| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,579,691 | T→C | 6.6% | V353A (GTG→GCG) | PP_2260 → | glycerol‑phosphate ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,579,691 | 0 | T | C | 6.6% | 179.2 / 2.4 | 60 | V353A (GTG→GCG) | PP_2260 | glycerol‑phosphate ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (28/28); new base C (2/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
TGGGTCAGAAACATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGC > NC_002947/2579542‑2579837 | tGGGTCAGAAACATTTATGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGTCGGGCTGTTGGt > 1:737995/1‑150 (MQ=255) ggTCAGAAACATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGc > 2:420721/1‑150 (MQ=255) tCAGAAACATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAg > 2:1015637/1‑150 (MQ=255) tCAGAAACATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAg > 2:1615443/1‑150 (MQ=255) cAGAAACATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAgg < 1:1423656/150‑1 (MQ=255) aGAAACATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCCGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGc > 2:1066202/1‑150 (MQ=255) aaCATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGcccc > 2:1125435/1‑150 (MQ=255) cATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCgg < 1:420721/150‑1 (MQ=255) tttCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCgggg < 2:927272/150‑1 (MQ=255) acgtgcgCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGcc < 2:906856‑M2/148‑3 (MQ=255) gtgCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAg < 1:794478/150‑1 (MQ=255) gcAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCga < 1:1066202/150‑1 (MQ=255) aTACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAgg < 1:654583/150‑1 (MQ=255) ggCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGAt < 1:358513/150‑1 (MQ=255) gcatcttgctgcACCTTCCCGGGGTTCACGAGTACCAGGTCGATACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCACGCCCCGGGGC‑TGCGCCAGGCGAGTAGAGGGTGATGGc < 2:156969/144‑1 (MQ=255) ctgcACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGTTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATcc < 1:1461613/150‑1 (MQ=255) ctgcACCTTCCCGGGGTTCACGAGTACCAGCTGGACACGCCGATACGCGTGCTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCTCCAGGCGAGGAGAGGGTGATGGCCGAAATcc > 2:114740/1‑150 (MQ=255) cGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAgg > 1:103893/1‑123 (MQ=255) cGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAgg < 2:103893/123‑1 (MQ=255) ttCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGaaa < 2:1223044/131‑1 (MQ=255) ttCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGaaa > 1:1223044/1‑131 (MQ=255) gAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGG‑CCCGGGGC‑TGCGCCAGGCGAGGagag > 1:329607/1‑111 (MQ=255) gAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGG‑CCCGGGGC‑TGCGCCAGGCGAGGagag < 2:329607/111‑1 (MQ=255) tACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGtt > 2:1308939/1‑150 (MQ=255) ggTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGcc > 1:726172/1‑114 (MQ=255) ggTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGcc < 2:726172/114‑1 (MQ=255) ggTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAgc < 1:1420463/150‑1 (MQ=255) gTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAgcc > 2:134661/1‑150 (MQ=255) gCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGccgccagccc > 1:901827/1‑150 (MQ=255) gcgTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGa > 2:584593/1‑150 (MQ=255) gcgTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGa > 1:675480/1‑150 (MQ=255) gcgTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGCGCAG‑CCCCGGGGCCTGCAccaacagcccggcgctgtcgaaaa < 1:932346‑M2/97‑25 (MQ=255) gcgTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGCGCAG‑CCCCGGGGCCTGCAccaacagcccggcgctgtcgaaaa > 2:932346‑M2/1‑73 (MQ=255) cgTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAg < 2:737995/150‑1 (MQ=255) gtTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCa > 1:1522459/1‑150 (MQ=255) gtTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCa < 1:1615443/150‑1 (MQ=255) ttCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAg > 1:24356/1‑150 (MQ=255) tCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGa < 1:584593/150‑1 (MQ=255) aTCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAggg > 1:387286/1‑81 (MQ=255) aTCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAggg < 2:387286/81‑1 (MQ=255) cccACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCGGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCCCCAGCCCGCCAGCGAGGCAGACTATg > 2:304438/1‑150 (MQ=255) cGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCt < 2:1315819/150‑1 (MQ=255) cacaAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTgc < 1:1308939/150‑1 (MQ=255) acaAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACa > 1:641391/1‑113 (MQ=255) acaAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACa < 2:641391/113‑1 (MQ=255) acaAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGCGCAG‑CCCCGGGGCCTGCAccaacagcccgg > 2:827363‑M2/1‑55 (MQ=255) acaAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGCGCAG‑CCCCGGGGCCTGCAccaacagcccgg < 1:827363‑M2/67‑13 (MQ=255) caAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTgcac > 1:119762/1‑150 (MQ=255) aaGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTgcacg > 1:871996/1‑150 (MQ=255) ttCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCg > 2:980704/1‑150 (MQ=255) gTTTTCGACAGCGACGGGCTGTTGGTGCAGGCCCCGGGGG‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGAATGCGCCAGCTGGCGCACAGTTACAGCAGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCAAGCACTCGAAc > 2:478972/1‑150 (MQ=255) tttCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACAt < 2:119762/150‑1 (MQ=255) gACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACa < 1:952365/96‑1 (MQ=255) gACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCATGCGCCAGCTGGCGCACAGTTACa > 2:952365/1‑96 (MQ=255) aCAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACAtgtgtg > 1:59736/1‑150 (MQ=255) cAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTgg < 2:675480/150‑1 (MQ=255) aGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGcag > 2:74575/1‑85 (MQ=255) aGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGcag < 1:74575/86‑2 (MQ=255) ggCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGcaggcg > 2:46457/1‑78 (MQ=255) ggCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGcaggcg < 1:46457/82‑5 (MQ=255) tGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGc < 1:906856/150‑1 (MQ=255) | TGGGTCAGAAACATTTCTGCATGTGCGCAACGAATACTGGCGCATGGTGCTGCACCTTCCCGGGGTTCACGAGTACCAGGTGGACACGCCGATACGCGTGTTCATCCCCACGCACAAGCTGTTCGTTTTCGACAGCGCCGGGCTGTTGGTGCAGGCCCCGGGGC‑TGCGCCAGGCGAGGAGAGGGTGATGGCCGAAATCCGCCTGCGCCAGCTGGCGCACAGTTACAGCCGCCAGCCCGCCAGCGAGGCAGACTATGCCCTGCACGCACTCGAACATGTGTGGGAACAGGGCGGTGC > NC_002947/2579542‑2579837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |