Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,749,655 G→A 7.1% intergenic (+85/+29) PP_2405 → / ← PP_2406 hypothetical protein/quinate/shikimate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,749,6550GA 7.1% 163.5 / 2.1 56intergenic (+85/+29)PP_2405/PP_2406hypothetical protein/quinate/shikimate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (29/23);  new base A (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GGGGATTATGGTGGGCAGGAACAGGTGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGTTGCTGCCATCCAGCG  >  NC_002947/2749514‑2749796
                                                                                                                                             |                                                                                                                                             
ggggATTATGGTGGGCAGGAACAGGTGATACAGGCATTGCTAAAGTAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCcgcg                                                                                                                                       >  2:56955/1‑150 (MQ=255)
              gCAGGAACAGGTGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACt                                                                                                                         >  2:318863/1‑150 (MQ=255)
                aGGAACAGGTGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACttt                                                                                                                       >  2:851494/1‑150 (MQ=255)
                        gTGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAaca                                                                                                                           >  1:1066094/1‑138 (MQ=255)
                        gTGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAaca                                                                                                                           <  2:1066094/138‑1 (MQ=255)
                        gTGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAg                                                                                                               <  2:1255421/150‑1 (MQ=255)
                        gTGATACAGGCTTTGCTGGAGGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAg                                                                                                               <  2:312962/150‑1 (MQ=255)
                         tGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAgg                                                                                                              <  2:278389/150‑1 (MQ=255)
                                  cTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTgg                                                                                                     <  2:1536746/150‑1 (MQ=255)
                                  cTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTgg                                                                                                     <  1:1050927/150‑1 (MQ=255)
                                          gATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGACGAAGCAAACAATTTTCTATCATGTGAAACTGGCAAAGTgc                                                                                             >  1:28244/1‑150 (MQ=255)
                                             gAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGTGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGcc                                                                                          <  1:1323460/150‑1 (MQ=255)
                                             gAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTgcgat                                                                                          <  1:56955/150‑3 (MQ=255)
                                                    ggTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATcc                                                                                   >  2:1245141/1‑150 (MQ=255)
                                                     gTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCcg                                                                                  >  1:51299/1‑150 (MQ=255)
                                                      tGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCcgc                                                                                 >  2:772695/1‑150 (MQ=255)
                                                       gATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGAGAGTGCAAACAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCcgcg                                                                                >  1:1521664/1‑150 (MQ=255)
                                                                   ttggcttcttcgcgggcacgcccgctcccacaggtactgcgcaaacgttactgattgcactgtccctgtgGGGCATGCCCGCGAAGAGGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCt                                                                    >  1:896264‑M2/71‑150 (MQ=255)
                                                                   ccGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGccc                                                                    >  2:839943/1‑149 (MQ=255)
                                                                    cGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGGGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTg                                                                   >  1:232830/1‑150 (MQ=255)
                                                                    cGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAg                                                                                                               <  1:340694/106‑1 (MQ=255)
                                                                    cGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAg                                                                                                               >  2:340694/1‑106 (MQ=255)
                                                                    cGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTg                                                                   <  1:851494/150‑1 (MQ=255)
                                                                        ttcttcgcgggcacgcccgctcccacaggtactgcgcaaacgttactgattgcactgtccctgtgGGGCATGCCCGCGAAGAGGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTtccgc                                                               <  2:896264‑M2/85‑1 (MQ=255)
                                                                         tctTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATc                                                                         <  1:859447/139‑1 (MQ=255)
                                                                         tctTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATc                                                                         >  2:859447/1‑139 (MQ=255)
                                                                         tctTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTgccgcc                                                              >  1:491506/1‑150 (MQ=255)
                                                                          ctTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGccgccc                                                             >  1:1247814/1‑150 (MQ=255)
                                                                            tCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGccgcccgc                                                           >  2:336232/1‑150 (MQ=255)
                                                                              gcgggcacgcccgctcccacaggtactgcgcaaacgttactgattgcactgtccctgtgGGGCATGCCCGCGAAGAAGCCAACACtttt                                                                                                                      >  2:1114211‑M2/60‑89 (MQ=255)
                                                                              gcgggcacgcccgctcccacaggtactgcgcaaacgttactgattgcactgtccctgtgGGGCATGCCCGCGAAGAAGCCAACACtttt                                                                                                                      <  1:1114211‑M2/30‑1 (MQ=255)
                                                                              gcgGGCATGCCCCAAAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTg                                                         <  1:466425/150‑1 (MQ=255)
                                                                                      gCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGaaa                                                                                                         >  1:176851/1‑94 (MQ=255)
                                                                                      gCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGaaa                                                                                                         <  2:176851/94‑1 (MQ=255)
                                                                                         ccACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGaa                                              >  2:906017/1‑150 (MQ=255)
                                                                                         ccACAGGGACAGCGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAg                                                                                                               >  1:367659/1‑85 (MQ=255)
                                                                                         ccACAGGGACAGCGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAg                                                                                                               <  2:367659/85‑1 (MQ=255)
                                                                                          cacaGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGGGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAAt                                             <  2:232830/150‑1 (MQ=255)
                                                                                          cacaGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAAt                                             <  1:1245141/150‑1 (MQ=255)
                                                                                          cacaGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAAt                                             >  2:33951/1‑150 (MQ=255)
                                                                                            caGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGc                                           <  1:318863/150‑1 (MQ=255)
                                                                                                        aaTCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTg                               >  1:370799/1‑150 (MQ=255)
                                                                                                          tCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGa                             >  2:608180/1‑150 (MQ=255)
                                                                                                           cAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGaa                            <  1:613237/150‑1 (MQ=255)
                                                                                                             gTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGGAAAGGGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAaca                          >  2:1549379/1‑150 (MQ=255)
                                                                                                             gTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAAc                                                      <  1:78266/122‑1 (MQ=255)
                                                                                                             gTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAAc                                                      >  2:78266/1‑122 (MQ=255)
                                                                                                               aaCGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACAcc                        >  2:1600147/1‑150 (MQ=255)
                                                                                                                       gggaGTACCTGGGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGtt                <  2:28244/147‑1 (MQ=255)
                                                                                                                              ccTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGTTGCTGCCa         >  1:1019310/1‑150 (MQ=255)
                                                                                                                               cTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGTTGCTGCCAt        <  1:336232/150‑1 (MQ=255)
                                                                                                                               cTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGTTGCTGCCAt        >  1:163743/1‑150 (MQ=255)
                                                                                                                               cTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGTTGCTGCCAt        <  1:1012368/150‑1 (MQ=255)
                                                                                                                                  tgGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGc                                                                                                    <  1:752877/55‑1 (MQ=255)
                                                                                                                                  tgGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGc                                                                                                    >  2:752877/1‑55 (MQ=255)
                                                                                                                                     gAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGTTGCTGCCATCCAGCg  >  1:864040/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                             
GGGGATTATGGTGGGCAGGAACAGGTGATACAGGCTTTGCTGGATGAAAAAGGGTGATTCTCCTGTACCGGCCTCTTCGCGGGCATGCCCCACAGGGACAGTGCAATCAGTAACGTTTGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGAAGCCAACACTTTTCTATCAGGTGAAACTGGCAAAGTGCGCCTGCATCCGCGCCGCATCCGCCTGCCGCCCGCTGAACAGCTCGAATGCCTTCACCGCCTGGAACACCGCCATGTTGCTGCCATCCAGCG  >  NC_002947/2749514‑2749796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑