Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,633,500	T→G	7.8%	intergenic (+100/‑82)	hupB → / → PP_2304	DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,633,500	0	T	G	7.8%	133.6 / 4.0	51	intergenic (+100/‑82)	hupB/PP_2304	DNA binding regulator subunit beta B/peptidyl‑prolyl cis‑trans isomerase D
Reads supporting (aligned to +/- strand):  ref base T (22/25);  new base G (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

TCCAGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGT  >  NC_002947/2633355‑2633649                                                                                                                                                  |                                                                                                                                                       tCCAGGTTTCAAGGCCCCCAAACCCCTAAAAGACTCCGTCAACTAAGTCGTCTTTCAGCCGGCCCGCGCATGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGa                                                                                                                                                    <  1:1593787/150‑1 (MQ=255)      gTTTCAAGGCCGGCAAAGGCCTTAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATgcgc                                                                                                                                               <  1:1164164/150‑1 (MQ=255)              gCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGGGCc‑tttcttt                                                                                                                                      <  2:475582/150‑1 (MQ=255)               ccGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCc‑ttttctta                                                                                                                                     <  2:827003/150‑6 (MQ=255)                    aaaGGCCGGAAAGACGCCGTCAACTAAGTCGTCTGTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAg                                                                                                                                <  2:740479/150‑1 (MQ=255)                      aGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGa                                                                                                                              >  2:227422/1‑150 (MQ=255)                               aGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCa                                                                                                                     <  2:991720/150‑1 (MQ=255)                                       tCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCcgc                                                                                                             <  2:1199435/150‑1 (MQ=255)                                           tgacggcgtcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGtt                                                                                                         <  1:1167781/144‑1 (MQ=255)                                             aaGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGACTCTACCCACGCTCCGCGGTTGt                                                                                                       <  2:960686/150‑1 (MQ=255)                                                   tcTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGc                                                                                                 >  1:441554/1‑150 (MQ=255)                                                             ggACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAg                                                                                       >  2:902926/1‑150 (MQ=255)                                                                cccGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCg                                                                                    >  1:265015/1‑150 (MQ=255)                                                                 ccGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACACTCCGCGGTTGTCGACGCAGTGGTACAGCCGt                                                                                   >  1:465680/1‑150 (MQ=255)                                                                     cctggcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCc‑tt                                                                                                                                           >  1:776553/1‑90 (MQ=255)                                                                     cctggcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCGAGGATGCGCc‑tt                                                                                                                                           <  2:776553/90‑1 (MQ=255)                                                                      ctggcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTg                                                                              <  2:265015/150‑1 (MQ=255)                                                                        ggcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTggg                                                                            <  2:202469/150‑1 (MQ=255)                                                                         gcctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgggg                                                                           <  1:227422/150‑1 (MQ=255)                                                                           ctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTgg                                                                                            <  1:983159/131‑1 (MQ=255)                                                                           ctggTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TCTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTgg                                                                                            >  2:983159/1‑131 (MQ=255)                                                                                      aCGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCa                                                              >  1:24329/1‑150 (MQ=255)                                                                                              ggCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCa                                                      <  1:180840/150‑1 (MQ=255)                                                                                                 ggggCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAggg                                                   >  1:112305/1‑150 (MQ=255)                                                                                                  gggCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGa                                                  >  2:934650/1‑150 (MQ=255)                                                                                                      gcAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAAt                                              <  2:24329/150‑1 (MQ=255)                                                                                                       cAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgggg                                                                           <  1:1339274/120‑1 (MQ=255)                                                                                                       cAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgggg                                                                           >  2:1339274/1‑120 (MQ=255)                                                                                                        aaaCGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGGAGTGGTACAGCCGTTTATGGTCGACTCATGCTGCAAAATATGAGGGACAATTc                                            >  2:500392/1‑150 (MQ=255)                                                                                                          aCGTTCCCGCCGGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTcac                                          <  2:419315/150‑1 (MQ=255)                                                                                                                        cacGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGcc                            >  2:1307954/1‑150 (MQ=255)                                                                                                                         acGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTATTTTATCAGGATTCTACCCACGCTCCGCGGTTGTAGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCa                           >  1:104352/1‑150 (MQ=255)                                                                                                                            tttGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGa                        >  2:194967/1‑150 (MQ=255)                                                                                                                            tttGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGa                        <  1:194967/150‑1 (MQ=255)                                                                                                                                 tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACaa                                        <  2:1486150/129‑1 (MQ=255)                                                                                                                                 tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACaa                                        >  1:1486150/1‑129 (MQ=255)                                                                                                                                 tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACcatc                   >  2:1219367/1‑150 (MQ=255)                                                                                                                                 tAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCAACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACcatc                   >  1:177742/1‑150 (MQ=255)                                                                                                                                 aaaagaaaGGCGCATCCGAGGATGCGCCTTTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACcat                    >  1:89123/6‑150 (MQ=255)                                                                                                                                        aGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGCCCATCATCGGcc            <  2:112305/150‑1 (MQ=255)                                                                                                                                        aGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGcc            <  1:934650/150‑1 (MQ=255)                                                                                                                                         ggCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGc                                                                      >  2:1601290/1‑91 (MQ=255)                                                                                                                                         ggCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGc                                                                      <  1:1601290/91‑1 (MQ=255)                                                                                                                                          gcgcATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgg                                                                             >  1:1577488/1‑83 (MQ=255)                                                                                                                                          gcgcATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTgg                                                                             <  2:1577488/83‑1 (MQ=255)                                                                                                                                              aTCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTcac                                          >  2:1161597/1‑114 (MQ=255)                                                                                                                                              aTCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTcac                                          <  1:1161597/114‑1 (MQ=255)                                                                                                                                              aTCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGtt                                    <  1:506046/120‑1 (MQ=255)                                                                                                                                              aTCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGtt                                    >  2:506046/1‑120 (MQ=255)                                                                                                                                               tCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTgg                                                                                            >  1:98482/1‑63 (MQ=255)                                                                                                                                               tCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTgg                                                                                            <  2:98482/63‑1 (MQ=255)                                                                                                                                                  tCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCa                                                      <  1:532232/98‑1 (MQ=255)                                                                                                                                                  tCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCa                                                      >  2:532232/1‑98 (MQ=255)                                                                                                                                                  tCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATcgtcgt  <  1:902926/150‑1 (MQ=255)                                                                                                                                                  tCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATcgtcgt  <  2:104352/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                       TCCAGGTTTCAAGGCCGGCAAAGGCCTGAAAGACGCCGTCAACTAAGTCGTCTTTCAGCCGGACCCGGCCTGGCCTGGTCCGAGCACGGTGATGGCGGGGCGCAAACGTTCCCGCCTGACACGTTTGCTAAGAAAAGGCGCATCCTCGGATGCGCC‑TTTCTTTTATCAGGATTCTACCCACGCTCCGCGGTTGTCGACGCAGTGGTACAGCCGTTTCTGGGGGACGCATGCTGCAAAATATCAGGGACAATTCACAAGGTTGGATTGCCAAGACCATCATCGGCCTTATCGTCGT  >  NC_002947/2633355‑2633649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑