Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,669,717 C→A 11.8% intergenic (+41/+34) prpD → / ← acnB 2‑methylcitrate dehydratase/aconitate hydratase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,669,7170CA11.8% 164.5 / 9.9 59intergenic (+41/+34)prpD/acnB2‑methylcitrate dehydratase/aconitate hydratase B
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base A (3/4);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

CCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTCGCG  >  NC_002947/2669570‑2669865
                                                                                                                                                   |                                                                                                                                                    
cccGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCGCAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTg                                                                                                                                                    >  1:1102803/1‑150 (MQ=255)
 ccGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGc                                                                                                                                                   >  2:174447/1‑150 (MQ=255)
   gCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCg                                                                                                                                                 >  2:299367/1‑150 (MQ=255)
       tGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCGGGGc                                                                                                                                             <  2:113383/150‑1 (MQ=255)
         cccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCtt                                                                                                                                           >  1:15375/1‑150 (MQ=255)
          ccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCttt                                                                                                                                          <  2:1032581/150‑1 (MQ=255)
          ccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCGGGGCttt                                                                                                                                          <  1:309955/150‑1 (MQ=255)
                        agcagGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTa                                                                                                                            >  2:1109899/1‑150 (MQ=255)
                               tttCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGc                                                                                                                     <  1:1000350/150‑1 (MQ=255)
                                   gAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTAc                                                                                                                 <  1:174447/150‑1 (MQ=255)
                                    aaCTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTCCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGACTTTGTTTACAACCTGTAACTCAGCTTACg                                                                                                                >  1:1569678/1‑150 (MQ=255)
                                     aCTGTGCAAGCACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGc                                                                                                               <  1:244296/150‑1 (MQ=255)
                                       tgtgCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCt                                                                                                             >  1:914205/1‑150 (MQ=255)
                                                        aaGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGGTTGTGATATGAAGCGGCCGGCACTTTCCCTGAGATCAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTg                                                                                            <  1:283109/150‑1 (MQ=255)
                                                             ggAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGtt                                                                                       <  2:1068766/150‑1 (MQ=255)
                                                                 ggCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCt                                                                                   <  2:491180/150‑1 (MQ=255)
                                                                 ggCACGGCTGTGCAAAGGTTCGTGGACTTGTTTGTGGTATCAGGCGTCCGGCACATGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCt                                                                                   <  2:15375/150‑1 (MQ=255)
                                                                  gCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTg                                                                                  <  1:819193/150‑1 (MQ=255)
                                                                  gCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCgggg                                                                                                                                              <  2:650671/90‑1 (MQ=255)
                                                                  gCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCgggg                                                                                                                                              >  1:650671/1‑90 (MQ=255)
                                                                      ggCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGc                                                                              >  1:524054/1‑150 (MQ=255)
                                                                              acaGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCggg                                                                                                                                               <  2:437709/77‑1 (MQ=255)
                                                                              acaGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCggg                                                                                                                                               >  1:437709/1‑77 (MQ=255)
                                                                                  gTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTc                                                                  >  2:143789/1‑150 (MQ=255)
                                                                                   ttCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCg                                                                 <  2:1102803/150‑1 (MQ=255)
                                                                                    tCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCTTTGGCTGCCGCTTCGCGGAACTCgg                                                                <  1:299367/150‑1 (MQ=255)
                                                                                      gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTc                                                                           <  2:102298/137‑1 (MQ=255)
                                                                                      gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTc                                                                           >  1:102298/1‑137 (MQ=255)
                                                                                         gACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATc                                                           >  2:150147/1‑150 (MQ=255)
                                                                                          aCTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg                                                                                      <  1:1447116/122‑1 (MQ=255)
                                                                                          aCTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg                                                                                      >  2:1447116/1‑122 (MQ=255)
                                                                                           cTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGAt                                                            <  2:1561837/147‑1 (MQ=255)
                                                                                           cTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGAt                                                            >  1:1561837/1‑147 (MQ=255)
                                                                                            ttgtttgtTATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTgg                                                        >  1:1593403/1‑150 (MQ=255)
                                                                                            ttgtttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTgg                                                        >  2:515879/1‑150 (MQ=255)
                                                                                            ttgtttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGACTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTgg                                                        <  2:1569678/150‑1 (MQ=255)
                                                                                                 tgtgATATGAAGCGACCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCgg                                                                                                                                                <  1:795503/57‑1 (MQ=255)
                                                                                                 tgtgATATGAAGCGACCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCgg                                                                                                                                                >  2:795503/1‑57 (MQ=255)
                                                                                                     atatGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTc                                               >  1:922394/1‑150 (MQ=255)
                                                                                                       atGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAg                                             <  2:914205/150‑1 (MQ=255)
                                                                                                            gCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAaccacc                                                                                                     <  2:89338/89‑1 (MQ=255)
                                                                                                            gCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAaccacc                                                                                                     >  1:89338/1‑89 (MQ=255)
                                                                                                             cggccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCg                                       <  2:524054/150‑1 (MQ=255)
                                                                                                               gccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTaa                                                                                                                           >  2:1604767/1‑64 (MQ=255)
                                                                                                               gccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTaa                                                                                                                           <  1:1604767/64‑1 (MQ=255)
                                                                                                                     acaGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGt                               >  1:410279/1‑150 (MQ=255)
                                                                                                                       aGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCg                             <  1:1109899/150‑1 (MQ=255)
                                                                                                                        ggCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCgg                            <  1:143789/150‑1 (MQ=255)
                                                                                                                           ccAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGt                                                       >  1:79357/1‑120 (MQ=255)
                                                                                                                           ccAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGt                                                       <  2:79357/120‑1 (MQ=255)
                                                                                                                                 aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGc                   >  2:1403633/1‑150 (MQ=255)
                                                                                                                                 aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGAAGGTCACCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGc                   >  1:1252684/1‑150 (MQ=255)
                                                                                                                                   caaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCg                                       >  1:1386440/1‑128 (MQ=255)
                                                                                                                                   caaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCg                                       <  2:1386440/128‑1 (MQ=255)
                                                                                                                                       gCCCCGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGa             >  1:976363/1‑150 (MQ=255)
                                                                                                                                        ccccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAg                                                  <  1:1160090/112‑1 (MQ=255)
                                                                                                                                        ccccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAg                                                  >  2:1160090/1‑112 (MQ=255)
                                                                                                                                               aTCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGCTCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGTTCTATGCTCAGGTAGCGGTACACGTCGGCAGCCATGCTGCCGATGTCTTTc     >  2:754771/1‑150 (MQ=255)
                                                                                                                                                  gCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTcgcg  >  1:306994/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGATGTCTTTCGCG  >  NC_002947/2669570‑2669865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑