Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,730,927 T→C 7.5% F74S (TTC→TCC)  polB → DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,730,9270TC 7.5% 146.6 / 2.8 53F74S (TTC→TCC) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base C (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

TGGCTGGCCACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCA  >  NC_002947/2730779‑2731075
                                                                                                                                                    |                                                                                                                                                    
gggctggcCACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTc                                                                                                                                                     >  2:1127029/2‑150 (MQ=255)
  gctggcCACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGa                                                                                                                                                   <  1:289673/150‑1 (MQ=255)
      gcCACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCAt                                                                                                                                               <  1:1240519/150‑1 (MQ=255)
      gcCACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCAt                                                                                                                                               <  2:143194/150‑1 (MQ=255)
       ccACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATc                                                                                                                                              <  2:847536/150‑1 (MQ=255)
       ccACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATc                                                                                                                                              >  2:77761/1‑150 (MQ=255)
       ccACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATc                                                                                                                                              <  1:1127029/150‑1 (MQ=255)
        cACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCg                                                                                                                                             >  2:24675/1‑150 (MQ=255)
           cGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGccc                                                                                                                                          >  2:605040/1‑150 (MQ=255)
                  gggCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCATGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCACGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTc                                                                                                                                   <  1:1570596/150‑1 (MQ=255)
                     ccGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGc                                                                                                                                <  1:24675/150‑1 (MQ=255)
                       gcgACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGTCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCct                                                                                                                              >  2:1102258/1‑150 (MQ=255)
                       gcgACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGTCct                                                                                                                              >  1:471698/1‑150 (MQ=255)
                         gACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTCCGAGCATCGGGCGATGCTCgaa                                                                                                                                <  2:1159577/146‑3 (MQ=255)
                         gACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTCCGAGCATCGGGCGATGCTCgaa                                                                                                                                >  1:1159577/1‑144 (MQ=255)
                         gACTGTTGCGGCTGGCGGCGCAGGACCGGTTTGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCctct                                                                                                                            <  1:528/150‑1 (MQ=255)
                             gTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATAc                                                                                                                        <  1:748938/150‑1 (MQ=255)
                               tGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGTCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATAccc                                                                                                                      <  1:1102258/150‑1 (MQ=255)
                                        cggcgCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTAAAGCCACTGCGCCTGAAGGACTTCTAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATc                                                                                                             <  1:446926/150‑1 (MQ=255)
                                            gcAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCa                                                                                                         >  2:531577/1‑150 (MQ=255)
                                                      gTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCaa                                                                                               >  1:146552/1‑150 (MQ=255)
                                                      gTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCaa                                                                                               >  1:166495/1‑150 (MQ=255)
                                                        ggCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACt                                                                                             <  2:1480374/150‑1 (MQ=255)
                                                           tttCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTggg                                                                                          >  2:449420/1‑149 (MQ=255)
                                                           tttCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGa                                                                                          >  1:755902/1‑150 (MQ=255)
                                                           tttCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGa                                                                                          >  2:1399744/1‑150 (MQ=255)
                                                            ttCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGag                                                                                         <  1:449420/150‑1 (MQ=255)
                                                                    tCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGTTCGCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCt                                                                                 >  1:514509/1‑150 (MQ=255)
                                                                             ggCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGc                                                                        <  2:713204/150‑1 (MQ=255)
                                                                              gCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGag                                                                                         <  1:1391655/132‑1 (MQ=255)
                                                                              gCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGag                                                                                         >  2:1391655/1‑132 (MQ=255)
                                                                              gCCGAGCACGCCCGGGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCCCTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGcc                                                                       >  1:292840/1‑150 (MQ=255)
                                                                               ccGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCg                                                                      <  2:755902/150‑1 (MQ=255)
                                                                                cGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGAt                                                                                                   <  2:657419/120‑1 (MQ=255)
                                                                                cGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGAt                                                                                                   >  1:657419/1‑120 (MQ=255)
                                                                                         ccGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACgt                                                            >  2:1560206/1‑150 (MQ=255)
                                                                                                   ctgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGcc                                                  <  1:605040/150‑1 (MQ=255)
                                                                                                   ctgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTCCGAGCATCGGGCGATGCTCgaa                                                                                                                                >  2:142786/1‑70 (MQ=37)
                                                                                                   ctgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTCCGAGCATCGGGCGATGCTCgaa                                                                                                                                <  1:142786/72‑3 (MQ=37)
                                                                                                    tgGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTACCGAAGTGTTCGAGGCCg                                                 >  2:913163/1‑150 (MQ=255)
                                                                                                         aaCGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATc                                            >  1:1604157/1‑150 (MQ=255)
                                                                                                           cGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCg                                          <  2:146552/150‑1 (MQ=255)
                                                                                                                 cGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGGGCGATGCTCgaa                                                                                                                                <  1:817477/58‑3 (MQ=37)
                                                                                                                 cGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGGGCGATGCTCgaa                                                                                                                                >  2:817477/1‑56 (MQ=37)
                                                                                                                       gAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACgtgt                                                          <  2:678765/122‑1 (MQ=255)
                                                                                                                       gAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACgtgt                                                          >  1:678765/1‑122 (MQ=255)
                                                                                                                            tCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGNCCGATATCCGGCCGCCGGAGCGTTAcc                         <  1:1560206/150‑1 (MQ=255)
                                                                                                                              aaGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACgt                                                            >  1:718744/1‑113 (MQ=255)
                                                                                                                              aaGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACgt                                                            <  2:718744/113‑1 (MQ=255)
                                                                                                                                     tGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGa                                                               <  2:126374/103‑1 (MQ=255)
                                                                                                                                     tGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGa                                                               >  1:126374/1‑103 (MQ=255)
                                                                                                                                        gcCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCt             >  1:424169/1‑150 (MQ=255)
                                                                                                                                              aGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTtcatca       <  1:1076479/150‑1 (MQ=255)
                                                                                                                                                   ttCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCa  >  1:1113052/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGCTGGCCACCGATGCCGGGCCGCGACTGTTGCGGCTGGCGGCGCAGGAGTCGGTGGCTTTCATTCCTCAGGCGCAGGCCGAGCACGCCCGTGTGCTGCTGGCCAACGAAACCGGGACGAAGCTCAAGCCACTGCGCCTGAAGGACTTCGAGCATCGCCCGATGCTCGGCCTCTATACCCGCCAGCATCGCCAATTGATGCAACTGGAGCAGCGTCTGCGCACGGCCGGTATCGACGTGTTCGAGGCCGATATCCGGCCGCCGGAGCGTTACCTCATGGAGCGCTTCATCACGGCA  >  NC_002947/2730779‑2731075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑