Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,404,130 G→C 7.4% A238A (GCG→GCC)  PP_2106 → ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,404,1300GC 7.4% 167.9 / 4.1 54A238A (GCG→GCC) PP_2106ammonium transporter
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base C (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GGCGGTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGC  >  NC_002947/2403985‑2404279
                                                                                                                                                 |                                                                                                                                                     
ggCGGTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAAAGTCATGGATGCCCACACCTTGCAGGGCGTCAGCGGCCTGGTGGCCATCa                                                                                                                                                   >  2:213030/1‑150 (MQ=255)
       ccGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGtt                                                                                                                                            >  2:205479/1‑150 (MQ=255)
       ccGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGtt                                                                                                                                            <  1:424687/150‑1 (MQ=255)
            aTGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATgg                                                                                                                                       <  1:98242/150‑1 (MQ=255)
                gCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGTCTGGTGGCCATCAACGAGTTGATCGCCac                                                                                                                                   <  1:213030/150‑2 (MQ=255)
                 cGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAtg                                                                                                                                  <  2:728440/150‑1 (MQ=255)
                     tggtggCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGATGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCAtggtgg                                                                                                                              <  1:633573/150‑1 (MQ=255)
                          gCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGc                                                                                                                         >  1:1385095/1‑150 (MQ=255)
                               tGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCATGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCt                                                                                                                    <  1:1468214/150‑1 (MQ=255)
                                    cATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTAAACGTGATGAGTGCCAAGACCTTGCAGGGCGTAAGAGGCCTGGTGGCGATCAACTCGTTGATGGCGATGGTGGGTGGAACGCTGTGAg                                                                                                               >  2:259622/1‑150 (MQ=255)
                                     aTCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGc                                                                                                              >  1:394594/1‑150 (MQ=255)
                                      tCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCg                                                                                                             >  1:1052306/1‑150 (MQ=255)
                                        gAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGtt                                                                                                           >  1:1114048/1‑150 (MQ=255)
                                                 cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                  >  1:1478901/1‑150 (MQ=255)
                                                 cTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCgg                                                                                                  >  2:304022/1‑150 (MQ=255)
                                                       ggCGCTGGGCTCCTGGATCCTCATCATCTGCTGGTTCGTCTTCAACTTCATGAGTGCCCAGACCTTGCAGCGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCTGGCGCaa                                                                                            <  1:259622/150‑1 (MQ=255)
                                                          gcTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGa                                                                                         <  2:1385095/150‑1 (MQ=255)
                                                              ggCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCCACGACCCg                                                                                     >  2:250476/1‑150 (MQ=255)
                                                                    tGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGtt                                                                                                                                            >  1:1532714/1‑89 (MQ=255)
                                                                    tGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGtt                                                                                                                                            <  2:1532714/89‑1 (MQ=255)
                                                                      gATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCt                                                                             <  2:1478901/150‑1 (MQ=255)
                                                                           tcatcaTCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCGACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATa                                                                        >  1:1590682/1‑150 (MQ=255)
                                                                           tcatcaTCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGgc                                                                                                <  2:1263449/126‑1 (MQ=255)
                                                                           tcatcaTCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGgc                                                                                                >  1:1263449/1‑126 (MQ=255)
                                                                             atcatcGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAAc                                                                      <  1:682270/150‑1 (MQ=255)
                                                                                   ggCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTg                                                                                                       >  1:754151/1‑111 (MQ=255)
                                                                                   ggCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTg                                                                                                       <  2:754151/111‑1 (MQ=255)
                                                                                    gCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGc                                                               >  1:886519/1‑150 (MQ=255)
                                                                                     cTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCt                                                              <  2:1590682/150‑1 (MQ=255)
                                                                                        gTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                            <  2:857127/85‑1 (MQ=255)
                                                                                        gTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATggtgggt                                                                                                                            >  1:857127/1‑85 (MQ=255)
                                                                                             gCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGc                                                      <  2:1052306/150‑1 (MQ=255)
                                                                                              cTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGAt                                                                                                                                         >  2:834490/1‑66 (MQ=255)
                                                                                              cTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGAt                                                                                                                                         <  1:834490/66‑1 (MQ=255)
                                                                                               ttCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGtt                                                                                                           <  2:1511985/95‑1 (MQ=255)
                                                                                               ttCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGtt                                                                                                           >  1:1511985/1‑95 (MQ=255)
                                                                                               ttCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtg                                                    >  2:409485/1‑150 (MQ=255)
                                                                                               ttCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCtg                                                    >  1:1566430/1‑150 (MQ=255)
                                                                                               ttCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTAGGTACGCGTCTGTCAGCGTTGCTGGCCGGGCGCAACGACAAGGGCTTTATGATTAATTTTTCGCCGGCCGGGCtg                                                    >  1:1593683/1‑150 (MQ=255)
                                                                                                         tGAGTCCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                          <  1:230227/150‑1 (MQ=255)
                                                                                                                ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAgg                                   >  2:1219189/1‑150 (MQ=255)
                                                                                                                ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAgg                                   >  2:622865/1‑150 (MQ=255)
                                                                                                                ccAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGT‑‑‑‑‑GCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCAAGCTGGTAGGGTTGGTGGCGATTTGCGCAGGTTTCg                              >  2:616977/1‑150 (MQ=255)
                                                                                                                    aCCTTGCAGGGCGGCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTAGTGGCGATTTGCGCAGGTTcc                               >  2:843166/1‑150 (MQ=255)
                                                                                                                          cAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTg                         <  1:205479/150‑1 (MQ=255)
                                                                                                                                     cgatcTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTATACATACAGCCGCGCTTTCCTATCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggt              <  1:1265735/146‑1 (MQ=255)
                                                                                                                                       gCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATACCGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgg            <  1:250476/150‑1 (MQ=255)
                                                                                                                                       gCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCTGATCTGATGCAGCCggtgt            <  2:1593683/150‑2 (MQ=255)
                                                                                                                                       gCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgg            <  2:58938/150‑1 (MQ=255)
                                                                                                                                         cTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGNGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgggt          <  1:1219189/150‑1 (MQ=255)
                                                                                                                                            gtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCggtgagatcg       >  2:1265735/1‑144 (MQ=255)
                                                                                                                                            gtggCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTgcg       >  2:1273892/1‑150 (MQ=255)
                                                                                                                                            atggCCATCAACGAGTTGATCGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                          <  2:1524502/114‑1 (MQ=255)
                                                                                                                                            atggCCATCAACGAGTTGATCGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGAttt                                          >  1:1524502/2‑115 (MQ=255)
                                                                                                                                                 gATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGc  >  2:648358/1‑150 (MQ=255)
                                                                                                                                                 gATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGc  >  1:1260272/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GGCGGTACCGCGATGGGCGTTTGGTGGCTTTTGCGCCATCGAGCATTCCCTTTCTGGCGCTGGGCTCGTGGATCCTCATCATCGGCTGGTTCGGCTTCAACGTCATGAGTGCCCAGACCTTGCAGGGCGTCAGCGGCCTGGTGGCGATCAACTCGTTGATGGCCATGGTGGGTGGCACCCTGTCAGCGTTGCTGGCCGGGCGCAACGACCCGGGCTTTCTGCATAACGGCCCGCTGGCCGGGCTGGTGGCGATTTGCGCAGGTTCCGATCTGATGCACCCGGTGGGTGCGCTCGC  >  NC_002947/2403985‑2404279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑