| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 206,064 | T→A | 8.1% | L3857Q (CTG→CAG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 206,064 | 0 | T | A | 8.1% | 182.2 / 3.3 | 60 | L3857Q (CTG→CAG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (31/24); new base A (2/3); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGAC > NC_002947/205915‑206209 | ggTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCt < 2:675050/150‑1 (MQ=255) cGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGTCCAGGTCGTGa < 2:482811/150‑1 (MQ=255) cGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCAGGTCGTGa < 1:1177871/150‑1 (MQ=255) ttACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGa > 2:1470371/1‑150 (MQ=255) gCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGAAGCGGCTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCAc > 1:1118257/1‑150 (MQ=255) ccGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCAcc < 2:526326/150‑1 (MQ=255) gCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCa > 1:1201944/1‑150 (MQ=255) gAAGGCGACCTGGTCCAGGTCACCATCACAGCCGACCAGACGTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGcc < 2:1118257/150‑1 (MQ=255) ggCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGc < 1:873506/150‑1 (MQ=40) aCCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTGACAC‑TAAACCAGCCGCTGGCTAACCCGATGCT‑GGGACGCCTTAGCAACAAGGCCCAGGTCACCATCAAGGCCGGCgaga > 1:366206/1‑150 (MQ=14) gTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAgc > 1:442513/1‑150 (MQ=255) gTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCa > 2:249435/1‑150 (MQ=255) gTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCAGGTCGTGagc < 2:1598410/99‑3 (MQ=255) gTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCAGGTCGTGagc > 1:1598410/1‑97 (MQ=255) aTCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACAcc > 1:355209/1‑150 (MQ=255) tCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACAccc > 2:600268/1‑150 (MQ=255) acaGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCAc > 1:312063/1‑150 (MQ=255) cGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTgc < 2:731639/150‑1 (MQ=255) gACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCga < 2:59128/122‑1 (MQ=40) gACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCga > 1:59128/1‑122 (MQ=40) gACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAc‑cc > 2:1452330/1‑80 (MQ=255) gACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAc‑cc < 1:1452330/80‑1 (MQ=255) gACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAg > 1:503693/1‑84 (MQ=255) gACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAg < 2:503693/84‑1 (MQ=255) gACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAgg < 1:489853/150‑1 (MQ=255) aCTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGc > 1:726148/1‑150 (MQ=255) tCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAt < 1:232726/150‑1 (MQ=255) gTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGAc < 2:312063/150‑1 (MQ=255) gCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTc < 2:1426953/150‑1 (MQ=255) cGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAgg > 1:1568937/1‑98 (MQ=255) cGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAgg < 2:1568937/98‑1 (MQ=255) gagaACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCgaga > 2:393991/1‑106 (MQ=255) gagaACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCgaga < 1:393991/106‑1 (MQ=255) gTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCa < 2:1008439/77‑1 (MQ=255) gTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCa > 1:1008439/1‑77 (MQ=255) gTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacgac > 2:751816/1‑150 (MQ=255) cAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGAGCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacgacgg > 1:113092/1‑149 (MQ=255) aaCCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTg > 1:735354/1‑150 (MQ=255) gcagcGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTgc < 1:1454951/108‑1 (MQ=255) gcagcGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTgc > 2:1454951/5‑112 (MQ=255) tG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTgg < 2:442513/150‑1 (MQ=39) aCGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCa > 1:1372844/1‑150 (MQ=38) aCGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCa > 1:1589630/1‑150 (MQ=39) gTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATc > 1:155957/1‑150 (MQ=38) gTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATc > 1:1468739/1‑150 (MQ=37) aaaCCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCAc < 2:735354/150‑1 (MQ=37) aaCCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTg < 1:1393511/127‑1 (MQ=35) aaCCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTg > 2:1393511/1‑127 (MQ=37) aCCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGt < 2:1201944/150‑1 (MQ=35) ccAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTc > 1:840531/1‑150 (MQ=35) gCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCtgt < 2:1589630/150‑1 (MQ=38) cTGGCCCACGACCTGGTCGTGCC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCtgtg < 1:1470371/150‑1 (MQ=18) gggtCACGACCAGGTCGTGAG‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGa > 2:1259515/4‑150 (MQ=255) ggCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGa < 2:695228/150‑1 (MQ=38) gCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAAATCAGCCTGGGCATCACGTCTGCTGTGGAt > 2:1046847/1‑150 (MQ=38) cTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGGCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGACCAGCCTGGGCATCACGTCTGCTGTGGATg < 2:510034/150‑1 (MQ=255) tCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGt > 2:615207/1‑150 (MQ=35) aCGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAg > 2:1323792/1‑150 (MQ=35) gACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAg > 1:1516082/1‑148 (MQ=35) gACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAg < 2:1516082/148‑1 (MQ=35) gACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGAc > 1:957950/1‑150 (MQ=35) | GGTCAGCACGTCGGTTACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACAGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGATCTTCACCGTG‑CACGTAAACCAGCCGCTGGCTCACGACCTGGTCGTGAC‑CCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCTGCTGTGGATGTAGAC > NC_002947/205915‑206209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |