Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,142,379 A→G 5.3% Q691R (CAG→CGG)  PP_1897 → DNA internalization‑related competence protein ComEC/Rec2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,142,3790AG 5.3% 305.1 / 2.5 104Q691R (CAG→CGG) PP_1897DNA internalization‑related competence protein ComEC/Rec2
Reads supporting (aligned to +/- strand):  ref base A (51/46);  new base G (4/2);  total (56/48)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 106 total aligned reads displayed.

GGACACTGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAGGGGAGTGAGGCAGAG  >  NC_002947/2142230‑2142528
                                                                                                                                                     |                                                                                                                                                     
ggACACTGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCCTCATGGCAGCCGCAGTTCGTCCACAGAGGCGTTCGTACGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACCACAGCTTAGGGCACCCGAACGTGCa                                                                                                                                                       >  2:655468/1‑150 (MQ=255)
 gACACTGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAg                                                                                                                                                      <  1:1038927/150‑1 (MQ=255)
  acacTGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAgg                                                                                                                                                     >  2:583816/1‑150 (MQ=255)
     cTGAAGTGCAGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTCCGGGCACCCGCACGTGCAggtgg                                                                                                                                                  <  2:254715/150‑1 (MQ=255)
      tGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAggtggt                                                                                                                                                 >  2:707380/1‑150 (MQ=255)
      tGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACAGCGGCGTTCGCTCTGGCGGCCGCGCCGCGCTGGGTAGTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAggtggt                                                                                                                                                 >  1:1018027/1‑150 (MQ=255)
       gAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTc                                                                                                                                                >  2:241486/1‑150 (MQ=255)
           tGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGc                                                                                                                                            <  1:583816/150‑1 (MQ=255)
              cgcgTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGt                                                                                                                                         >  1:739325/1‑150 (MQ=255)
               gcgTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTa                                                                                                                                        >  2:816125/1‑150 (MQ=255)
                cgTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTAt                                                                                                                                       <  1:241486/150‑1 (MQ=255)
                 gTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATg                                                                                                                                      <  2:739325/150‑1 (MQ=255)
                  tATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATgg                                                                                                                                     >  1:644903/1‑150 (MQ=255)
                  tATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATgg                                                                                                                                     <  2:1579415/150‑1 (MQ=255)
                  tATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGCGCTGTTGACTTCGCGGGGGCGCAACAACAGCTTCGGGGACCCGTTCGTACAGGTGGTCGAGGCGTATgg                                                                                                                                     >  2:192230/1‑150 (MQ=255)
                           gCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCACACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCTGTAGGGGCGGCAAgg                                                                                                                            >  2:529271/1‑150 (MQ=255)
                              gCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGc                                                                                                                                            >  1:1396051/1‑131 (MQ=255)
                              gCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGc                                                                                                                                            <  2:1396051/131‑1 (MQ=255)
                                 ggCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGggtggt                                                                                                                      >  1:1637749/1‑150 (MQ=255)
                                 ggCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGggtggt                                                                                                                      <  2:6519/150‑1 (MQ=255)
                                    gccgcATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGAt                                                                                                                   <  1:1412972/150‑1 (MQ=255)
                                      cgcATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCAGTATGGGCGGCATGGGGTGGTGATGc                                                                                                                 <  1:707380/150‑1 (MQ=255)
                                       gcATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCa                                                                                                                >  1:133212/1‑150 (MQ=255)
                                        catcatGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCAt                                                                                                               <  2:1285659/150‑1 (MQ=255)
                                          tcatGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTTATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGTTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGa                                                                                                             <  1:649593/150‑1 (MQ=255)
                                                    cGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCggag                                                                                                   <  2:126772/150‑1 (MQ=255)
                                                            gTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGc                                                                                           >  2:503017/1‑150 (MQ=255)
                                                             tCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCATGGGGCATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGCGGGGGCa                                                                                          >  1:1206925/1‑150 (MQ=255)
                                                              ccACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCAt                                                                                         >  2:1511253/1‑150 (MQ=255)
                                                               cACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTGCGGGCACCCCCCTGTGATCATGGTGGAGGACGACGTGCGGCATGGGGGGGTGATGCATGATACGGCGGAGGAGGGGGCagt                                                                                        >  1:1012793/1‑148 (MQ=255)
                                                                aCCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTg                                                                                       >  1:1533831/1‑150 (MQ=255)
                                                                        gTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGt                                                                               <  1:1630273/150‑1 (MQ=255)
                                                                         ttCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGGATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTg                                                                              >  1:766718/1‑150 (MQ=255)
                                                                         ttCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTg                                                                              >  2:937056/1‑150 (MQ=255)
                                                                               cGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTgggg                                                                        <  1:1511253/150‑1 (MQ=255)
                                                                                gggCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGggc                                                                       >  2:959799/1‑150 (MQ=255)
                                                                                  gCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATgg                                                                                                                            <  2:297720/95‑1 (MQ=255)
                                                                                  gCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATgg                                                                                                                            >  1:297720/1‑95 (MQ=255)
                                                                                  gCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGggcgg                                                                     <  2:133212/150‑1 (MQ=255)
                                                                                      ccgcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATg                                                                 >  1:617272/1‑150 (MQ=255)
                                                                                      ccgcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATg                                                                 >  2:1463016/1‑150 (MQ=255)
                                                                                       cgcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATgg                                                                >  1:397046/1‑150 (MQ=255)
                                                                                        gcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGa                                                                                                             >  2:957378/1‑104 (MQ=255)
                                                                                        gcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGa                                                                                                             <  1:957378/104‑1 (MQ=255)
                                                                                        gcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               <  1:816125/150‑1 (MQ=255)
                                                                                        gcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               <  1:959799/150‑1 (MQ=255)
                                                                                        gcaccgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               >  2:1272937/1‑150 (MQ=255)
                                                                                          accgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAggg                                                                                              <  1:1438254/117‑1 (MQ=255)
                                                                                          accgcGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAggg                                                                                              >  2:1438254/1‑117 (MQ=255)
                                                                                                 ggggTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAg                                                      <  1:937056/150‑1 (MQ=255)
                                                                                                    gTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAgggg                                                   <  2:617272/150‑1 (MQ=255)
                                                                                                      aTTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGAt                                                                                                                   <  1:1068900/84‑1 (MQ=255)
                                                                                                      aTTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGAt                                                                                                                   >  2:1068900/1‑84 (MQ=255)
                                                                                                       ttGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               <  2:32135/135‑1 (MQ=255)
                                                                                                       ttGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGAATTGCGGTTGGTGCTGGGGCGGCATggg                                                               >  1:32135/1‑135 (MQ=255)
                                                                                                        tGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTa                                               <  2:644903/150‑1 (MQ=255)
                                                                                                         gATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACAGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               <  1:1175220/133‑1 (MQ=255)
                                                                                                         gATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACAGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               >  2:1175220/1‑133 (MQ=255)
                                                                                                          attgctcgGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGGAGGTGGTCGAGGGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAgg                                             <  1:71440/144‑1 (MQ=255)
                                                                                                          aTTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAgg                                             >  1:1444528/1‑150 (MQ=255)
                                                                                                             tCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGc                                                                                                                 <  2:1161899/79‑1 (MQ=255)
                                                                                                             tCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGc                                                                                                                 >  1:1161899/1‑79 (MQ=255)
                                                                                                             tCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCAt                                                                  >  2:79383/1‑126 (MQ=255)
                                                                                                             tCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCAt                                                                  <  1:79383/126‑1 (MQ=255)
                                                                                                                     ccgcAACAGCTGCTTCGCGATCCGGCCGCTGTAGGTGCTCGAGCGGTGTGGGGGGGATGGGGTGTTGATGGGGGAGCCGGCGGTGGGGGGGGCATTGAGGTTGGGGTTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAgcggcg                                  <  2:696207/149‑1 (MQ=255)
                                                                                                                       gcAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGt                                >  1:1221070/1‑150 (MQ=255)
                                                                                                                       gcAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGt                                >  2:1349497/1‑150 (MQ=255)
                                                                                                                        caaaaaCAGCTTCGGGCCCCCGCACGGGCGGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATAGGGGGGAGGGGGGGGAATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGtt                               <  2:405658/150‑1 (MQ=255)
                                                                                                                         aacaacAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCAt                                                                                                               <  1:431056/69‑1 (MQ=255)
                                                                                                                         aacaacAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCAt                                                                                                               >  2:431056/1‑69 (MQ=255)
                                                                                                                         aacaacAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATCAAGGGGAGGAGGGGGCATTGAGACCGGAAGGCCGTCGGCAGCGGGGAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTc                              >  1:366083/1‑150 (MQ=255)
                                                                                                                         aacaacAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTc                              >  1:405658/1‑150 (MQ=255)
                                                                                                                         aacaacAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTc                              >  1:1487431/1‑150 (MQ=255)
                                                                                                                          acaacaGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               >  1:363803/1‑116 (MQ=255)
                                                                                                                          acaacaGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATggg                                                               <  2:363803/116‑1 (MQ=255)
                                                                                                                               ggCTTCGGGCACCCGAAGGGGAGGGGGGTCGGGGGGTAGGGGGGGAAGGGGGGGGGGATGAATGATACGGGGGAGGGGGGGGCATTGGGGTGGGGGCGGGGGGGGAATGGGGTAGTGGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCgt                        <  1:503017/149‑2 (MQ=255)
                                                                                                                               agttctgggCACCCGGACGGGCAGGTGGTCGAGCGGTATGGGCGGCAGGGGGTGGGGATGGAGGATCGGGGGGGGGGGGGGGAATTGGGGTGGGTGCGGGGGGGGCATGGGGTAGGCGAGGGGGTTGGGGGGCAGCGGCGGTTCTGGCgg                        <  2:1444528/144‑1 (MQ=255)
                                                                                                                               aGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGt                                                                               >  1:1226155/1‑95 (MQ=255)
                                                                                                                               aGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGt                                                                               <  2:1226155/95‑1 (MQ=255)
                                                                                                                                  ttCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTg                                                                           >  2:1186770/1‑96 (MQ=255)
                                                                                                                                  ttCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTg                                                                           <  1:1186770/96‑1 (MQ=255)
                                                                                                                                     tggCCCCCGCCGGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGAATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGa                  <  2:654261/149‑1 (MQ=255)
                                                                                                                                     gatctcccGCACGTGCAGGTGGTCGGGCGGTATGGGAGCCGTGGGGTGGTGGTGCATGATACGGGGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGACATGGGGTCGTCGAGGGGGTCAGGGGGCAGCGGCGGTTCTGGCGGGTTCGa                  <  2:358208/145‑1 (MQ=255)
                                                                                                                                     gaccACCTGCACGTGCGGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGa                  >  1:361203/4‑150 (MQ=255)
                                                                                                                                      ggCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCTGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAg                 >  1:128755/1‑150 (MQ=255)
                                                                                                                                      ggCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAg                 >  1:623023/1‑150 (MQ=255)
                                                                                                                                       gCACCGGCACGGGGAGGTGGTCGTGCGGTGTGGGCGGCATGGGGTGGTGATGCTTGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGTTGGTGCGGCATGGGGTAGTCGGGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGacc                <  2:492727/150‑3 (MQ=255)
                                                                                                                                       gCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAga                >  1:654261/1‑149 (MQ=255)
                                                                                                                                        cACCTGCACGTGCGGGTGGTGGAGCGGTATGGGCGGCATGGGGCGGTGCTGCATGATACGGCGGAGGAGTGGGCATCGCGGTTCGTGTGTGCGGGGCATGGGGCAGTCGAGGGAGTCAGGGGACCGCGGGGGTTCTGGCGGGGTTGAggg               >  2:272829/1‑150 (MQ=255)
                                                                                                                                        cACCCGCACGGGGAGGGGGGCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCTGAGGAGGGGGCATTGCGGTGGGTGCTGGGGGGGCATGGGGTAGTCGGGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAggg               <  2:128755/150‑1 (MQ=255)
                                                                                                                                         aCCTGCACGTGCGGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGAAGTCGGGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAgggg              >  2:846450/1‑150 (MQ=255)
                                                                                                                                          cctgCACGTGCGGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCAGGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAGGGGa             >  2:52728/4‑150 (MQ=255)
                                                                                                                                           ccGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATg                                                                 >  1:742953/1‑97 (MQ=255)
                                                                                                                                           ccGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATg                                                                 <  2:742953/97‑1 (MQ=255)
                                                                                                                                           ccGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGACATGGGGGAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAgagatc            >  1:358208/1‑145 (MQ=255)
                                                                                                                                              cccGTGCAGGGGGTCGGGCGGTTTGGGCGGCATGGGGTGGTGAGGCATGATACGGCGGGGGAGGGGGCATTGCGGTTGGTGCGGGGGCGGCAGGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAGGGGAGTga         <  2:397046/148‑1 (MQ=255)
                                                                                                                                                   gCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAGGGGAGTgaggcag    >  1:623568/1‑150 (MQ=255)
                                                                                                                                                    cAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAgggg                                                   >  1:24937/1‑102 (MQ=255)
                                                                                                                                                    cAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAgggg                                                   <  2:24937/102‑1 (MQ=255)
                                                                                                                                                     aGGGGGTGGGGCGGTATGGCGGGCATGGGGGGGTGATGAAGGATACGGGGGGGGAGGGGGCATTGCGGTGGGGGCTGGGGCGGAAGGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAGGGGAGTgaggcagag  <  2:623568/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGACACTGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGTCCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCGAGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGGGTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAGGGGAGTGAGGCAGAG  >  NC_002947/2142230‑2142528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑