Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,910,832 G→A 5.5% R8C (CGC→TGC)  PP_1710 ← phthalate permease family MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,910,8320GA 5.5% 223.3 / 2.4 72R8C (CGC→TGC) PP_1710phthalate permease family MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (28/40);  new base A (2/2);  total (30/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCCATGAACGGCGCTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTGGAGGCGGGGCA  >  NC_002947/1910684‑1910974
                                                                                                                                                    |                                                                                                                                              
gCCATGAACGGCGCTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGAcgcggcgcgg                                                                                                                                               >  2:97478/1‑150 (MQ=255)
   aTGAACGGCGCTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGGGTCCAGGGTGTACAACGCAGCAACGCggggg                                                                                                                                            >  2:758060/1‑150 (MQ=255)
   aTGAACGGCGCTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCggggg                                                                                                                                            >  1:1407373/1‑150 (MQ=255)
     gAACGGCGCTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGt                                                                                                                                          <  1:262597/150‑1 (MQ=255)
       aCGGCGCGGCGATAGAAGTGTTCACCCGGACGTCTTAGTTGATTATGGTCGCCACCAGCAGCGGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGTCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAg                                                                                                                                        <  2:698832/150‑1 (MQ=255)
            gcTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTg                                                                                                                                   >  2:472410/1‑150 (MQ=255)
            gcTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTg                                                                                                                                   <  1:1463402/150‑1 (MQ=255)
                 gATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGa                                                                                                                              >  2:1416001/1‑150 (MQ=255)
                   tGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACa                                                                                                                            <  2:1310094/150‑1 (MQ=255)
                    ggAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACAt                                                                                                                           <  1:368560/150‑1 (MQ=255)
                      aaaTGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACCGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTATACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGa                                                                                                                         <  1:1568382/150‑1 (MQ=255)
                         tGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGAAAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTc                                                                                                                      <  1:1427889/150‑1 (MQ=255)
                           ttCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGa                                                                                                                    >  2:1560016/1‑150 (MQ=255)
                           ttCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGa                                                                                                                    <  1:555455/150‑1 (MQ=255)
                               cccGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTc                                                                                                                <  1:127087/150‑1 (MQ=255)
                                ccGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCg                                                                                                               >  1:869105/1‑150 (MQ=255)
                                      gACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTc                                                                                                                      >  2:343976/1‑137 (MQ=255)
                                      gACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTc                                                                                                                      <  1:343976/137‑1 (MQ=255)
                                      gACGTAGTTGATGATGATCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTttgtt                                                                                                         >  1:1136456/1‑150 (MQ=255)
                                       aCGTAGTTGATGATGATCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTttgttg                                                                                                        <  2:1136456/150‑1 (MQ=255)
                                          tAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTttgttgttg                                                                                                     <  2:869105/150‑1 (MQ=255)
                                            gTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTCCGACGACTCGTCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTgta                                                                                                   <  2:400852/150‑1 (MQ=255)
                                               gatgatGGTCGCCACCAGCAGCAGTGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGttt                                                                                                            >  1:325650/1‑138 (MQ=255)
                                               gatgatGGTCGCCACCAGCAGCAGTGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGttt                                                                                                            <  2:325650/138‑1 (MQ=255)
                                               gatgatGGTCGCCACCAGCAGCAGTGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGttt                                                                                                            <  2:1145410/138‑1 (MQ=255)
                                               gatgatGGTCGCCACCAGCAGCAGTGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGttt                                                                                                            >  1:1145410/1‑138 (MQ=255)
                                               gatgatGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTgtaggt                                                                                                >  2:1273538/1‑150 (MQ=255)
                                                 tgatgGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTgtaggtag                                                                                              >  2:1472878/1‑150 (MQ=255)
                                                  gatgGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCgggg                                                                                                                                             >  2:1184650/1‑102 (MQ=255)
                                                  gagtgTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCgggg                                                                                                                                             <  1:1184650/98‑1 (MQ=255)
                                                      gTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATTAGTCGACCTCGTTTGTTGTTgtagg                                                                                                 >  2:135594/1‑142 (MQ=255)
                                                      gTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATTAGTCGACCTCGTTTGTTGTTgtagg                                                                                                 <  1:135594/142‑1 (MQ=255)
                                                         gCCACCAGCAGCATCGACAGCAGGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTggg                                                                                      <  2:912063/150‑1 (MQ=255)
                                                         gCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTggg                                                                                      >  1:855810/1‑150 (MQ=255)
                                                          ccaccaGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGa                                                                                                                         <  1:743934/114‑1 (MQ=255)
                                                          ccaccaGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGa                                                                                                                         >  2:743934/1‑114 (MQ=255)
                                                              cagcagcagCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCa                                                                                 >  1:440938/1‑150 (MQ=255)
                                                                   gcagcGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTccc                                                                            <  1:1416001/150‑1 (MQ=255)
                                                                     agcGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCtt                                                                          <  2:409614/150‑1 (MQ=255)
                                                                      gcGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGC‑GGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCggg                                                                                                                                              >  2:208125/1‑80 (MQ=255)
                                                                      gcGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGC‑GGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCggg                                                                                                                                              <  1:208125/80‑1 (MQ=255)
                                                                       cGACAGCATGCACCAGCCGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACAGGAGCCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCtttt                                                                        <  2:1447672/150‑1 (MQ=255)
                                                                       cGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCtttt                                                                        <  2:855810/150‑1 (MQ=255)
                                                                          cAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTACACGACATTAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTa                                                                     >  2:1455847/1‑150 (MQ=255)
                                                                            gCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAg                                                                   <  1:979222/150‑1 (MQ=255)
                                                                            gCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAg                                                                   <  1:1464331/150‑1 (MQ=255)
                                                                             cATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAgg                                                                  >  1:737253/1‑150 (MQ=255)
                                                                                gAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAggtgg                                                               <  1:1560016/150‑1 (MQ=255)
                                                                                gAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAggtgg                                                               <  1:472410/150‑1 (MQ=255)
                                                                                         gcggcggGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAggtg                                                                <  1:145141/140‑1 (MQ=255)
                                                                                         gcggcggGTAGGCAGGCGATGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAggtg                                                                >  2:145141/1‑140 (MQ=255)
                                                                                              gggTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGt                                                 <  2:980541/150‑1 (MQ=255)
                                                                                              gggTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGt                                                 <  1:1273538/150‑1 (MQ=255)
                                                                                              gggTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGt                                                 <  1:1045625/150‑1 (MQ=255)
                                                                                                gTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGa                                               <  1:758060/150‑1 (MQ=255)
                                                                                                gTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGa                                               <  1:97478/150‑1 (MQ=255)
                                                                                                     caggcGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTgtagg                                                                                                 <  2:28494/95‑1 (MQ=255)
                                                                                                     caggcGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACATCGTTTGTTGTTgtagg                                                                                                 >  1:28494/1‑95 (MQ=255)
                                                                                                      aggcGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCaa                                         >  1:1156318/1‑150 (MQ=255)
                                                                                                      aggcGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCaa                                         >  2:576770/1‑150 (MQ=255)
                                                                                                          gcTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACggg                                     >  2:686968/1‑150 (MQ=255)
                                                                                                          gcTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACggg                                     <  2:1156318/150‑1 (MQ=255)
                                                                                                               gCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTAtt                                <  2:737253/150‑1 (MQ=255)
                                                                                                                   gggCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGa                            <  1:686968/150‑1 (MQ=255)
                                                                                                                       cgcgTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTttgttgttg                                                                                                     >  1:6628/1‑73 (MQ=255)
                                                                                                                       cgcgTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTttgttgttg                                                                                                     <  2:6628/73‑1 (MQ=255)
                                                                                                                       cgcgTCCAGCGTGTACGACGACGCGGCGCAGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTa                                                                     >  2:960810/1‑105 (MQ=255)
                                                                                                                       cgcgTCCAGCGTGTACGACGACGCGGCGCAGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTa                                                                     >  2:960804/1‑105 (MQ=255)
                                                                                                                       cgcgTCCAGCGTGTACGACGACGCGGCGCAGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTa                                                                     <  1:960804/105‑1 (MQ=255)
                                                                                                                       cgcgTCCAGCGTGTACGACGACGCGGCGCAGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTa                                                                     <  1:960810/105‑1 (MQ=255)
                                                                                                                          gTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGtttt                     >  2:856352/1‑150 (MQ=255)
                                                                                                                                 gtgtACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCAtt              <  1:1472878/150‑1 (MQ=255)
                                                                                                                                  tgtACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTg             <  1:419977/150‑1 (MQ=255)
                                                                                                                                    tACGACGACGCGGCGCGTGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTggg           >  1:500232/1‑149 (MQ=255)
                                                                                                                                      cgacgacgCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTACGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCAt               >  1:1252901/1‑144 (MQ=255)
                                                                                                                                      cgacgacgCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCAt               <  2:1252901/144‑1 (MQ=255)
                                                                                                                                       gacgacgCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTGGAggt        >  2:121050/1‑149 (MQ=255)
                                                                                                                                          gacgCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTGGAGGCggg     >  2:381637/1‑150 (MQ=255)
                                                                                                                                           acgCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTGGAGGCgggg    >  1:1214424/1‑150 (MQ=255)
                                                                                                                                             gcggcgcggGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTGGAGTCGGGGCa  >  2:1367220/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GCCATGAACGGCGCTGCGATGGAAATGTTCACCCGGTCGACGTAGTTGATGATGGTCGCCACCAGCAGCAGCGACAGCATGAACCAGCGGCGGCGGGTAGGCAGGCGCTGGGCGGGGGCCGCGTCCAGCGTGTACGACGACGCGGCGCGGGGGGTAGGCGTGCTCGACATGAGTCGACCTCGTTTGTTGTTGTAGGTAGAGGTTGGGCTGCAGTCCCTTTTCTAAGGTGGTCGTACAACGCGGTGAATGCAACGGGGTATTTAGACCGTTTTTTTCATTGGAGGCGGGGCA  >  NC_002947/1910684‑1910974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑