| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,092,425 | T→C | 11.3% | intergenic (+28/+63) | deaD → / ← ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,092,425 | 0 | T | C | 11.3% | 117.4 / 4.3 | 44 | intergenic (+28/+63) | deaD/ygiD | ATP‑dependent DEAD‑box RNA helicase DeaD/4,5‑DOPA‑dioxygenase |
| Reads supporting (aligned to +/- strand): ref base T (19/20); new base C (2/3); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCGACAGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGC > NC_002947/2092279‑2092574 | gcgACAGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAg > 2:1236154/1‑150 (MQ=255) cagcttGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGACCTAAGCCGGGGAtt < 1:1603537/150‑1 (MQ=255) tCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc < 1:65749/150‑1 (MQ=255) ggAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGc > 2:1639390/1‑150 (MQ=255) aaGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGccc < 1:1243394/150‑1 (MQ=255) aCGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc < 2:1223534/135‑1 (MQ=255) aCGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTAc > 1:1223534/1‑135 (MQ=255) ccTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTcgcg > 1:1028856/1‑150 (MQ=255) ctgctgTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCcg > 1:1488217/1‑150 (MQ=255) gctgTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGc > 2:1167625/1‑129 (MQ=255) gctgTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGc < 1:1167625/129‑1 (MQ=255) tgTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCt < 1:166181/119‑1 (MQ=255) tgTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAACCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCt > 2:166181/1‑119 (MQ=255) tCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAaga > 1:1010578/1‑150 (MQ=255) ccAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAagag < 1:1428329/150‑1 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAgg > 1:941088/1‑150 (MQ=255) cAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAgg > 1:378149/1‑150 (MQ=255) tCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCtt > 2:248116/1‑150 (MQ=255) acTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCt > 1:1330252/1‑113 (MQ=255) acTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCt < 2:1330252/113‑1 (MQ=255) acTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGaa > 2:554331/1‑150 (MQ=255) tCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCAAGCCCGCGaaagg < 1:1062968/134‑4 (MQ=255) tCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCAAGCCCGCGaaagg > 2:1062968/1‑131 (MQ=255) gcgTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGa < 1:1158336/150‑1 (MQ=255) gcgTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGa < 1:1639390/150‑1 (MQ=255) cgTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGAt < 1:1362329/150‑1 (MQ=255) tggctggcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAg < 2:378149/150‑1 (MQ=255) tggcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGt < 1:554331/150‑1 (MQ=255) gcAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTcc < 2:1010578/150‑1 (MQ=255) gcagcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATcc < 1:248116/150‑1 (MQ=255) gcTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCa < 2:1236654/150‑1 (MQ=255) tGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAgc > 1:1595324/1‑150 (MQ=255) tGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCCTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAg > 2:618642/1‑135 (MQ=255) tGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCCTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAg < 1:618642/135‑1 (MQ=255) gAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATAACAGGTCTGGCTCTGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAgcg > 2:1379594/1‑150 (MQ=255) ttGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGca < 2:1340513/150‑1 (MQ=255) tCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAg < 1:873050/129‑1 (MQ=255) tCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAg > 2:873050/1‑129 (MQ=255) tCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTAAGTCGCCAACGCGCCCAGGGTAAACCCCTGATGcaccac > 1:84703/1‑150 (MQ=255) aaCGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACtt < 2:1488217/150‑1 (MQ=255) aaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGaa < 2:243392/140‑1 (MQ=255) aaaTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGaa > 1:243392/1‑140 (MQ=255) aTCCCCGACCTAAGCCGGGGATTTTTTTTACCTGTACCGGCCCTCTTCGCGGGCAAGCCCGCGaaa < 1:625159/66‑2 (MQ=9) gCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGATGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAgg > 1:866420/1‑150 (MQ=255) ttAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAgtgg > 1:651553/1‑147 (MQ=255) | GCGACAGCTTCAGCTTGGTCGAGCTGCCGGAAGACGGCCTCGAGAAGCTGCTGTCCAAGCTCAAGGACACTCGCGTGGCTGGCAAGCAGCTGAAGCTGCGTCGCTACCGCGAGGATTGATCCTGGCGTAACGAAAAAATCCCCGGCTTAGGTCGGGGATTTTTTTTACCTGTACCGGCCCT‑TTCGCGGGCTTGCCCGCGAAGAGGCCCTTCAGTCGAACCGATAGATGTCCATCCCCAGCGCGCCCAGGGTAAACCCCTGATGCACCACGCCGAACTTGCTCCCGGCCCCAAGGGC > NC_002947/2092279‑2092574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |