| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 202,309 | C→G | 9.6% | V2605V (GTC→GTG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 202,309 | 0 | C | G | 9.6% | 134.4 / 8.2 | 52 | V2605V (GTC→GTG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (28/19); new base G (3/2); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
GCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAA > NC_002947/202163‑202457 | gCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAcc < 2:1084264/150‑1 (MQ=38) aCGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAc > 1:1080792/1‑125 (MQ=33) aCGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAc < 2:1080792/125‑1 (MQ=33) gCTGTGGATGTAGACTGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATAACCTACACCATCAcc > 2:1257720/1‑150 (MQ=17) gCTGTGGATGTAGACGGTCACACCTTCGACAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGCGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCAcc > 1:1460203/1‑150 (MQ=17) tgtgGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCt > 1:622845/1‑150 (MQ=32) ggATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGAc < 1:1625934/150‑1 (MQ=32) gATGTAGACGGTCACACCTTCGAGAACCCGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCa > 2:1161396/1‑142 (MQ=17) gATGTAGACGGTCACACCTTCGAGAACCCGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCa < 1:1161396/142‑1 (MQ=17) tCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACgg > 1:830378/1‑150 (MQ=35) cacCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTgaga > 1:247586/1‑113 (MQ=33) cacCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTgaga < 2:247586/113‑1 (MQ=33) agaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATca > 2:1477171/1‑150 (MQ=35) cTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaacaac < 1:879512/150‑1 (MQ=35) cTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaacaac < 2:1406371/150‑1 (MQ=35) tGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGCTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACATACACCCTGACCCTGACGAACAAAGACGGTCTGCCGATCAACAGCCAGTCgg > 1:385560/1‑150 (MQ=11) ttCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCg > 1:1623006/1‑69 (MQ=37) ttCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCg < 2:1623006/69‑1 (MQ=37) aaGTGACCGACACCCCCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCg > 1:265162/1‑150 (MQ=21) cGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGt > 2:1322527/1‑61 (MQ=37) cGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGt < 1:1322527/61‑1 (MQ=37) cGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTa < 1:737821/72‑1 (MQ=35) cGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTa > 2:737821/1‑72 (MQ=35) cacCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACggtggtgc > 1:413568/1‑144 (MQ=25) ggtCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgt > 1:1056496/3‑150 (MQ=38) ccTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaacaac < 1:647406/109‑1 (MQ=38) ccTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCaacaac > 2:647406/1‑109 (MQ=38) ccTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgt > 2:885985/1‑150 (MQ=38) tCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACACCCACCCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcg > 1:438234/1‑150 (MQ=255) acgaGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTTGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcgtcg > 1:249341/1‑150 (MQ=255) acgaGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcgtcg < 2:1460203/150‑1 (MQ=39) cgaaggggtggcagtcagcttggccaccacttcgGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTa < 2:18095‑M2/83‑1 (MQ=255) cgaaggggtggcagtcagcttggccaccacttcgGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTa > 1:18095‑M2/35‑117 (MQ=255) gaaggggtggcagtcagcttggccaccacttcgGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGa > 2:90759‑M2/34‑110 (MQ=255) gaaggggtggcagtcagcttggccaccacttcgGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGa < 1:90759‑M2/77‑1 (MQ=255) gaaggggtggcagtcagcttggccaccacttcgGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTg > 2:588097‑M2/34‑150 (MQ=255) cGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCTTGTTGGCAGCCAACa < 2:1056496/150‑1 (MQ=255) gAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAg > 2:1242213/1‑150 (MQ=37) aGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGca < 1:1477171/150‑1 (MQ=39) tGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGcacca < 2:265162/150‑1 (MQ=37) gACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGcaccac > 1:236196/1‑150 (MQ=38) tcaccgccTTCGGTCACCGAAGGGGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGt > 1:607606/7‑150 (MQ=12) tcaccgccTTCGGTCACCGAAGG‑GGTGAGATCACCTAAAGCAAAACAATCAACAGTAAAGACAGTCTGCCGAGCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAATAGCACCACCGGtt > 1:30506/7‑150 (MQ=11) ccccTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCCCGCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCgg < 2:310077/150‑1 (MQ=17) cccTTGGGTCACCCAAGGCGGTGAGATCTCCTACACCCTCACCCTGACCAACAAAGACGGTCTGCCGTTCACCAACCACTCGCAGCTGTACTACCAGCTTACCGACGGCACAACCGTAGTCGTGGCTACCAACAGCCCCAGCgccacggc > 2:300385/1‑143 (MQ=11) cccTTCGTTCACCGAAGGCGGTGCGATCACCTACACCACCACCCTGACCAACAAAGACGGTCTGCCGATCAAGATCCACTCGGAGCTGTACTTCAAGATGACCGACTCCACCCCCGGCTTCGGGGCAAGCAACAGCACCACCGGTTCGGc < 2:438234/150‑1 (MQ=11) cccTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGc > 2:469104/1‑150 (MQ=32) cccTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGc < 2:1037125/150‑1 (MQ=32) ttcgGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGc < 2:227773‑M2/58‑1 (MQ=255) ttcgGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGc > 1:227773‑M2/5‑62 (MQ=255) gTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCa > 1:678864/1‑150 (MQ=32) tCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCaa > 2:1472019/1‑150 (MQ=31) | GCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAA > NC_002947/202163‑202457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |