20); minor base A (1/0); total (27/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGG > NC_002947/1851853‑1852135 | cTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGAt < 2:1480957/150‑1 (MQ=255) cacCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTAc > 2:604318/1‑150 (MQ=255) gCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGc < 2:768393/150‑1 (MQ=255) aTCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCAc > 2:1405191/1‑150 (MQ=255) tCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACg > 1:347634/1‑150 (MQ=255) acCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGcc > 1:1528878/1‑150 (MQ=255) ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGaa < 1:173495/125‑1 (MQ=255) ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGaa > 2:173495/1‑125 (MQ=255) gAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGCACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTgg > 1:671450/1‑150 (MQ=255) aGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGCACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc < 2:671450/150‑1 (MQ=255) cgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACaa > 2:1195479/1‑150 (MQ=255) acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg < 2:1528878/150‑1 (MQ=255) acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg < 2:300176/150‑1 (MQ=255) acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg < 1:349501/150‑1 (MQ=255) cTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGCGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAggg > 2:1364024/1‑150 (MQ=255) cAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcgc > 2:602096/1‑123 (MQ=255) cAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcgc < 1:602096/123‑1 (MQ=255) ccccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCaa < 1:1195479/150‑1 (MQ=255) cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc < 1:158289/127‑1 (MQ=255) cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc > 2:158289/1‑127 (MQ=255) ccAAGGAGAACGGCTACCGCTGGTGGCAGACTGCGGGAATCGGCCCCGAGGGCAAGGTGCTGGGTGTGCAGAGCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGc > 2:919999/1‑150 (MQ=255) aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc < 1:1430687/107‑1 (MQ=255) aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc > 2:1430687/1‑107 (MQ=255) aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCa > 2:918754/1‑150 (MQ=255) aaCGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTcc > 1:1449916/1‑150 (MQ=255) cGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAagag > 2:1623644/1‑88 (MQ=255) cGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAagag < 1:1623644/88‑1 (MQ=255) tACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGAGACCACTGAAGCTGTGAGGGTATCGACGGCAAGCCCAGCTCCAACCAc > 2:14701/1‑150 (MQ=255) cGCTCGATGCACACTGCGGTAATCGGCCCCGAGGGCAAGGAGATGGAATGGCGGGTCCTGACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAAGGCACCGACTGCAAGCCGAGCTCCAACCACTAc > 2:639273/1‑150 (MQ=255) gCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACg > 1:1272451/1‑150 (MQ=255) acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAtt > 1:72280/1‑150 (MQ=255) tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg > 1:179460/1‑104 (MQ=255) tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg < 2:179460/104‑1 (MQ=255) tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc > 1:1153788/1‑150 (MQ=255) cGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa > 2:468727/1‑142 (MQ=255) cGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa < 1:468727/142‑1 (MQ=255) ggTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg < 2:1272451/150‑1 (MQ=255) tCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcgc > 2:1569478/1‑89 (MQ=255) tCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcgc < 1:1569478/89‑1 (MQ=255) tCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGc > 2:1386939/1‑150 (MQ=255) gCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc < 2:1449916/150‑1 (MQ=255) cccGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGCACTTGACATATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAg < 1:604318/150‑1 (MQ=255) gAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCTAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTg < 1:378333/150‑1 (MQ=255) ggTGCTGTATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTg > 2:1100509/1‑150 (MQ=255) gTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTgg > 2:1554729/1‑150 (MQ=255) cTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg > 2:656063/1‑125 (MQ=255) cTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg < 1:656063/125‑1 (MQ=255) | CTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGG > NC_002947/1851853‑1852135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |