| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,320,842 | T→G | 7.0% | N148H (AAC→CAC) | PP_2041 ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,320,842 | 0 | T | G | 7.0% | 166.1 / 2.5 | 57 | N148H (AAC→CAC) | PP_2041 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (28/25); new base G (2/2); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
AGGCTACGGGGTGGCATTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAGGCAGTGGCG > NC_002947/2320694‑2320990 | aGGCTACGGGGTGGCATTACCAGGCGGCTCGCTGCGGCTTGAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTg < 2:1198908/150‑1 (MQ=255)aGGCTACGGGGTGGCATTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTg > 2:599206/1‑150 (MQ=255) gggcggCATTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGcaccac < 1:908403/146‑1 (MQ=255) aTTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGaaa < 2:1469900/150‑1 (MQ=255) tACCAGGCGGCTCGCTGCGGCTTCAGGCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGAGCCAATATAGGTCTTCGGAAAACACGGGCTCGCCGACCACATGGACAAGTTCTACGGCGTGGTGCCCAACGCCGaagaa < 1:129152/150‑4 (MQ=255) aCCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATcc > 1:167195/1‑150 (MQ=255) gcTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGcc < 2:1282913/150‑1 (MQ=255) tGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTc > 1:828695/1‑150 (MQ=255) tGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTc < 2:678588/150‑1 (MQ=255) ttCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACGAGTTCTTCGGCGTTGGGCACCACGCAGAAATCCACCTTGCCCAGATcacacgc > 1:1572182/1‑144 (MQ=255) aGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTg > 2:1450296/1‑150 (MQ=255) ccAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGc > 2:1501808/1‑150 (MQ=255) gggCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTcgcg > 1:1039384/1‑150 (MQ=255) tCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAgg < 2:934020/150‑1 (MQ=255) tCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAgg > 1:934020/1‑150 (MQ=255) gTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGcc > 2:962799/1‑150 (MQ=255) tCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCa > 2:1390846/1‑150 (MQ=255) tCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGACTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCATCTTCGCGGCTCAGGccc > 2:419506/1‑149 (MQ=255) cGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCaaa < 1:1390846/150‑1 (MQ=255) cccAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGt < 2:756399/150‑1 (MQ=255) cccAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGt < 1:1501808/150‑1 (MQ=255) ccAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTg > 2:159233/1‑150 (MQ=255) caggcaggCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTTGCCACTTTCGCGGCTCAGGCCATCCCAAACGGTGa > 1:806963/1‑150 (MQ=255) caggcaggCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGa > 2:26548/1‑150 (MQ=255) gcCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAACCGGTGAGCTTGAt > 2:286937/1‑150 (MQ=255) tACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAg < 2:1204116/150‑1 (MQ=255) ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTTTTCGGCGTGGTGCCCAACGCCGaagaa < 2:998266/66‑4 (MQ=21) ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTTTTCGGCGTGGTGCCCAACGCCGaagaa > 1:998266/1‑63 (MQ=18) ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGt < 1:1021236/145‑1 (MQ=255) ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGt > 2:1021236/1‑145 (MQ=255) tCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTgcctgg < 2:167195/150‑1 (MQ=255) cGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTgcctggg > 1:39796/1‑150 (MQ=255) cGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCGGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTgcctggg < 2:1123548/150‑1 (MQ=255) aaaaCACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGcc > 1:1481198/1‑88 (MQ=255) aaaaCACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGcc < 2:1481198/88‑1 (MQ=255) gggCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAat < 1:599206/150‑1 (MQ=255) gCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAatga > 2:1285866/1‑150 (MQ=255) cTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAatgat < 2:39796/150‑1 (MQ=255) tCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAatgatg < 1:962799/150‑1 (MQ=255) cGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATgg < 1:966196/150‑1 (MQ=255) ggCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACATTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGc > 1:369072/1‑150 (MQ=255) cGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCg < 2:1039384/150‑1 (MQ=255) gACCAAATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCgg > 1:606728/1‑150 (MQ=255) aCAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAAc < 1:376316/89‑1 (MQ=255) aCAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAAc > 2:376316/1‑89 (MQ=255) aCAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGt > 1:37524/1‑150 (MQ=255) cAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTg < 1:159233/150‑1 (MQ=255) aaGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCgg < 1:588037/105‑1 (MQ=255) aaGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCgg > 2:588037/1‑105 (MQ=255) gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATTGCCTGGCACCCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATTCCCGGGAAGCGTGCGTGGGCCTGGGTAATGAGGGCCGGCAACAACAGTGCCg > 2:1580924/1‑150 (MQ=255) gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTaa < 2:33654/126‑1 (MQ=255) gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTaa > 1:33654/1‑126 (MQ=255) gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCg > 1:325642/1‑150 (MQ=255) gatttcGGCGTGGTGCCCAACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGa > 1:1114531/4‑150 (MQ=255) tcttcGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAg < 2:812239/150‑1 (MQ=255) cttcGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAgg < 2:606728/150‑1 (MQ=255) tacGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAGGc > 1:714386/3‑150 (MQ=255) ttGGGCACCACGCCGAAATCCACCTTTCCCAGCTTGATCGCTTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACTTTGCCGAGGCAGTGGCg < 2:1572182/150‑1 (MQ=255) | AGGCTACGGGGTGGCATTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAGGCAGTGGCG > NC_002947/2320694‑2320990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |