| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,053,263 | T→C | 6.8% | intergenic (+21/+25) | PP_2664 → / ← agmR | two‑component system sensor histidine kinase/response regulator/glycerol metabolism activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,053,263 | 0 | T | C | 6.8% | 166.0 / 2.2 | 60 | intergenic (+21/+25) | PP_2664/agmR | two‑component system sensor histidine kinase/response regulator/glycerol metabolism activator |
| Reads supporting (aligned to +/- strand): ref base T (32/22); new base C (2/2); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 | |||||||||||
GCAACGATCTCAAGCAGCAGATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGATG > NC_002947/3053127‑3053411 | gCAACGATCTCAAGCAGCAGATCCGAGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCg < 1:821661/150‑1 (MQ=255) cAACGATCTCAAGCAGCAGATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCgg < 1:193713/150‑1 (MQ=255) cAACGATCTCAAGCAGCAGATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCgg > 1:1587175/1‑150 (MQ=255) aaCGATCTCAAGCAGCAGATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGc < 2:158688/150‑1 (MQ=255) tcAAGCAGCAGATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACa > 2:462608/1‑150 (MQ=255) cAAGCAGCAGATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACGCAAGACGGCGATGAGCCATCTGCTGACCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAg < 1:842999/150‑1 (MQ=255) agcagATCCGCGAACTGGGTCACGCGCTGAGGCACAAGCCCGTAAGGCCGCTGAAACTCAAGACGGCGAGATGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCCAGCGGAGCAGCTCaa > 1:934499/1‑150 (MQ=255) gATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGgc > 2:1325877/1‑150 (MQ=255) aTCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGgcg > 1:844851/1‑150 (MQ=255) gcgAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGa < 2:844851/150‑1 (MQ=255) gcgAACTGGGTCACACGCGGCTGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGa < 2:934499/150‑1 (MQ=255) aCTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAAt < 2:1112764/150‑1 (MQ=255) aCTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTGGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAAt < 1:1197396/150‑1 (MQ=255) gggTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAg < 1:1325877/150‑1 (MQ=255) tCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCa < 1:911507/138‑1 (MQ=255) tCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGATCAACGGCGCa > 2:911507/1‑138 (MQ=255) acGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCACAATc > 1:1223146/1‑150 (MQ=255) cacaAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGaa < 2:1130042/100‑1 (MQ=255) cacaAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGaa > 1:1130042/1‑100 (MQ=255) aaGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGc > 1:80188/1‑150 (MQ=255) gACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATg > 2:552570/1‑150 (MQ=255) ccGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGc < 1:462608/150‑1 (MQ=255) cGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAg < 1:1315767/143‑1 (MQ=255) cGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAg > 2:1315767/1‑143 (MQ=255) aTGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTg > 1:253532/1‑150 (MQ=255) aCTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTAccc > 1:481645/1‑150 (MQ=255) tCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGaa < 2:738087/75‑1 (MQ=255) tCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGaa > 1:738087/1‑75 (MQ=255) aaGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGtt > 1:217104/1‑150 (MQ=255) tGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGc > 1:1114188/1‑77 (MQ=255) tGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGc < 2:1114188/77‑1 (MQ=255) tCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTgc > 2:1642710/1‑150 (MQ=255) gCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGat > 1:214715/1‑93 (MQ=255) gCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGat < 2:214715/93‑1 (MQ=255) gTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATc > 1:814035/1‑86 (MQ=255) gTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATc < 2:814035/86‑1 (MQ=255) cTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGaaa > 1:47471/1‑150 (MQ=255) ttGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCa > 1:736537/1‑65 (MQ=255) ttGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCa < 2:736537/65‑1 (MQ=255) cATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAAACGAACAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTgg > 1:1117427/1‑150 (MQ=255) aTAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTggg > 1:1043973/1‑150 (MQ=255) aTAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTggg > 1:1068943/1‑150 (MQ=255) aTAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTggg > 1:308520/1‑150 (MQ=255) gCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCt > 2:574614/1‑150 (MQ=255) ccGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATc > 2:1614906/1‑65 (MQ=255) ccGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATc < 1:1614906/65‑1 (MQ=255) ccGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACggtg < 2:1597121/150‑1 (MQ=255) ccGCTTCGCGGATCAACCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTgc > 2:1564515/1‑118 (MQ=39) ccGCTTCGCGGATCAACCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTgc < 1:1564515/118‑1 (MQ=39) cGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAg > 2:1163656/1‑82 (MQ=255) cGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAg < 1:1163656/82‑1 (MQ=255) gCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACggtggt > 2:481549/1‑150 (MQ=255) gCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACggtggt > 2:674863/1‑150 (MQ=255) gCTTCGCGGATCAACCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCg < 1:1071657/96‑1 (MQ=38) gCTTCGCGGATCAACCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCg > 2:1071657/1‑96 (MQ=38) ttCGCGGGTTGATAAGAGCAGCGGAAGGAAACACACAGCGTCGAAGTTATTGATCAAAGTCGATATCACCAGCGCGGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGttt > 2:1421981/1‑150 (MQ=255) cgcgGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTCCg < 1:1642710/150‑1 (MQ=255) gcgGGTTGATCCGCGAAGCGGCCGGAACAGCCCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGa > 1:904795/1‑105 (MQ=255) gcgGGTTGATCCGCGAAGCGGCCGGAACAGCCCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGa < 2:904795/105‑1 (MQ=255) ggggTGGATACGGGAAGCGGACGGAACAGCTCAACGGCGCAGTTAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCGTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCGCTGACGGGGGTTTCGGc < 2:1223146/149‑1 (MQ=255) ttGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATGGAGTTTGCGCAAAATCGCCGAAACGTCGGCCTTGACGGTGGTTTCGGCTATg < 1:552570/150‑1 (MQ=255) | GCAACGATCTCAAGCAGCAGATCCGCGAACTGGGTCACACGCTGATGCACAAGCCCGTAAGACCGATGAAACTCAAGACGGCGATGAGCCATCTGCTGGCCAGTGGCTTGGCATAGCGGTTGGCCGCTTCGCGGGTTGATCCGCGAAGCGGCCGGAACAGCTCAACGGCGCAGATAATCAGCAAAATCGATATCGCCAGCACTGAGGATGGCTTGTACCCGGTTGTGCACATTGAGTTTGCGCAAAATCGCCGAAACGTGGGCCTTGACGGTGGTTTCGGCGATG > NC_002947/3053127‑3053411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |