| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,669,714 | T→G | 13.6% | intergenic (+38/+37) | prpD → / ← acnB | 2‑methylcitrate dehydratase/aconitate hydratase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,669,714 | 0 | T | G | 13.6% | 136.7 / 17.6 | 59 | intergenic (+38/+37) | prpD/acnB | 2‑methylcitrate dehydratase/aconitate hydratase B |
| Reads supporting (aligned to +/- strand): ref base T (26/25); new base G (4/4); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
ACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTC > NC_002947/2669569‑2669862 | aCCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCgat > 2:309955/1‑148 (MQ=255) cccGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCGCAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTg > 1:1102803/1‑150 (MQ=255) ccGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGc > 2:174447/1‑150 (MQ=255) gCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCg > 2:299367/1‑150 (MQ=255) tGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCGGGGc < 2:113383/150‑1 (MQ=255) cccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCtt > 1:15375/1‑150 (MQ=255) ccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCttt < 2:1032581/150‑1 (MQ=255) ccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCGGGGCttt < 1:309955/150‑1 (MQ=255) agcagGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTa > 2:1109899/1‑150 (MQ=255) tttCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGc < 1:1000350/150‑1 (MQ=255) gAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTAc < 1:174447/150‑1 (MQ=255) aaCTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTCCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGACTTTGTTTACAACCTGTAACTCAGCTTACg > 1:1569678/1‑150 (MQ=255) aCTGTGCAAGCACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGc < 1:244296/150‑1 (MQ=255) tgtgCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCt > 1:914205/1‑150 (MQ=255) aaGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGGTTGTGATATGAAGCGGCCGGCACTTTCCCTGAGATCAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTg < 1:283109/150‑1 (MQ=255) ggAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGtt < 2:1068766/150‑1 (MQ=255) ggCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCt < 2:491180/150‑1 (MQ=255) ggCACGGCTGTGCAAAGGTTCGTGGACTTGTTTGTGGTATCAGGCGTCCGGCACATGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCt < 2:15375/150‑1 (MQ=255) gCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTg < 1:819193/150‑1 (MQ=255) gCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCgggg < 2:650671/90‑1 (MQ=255) gCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCgggg > 1:650671/1‑90 (MQ=255) ggCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGc > 1:524054/1‑150 (MQ=255) acaGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCggg < 2:437709/77‑1 (MQ=255) acaGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCggg > 1:437709/1‑77 (MQ=255) gTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTc > 2:143789/1‑150 (MQ=255) ttCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCg < 2:1102803/150‑1 (MQ=255) tCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCTTTGGCTGCCGCTTCGCGGAACTCgg < 1:299367/150‑1 (MQ=255) gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTc > 1:102298/1‑137 (MQ=255) gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTc < 2:102298/137‑1 (MQ=255) gACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATc > 2:150147/1‑150 (MQ=255) aCTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg < 1:1447116/122‑1 (MQ=255) aCTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg > 2:1447116/1‑122 (MQ=255) cTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGAt > 1:1561837/1‑147 (MQ=255) cTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGAt < 2:1561837/147‑1 (MQ=255) ttgtttgtTATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTgg > 1:1593403/1‑150 (MQ=255) ttgtttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTgg > 2:515879/1‑150 (MQ=255) ttgtttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGACTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTgg < 2:1569678/150‑1 (MQ=255) tgtgATATGAAGCGACCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCgg < 1:795503/57‑1 (MQ=255) tgtgATATGAAGCGACCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCgg > 2:795503/1‑57 (MQ=255) atatGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTc > 1:922394/1‑150 (MQ=255) atGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAg < 2:914205/150‑1 (MQ=255) gCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAaccacc < 2:89338/89‑1 (MQ=255) gCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAaccacc > 1:89338/1‑89 (MQ=255) cggccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCg < 2:524054/150‑1 (MQ=255) gccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTaa > 2:1604767/1‑64 (MQ=255) gccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTaa < 1:1604767/64‑1 (MQ=255) acaGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGt > 1:410279/1‑150 (MQ=255) aGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCg < 1:1109899/150‑1 (MQ=255) ggCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCgg < 1:143789/150‑1 (MQ=255) ccAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGt > 1:79357/1‑120 (MQ=255) ccAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGt < 2:79357/120‑1 (MQ=255) aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGc > 2:1403633/1‑150 (MQ=255) aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGAAGGTCACCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGc > 1:1252684/1‑150 (MQ=255) caaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCg > 1:1386440/1‑128 (MQ=255) caaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCg < 2:1386440/128‑1 (MQ=255) gCCCCGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGa > 1:976363/1‑150 (MQ=255) ccccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAg < 1:1160090/112‑1 (MQ=255) ccccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAg > 2:1160090/1‑112 (MQ=255) aTCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGCTCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGTTCTATGCTCAGGTAGCGGTACACGTCGGCAGCCATGCTGCCGATGTCTTTc > 2:754771/1‑150 (MQ=255) | ACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTC > NC_002947/2669569‑2669862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |