Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 205,404 T→A 7.7% L3637Q (CTG→CAG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947205,4040TA 7.7% 143.8 / 5.4 52L3637Q (CTG→CAG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base A (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

GAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTG  >  NC_002947/205262‑205550
                                                                                                                                              |                                                                                                                                                  
gaGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTg                                                                                                                                             >  1:1227558/1‑150 (MQ=32)
  gCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCATTTTGCTGCTGGCTCACGACCAGGTCGTGag                                                                                                                                           <  1:862881/150‑2 (MQ=11)
           cccAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCt                                                                                                                                                    <  2:1402344/132‑1 (MQ=33)
           cccAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCt                                                                                                                                                    >  1:1402344/1‑132 (MQ=33)
            ccAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGcaa                                                                                                                                  >  2:1483084/1‑149 (MQ=32)
            ccAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGcaa                                                                                                                                  <  1:1483084/149‑1 (MQ=32)
                  aaCCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGcc                                                                                                                           >  1:216106/1‑150 (MQ=35)
                    cccGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGt                                                                                                                                              <  1:234627/129‑1 (MQ=18)
                    cccGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCAGGTCGt                                                                                                                                              >  2:234627/1‑129 (MQ=18)
                       gggCGGCGGCAGCGCAGGCGACCTGGTGCAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGt                                                                                                                      <  2:216106/150‑1 (MQ=11)
                            gcagcaACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCa                                                                                                                 <  2:927945/150‑1 (MQ=35)
                                 aaCGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAg                                                                                                            <  2:475189/150‑1 (MQ=32)
                                                      gTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCg                                                                                       >  2:812887/1‑150 (MQ=37)
                                                         aCCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGcc                                                                                        <  2:1218547/146‑1 (MQ=39)
                                                         aCCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGcc                                                                                        >  1:1218547/1‑146 (MQ=39)
                                                                 cgccgACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGc                                                                            >  1:1584986/1‑150 (MQ=37)
                                                                        cAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAg                                                                     >  2:259423/1‑150 (MQ=35)
                                                                        cAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAg                                                                     <  1:605776/150‑1 (MQ=35)
                                                                          gACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAggg                                                                   >  1:1270082/1‑150 (MQ=35)
                                                                           aCTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCCCACGACGTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGc                                                                  >  2:926475/1‑150 (MQ=25)
                                                                             ttCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGa                                                                >  1:281716/1‑150 (MQ=38)
                                                                              tCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGAt                                                               <  1:812887/150‑1 (MQ=38)
                                                                                 gTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGAc                                                            <  2:1572147/150‑1 (MQ=38)
                                                                                 gTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGAc                                                            <  2:1227558/150‑1 (MQ=38)
                                                                                     ccGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGGGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCt                                                        >  1:932990/1‑150 (MQ=37)
                                                                                         gaACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGaaa                                                                                                  <  2:9763/104‑1 (MQ=35)
                                                                                         gaACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGaaa                                                                                                  >  1:9763/1‑104 (MQ=35)
                                                                                             gTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGAt                                                <  2:767494/150‑1 (MQ=37)
                                                                                             gTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGAt                                                <  2:281716/150‑1 (MQ=37)
                                                                                             atCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGAt                                                >  2:1127945/2‑150 (MQ=38)
                                                                                              tCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATg                                               >  2:789116/1‑150 (MQ=39)
                                                                                                  aCCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTgg                                           >  2:1613017/1‑150 (MQ=37)
                                                                                                     gACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTa                                                       <  1:11748/135‑1 (MQ=37)
                                                                                                     gACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTa                                                       >  2:11748/1‑135 (MQ=37)
                                                                                                       ccTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGa                                      >  2:1629406/1‑150 (MQ=35)
                                                                                                        cTTCACTGTTCACGTCACACAGCGACTGGCTCACGAAATGGTCGGGAGCCGAAGCAACAACGCCTAGTCCACCATCAAGTCCTGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGAt                                     >  2:430705/1‑150 (MQ=11)
                                                                                                        cTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGAt                                     <  1:1629406/150‑1 (MQ=35)
                                                                                                         ttCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCa                                        >  1:1378785/1‑146 (MQ=35)
                                                                                                         ttCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCa                                        <  2:1378785/146‑1 (MQ=35)
                                                                                                            aCTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGc                                 <  1:1613017/150‑1 (MQ=34)
                                                                                                            aCTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGc                                 <  2:1185393/150‑1 (MQ=34)
                                                                                                               gTTCACGTCAAGCAGCCGCTGACGTACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGCTCACCATCAGGGCCGCCGAAACCAGCGCGCCGTCCACCCACGCTGCACAGTGCCATGACGTCGACAACGATGCTGGCCAGATCAGCCTg                              >  2:382194/1‑150 (MQ=11)
                                                                                                                ttCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTaca                                                                                   <  1:800603/96‑1 (MQ=32)
                                                                                                                ttCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTgg                             <  1:789116/150‑1 (MQ=31)
                                                                                                                ttCACGTCAACCAACCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTaca                                                                                   >  2:800603/1‑96 (MQ=17)
                                                                                                                         aCCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTAt                         <  2:1590920/145‑1 (MQ=32)
                                                                                                                         aCCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTAt                         >  1:1590920/1‑145 (MQ=32)
                                                                                                                                      tCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTc                   >  2:975962/1‑138 (MQ=33)
                                                                                                                                      tCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGCACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTc                   <  1:975962/138‑1 (MQ=18)
                                                                                                                                      tCACGACCAGGTCGTGAGCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAGCCAACGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGc       >  2:557878/1‑150 (MQ=255)
                                                                                                                                       cACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGt             >  2:1270132/1‑143 (MQ=32)
                                                                                                                                       cACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGt             <  1:1270132/143‑1 (MQ=32)
                                                                                                                                        aCGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCa     >  2:1057532/1‑150 (MQ=34)
                                                                                                                                           aCCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGGGAAGCCTTCGCGCCGTACACCGACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTg  >  1:1435637/1‑150 (MQ=11)
                                                                                                                                              |                                                                                                                                                  
GAGCCTGGTACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGTATCAACTCGGCGGTGGATGCCACTG  >  NC_002947/205262‑205550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑