| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,981,136 | C→A | 10.5% | intergenic (+32/‑268) | rpsA → / → ihfB | 30S ribosomal protein S1/integration host factor subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,981,136 | 0 | C | A | 10.5% | 169.4 / 7.0 | 57 | intergenic (+32/‑268) | rpsA/ihfB | 30S ribosomal protein S1/integration host factor subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (23/28); new base A (3/3); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
GAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGACCATTCTT > NC_002947/1980990‑1981285 | gAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCCAGGGGGGGCAACCATGGAGGACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCggg > 2:1277249/1‑150 (MQ=255) aaaGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGt > 1:1526768/1‑150 (MQ=255) tGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCtttttt > 1:1454807/1‑150 (MQ=255) gAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCttttttt < 2:398769/150‑1 (MQ=255) cgcgAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTcc < 1:667962/150‑1 (MQ=255) aTCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCGAGGGTCGCCCtt > 1:713619/1‑130 (MQ=255) aTCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCGAGGGTCGCCCtt < 2:713619/130‑1 (MQ=255) ccAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGCCCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAggtg < 2:944953/150‑1 (MQ=255) ccAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGGTCCGCGTCGAgggt > 1:808238/1‑148 (MQ=255) ccAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGATTGGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAggtg < 2:220843/150‑1 (MQ=255) tGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGAc > 2:525724/1‑136 (MQ=255) tGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGAc < 1:525724/136‑1 (MQ=255) aaaaaCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGa > 2:1060050/1‑150 (MQ=255) aCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGACGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGc < 2:1495583/150‑1 (MQ=255) cGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCa < 1:682331/150‑1 (MQ=255) aGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGCaa < 1:1645465/150‑1 (MQ=255) tgctgcCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTc > 1:1595636/1‑90 (MQ=255) tgctgcCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTc < 2:1595636/90‑1 (MQ=255) cGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCCCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGc < 2:392528/150‑1 (MQ=255) caccacCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTc < 2:1114899/150‑1 (MQ=255) ccaTGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCGAGGGTCGCCCttt > 1:190067/1‑88 (MQ=255) ccaTGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCGAGGGTCGCCCttt < 2:190067/88‑1 (MQ=255) tGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCC‑TTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCActgctg > 1:183849/1‑126 (MQ=255) tGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCC‑TTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCActgctg < 2:183849/126‑1 (MQ=255) ggCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCa > 2:329849/1‑120 (MQ=255) ggCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCa < 1:329849/120‑1 (MQ=255) tgcgctgcTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAg > 1:1483640/1‑150 (MQ=255) tgctgcGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGt > 1:1099657/1‑150 (MQ=255) gcgcGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTa < 2:1526768/150‑1 (MQ=255) cgAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGa > 2:1043940/1‑150 (MQ=255) gAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAg > 1:1144008/1‑150 (MQ=255) cAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGc < 1:1266342/99‑1 (MQ=255) cAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGc > 2:1266342/1‑99 (MQ=255) cAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGAGGGCACTGCTGGAACACGCCAGCTTCAGCCCGCTCGACCCCGTTGTAGGAGCGGGTTCAccc < 1:1277249/150‑1 (MQ=255) aGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGaa < 2:1099657/150‑1 (MQ=255) cTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGt < 1:432891/135‑1 (MQ=255) cTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGt > 2:432891/1‑135 (MQ=255) gAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGgc < 2:1454807/150‑1 (MQ=255) gtGTTTTATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCAc < 2:997722/149‑1 (MQ=255) tGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAacac < 2:548038/88‑1 (MQ=255) tGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAacac > 1:548038/1‑88 (MQ=255) tAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCActgc > 2:69520/1‑69 (MQ=255) tAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCActgc < 1:69520/69‑1 (MQ=255) aaaGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCg > 1:1014125/1‑150 (MQ=255) aaaGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCg < 2:1483640/150‑1 (MQ=255) aaaGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCCTGAAGCTGGCgtgt < 1:1491810/95‑4 (MQ=255) aaaGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCCTGAAGCTGGCgtgt > 2:1491810/1‑92 (MQ=255) aGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCggg > 1:920479/1‑88 (MQ=255) aGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTgg < 2:920479/88‑1 (MQ=255) gggCGCCCCGCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGGGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTa < 2:257008/150‑1 (MQ=255) gggCGACCCGAGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAg > 1:1561016/1‑102 (MQ=255) gggCGACCCGAGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAg < 2:1561016/102‑1 (MQ=255) ggCGACCCTCGGGTCGCCCTTTTTTTTTCTCCGTGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGtgg > 2:820308/1‑148 (MQ=255) cGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGAc < 1:1060050/150‑1 (MQ=255) gACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGgc < 1:84194/123‑1 (MQ=255) gACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGgc > 2:84194/1‑123 (MQ=255) cccTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAATCGGTAGACCAt > 2:376151/1‑150 (MQ=255) cTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAg > 1:622866/1‑74 (MQ=255) cTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAg < 2:622866/74‑1 (MQ=255) cGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGCAGTAGGCGCCACTGGCCTATCGAACCGGTAGACCATTCtt < 2:808238/150‑1 (MQ=255) cGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGACCATTCtt > 1:1194991/1‑150 (MQ=255) | GAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACACGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTAGACCATTCTT > NC_002947/1980990‑1981285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |