| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 204,976 | G→C | 10.5% | V3494V (GTG→GTC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 204,976 | 0 | G | C | 10.5% | 109.6 / 6.0 | 38 | V3494V (GTG→GTC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (17/17); new base C (2/2); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
CTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCG > NC_002947/204831‑205070 | cTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTGGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCg > 1:530871/1‑150 (MQ=255) gCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAg < 1:1048848/150‑1 (MQ=255) aTCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGt < 1:878322/150‑1 (MQ=255) cAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTga < 1:1595127/150‑1 (MQ=255) gCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTgg > 1:1393953/1‑145 (MQ=11) gCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTgg < 2:1393953/145‑1 (MQ=11) tGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCa > 2:849247/1‑150 (MQ=255) gggTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCAc < 2:1209337/150‑1 (MQ=255) gTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCTCCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACtt > 1:1238904/1‑150 (MQ=255) tATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTa < 2:114042/150‑1 (MQ=255) gtgGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGt > 2:1203215/1‑126 (MQ=255) gtgGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGt < 1:1203215/126‑1 (MQ=255) tgGATGTAGACGGTCGCACCTTCGACAACCTGGAGCTGGGCGGTGCGGCTGCGGTTCAAGTGACCGATAAATGCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGa < 1:365479/150‑1 (MQ=17) gATGTAGACGGTCGCACCTTCGAACACCTGGCGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGCGGGCGATGACATCACTTACACCATTACCCCGAcc > 2:741627/1‑150 (MQ=17) tGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACcaa < 1:1583068/150‑1 (MQ=255) tAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACcaaca > 1:594362/1‑150 (MQ=255) gACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACaaa < 2:635516/150‑1 (MQ=255) cTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGcc > 2:317850/1‑150 (MQ=255) cTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGcc > 1:1631655/1‑150 (MQ=255) ttCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACcaa > 1:551332/1‑134 (MQ=255) ttCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACcaa < 2:551332/134‑1 (MQ=255) gAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGAc < 2:1198468/92‑1 (MQ=255) gAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGAc > 1:1198468/1‑92 (MQ=255) aaaaCCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCACTTCGGTGGCCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCa > 1:1102384/1‑150 (MQ=255) aCCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATa > 1:407377/1‑150 (MQ=255) cTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAAc > 2:85876/1‑150 (MQ=255) cTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTCACCGAAgg < 1:659732/92‑1 (MQ=255) cTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTCACCGAAgg > 2:659732/1‑92 (MQ=255) cTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGt < 2:630191/150‑1 (MQ=255) tGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGAc < 1:317850/150‑1 (MQ=255) gCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGcc > 2:315842/1‑122 (MQ=255) gCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGcc < 1:315842/122‑1 (MQ=255) ttCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCAc > 1:846618/1‑150 (MQ=255) ttCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCAc > 1:1136708/1‑150 (MQ=255) tCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACg < 2:846618/150‑1 (MQ=255) ggTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCt < 1:85876/150‑1 (MQ=40) ttCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGa < 1:1467194/150‑1 (MQ=39) ccatGACGGATACGACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCg > 2:407111/4‑150 (MQ=16) | CTGGCCAGATCAGCCTGGGTATCACGTCTGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGTTGACCGCGACCCCTTCGGTGACCGAAGGCGGTGAGATCACTTACACCATTACCCTGACCAACAAAGACGGCTTGCCGATCAATAACCACAGTGCATTGACCTTCACGCTGAGCG > NC_002947/204831‑205070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |