Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,064,681 G→C 7.3% S169S (TCG→TCC) ‡ PP_2676 → substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,064,6810GC 7.3% 171.0 / 4.8 55S169S (TCG→TCC) ‡PP_2676substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base C (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

ACCTGATGATGCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTGCAGCAGGCTTTCGCTGCCATG  >  NC_002947/3064536‑3064828
                                                                                                                                                 |                                                                                                                                                   
aCCTGATGATGCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTgg                                                                                                                                                 >  2:966389/1‑150 (MQ=255)
 ccTGATGATGCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGc                                                                                                                                                >  1:453326/1‑150 (MQ=255)
   tgatgaTGCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCACCGAGGCCa                                                                                                                                              >  1:698391/1‑150 (MQ=255)
   tgatgaTGCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAAAGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCa                                                                                                                                              >  1:61498/1‑150 (MQ=255)
    gatgatGCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAg                                                                                                                                             >  1:363704/1‑150 (MQ=255)
      tgatgCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAgtg                                                                                                                                           <  1:1419325/150‑1 (MQ=255)
         tgCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTcg                                                                                                                                        <  1:1057666/150‑1 (MQ=255)
           cGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCACCGAGGCCAgtcgacgg                                                                                                                                      <  2:698391/150‑7 (MQ=255)
                gCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCCCTGTAGCGGTagt                                                                                                                                 >  2:507440/1‑148 (MQ=255)
                  cTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTcc                                                                                                                               >  1:233859/1‑150 (MQ=255)
                        aTCGCGCCTACGCGCAGCGGCGCACCGACCTGGAGGAGCTGGAAAACGCCCAGGTGTTGATGTTCGGCCCCTATCAGCAGGAGTGTTGGATGGTGGTCTACGATCGTGGTGGTGTGGCCTCGGTTGTCGTTGTCGCGGGATTCCagcagc                                                                                                                         <  2:16564/150‑1 (MQ=255)
                         tCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAgcagca                                                                                                                        >  2:1579813/1‑150 (MQ=255)
                           gcgcCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATc                                                                                                                      <  2:845195/150‑1 (MQ=255)
                              ccTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTa                                                                                                                   <  1:1579813/150‑1 (MQ=255)
                                   gcgcAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCca                                                                                                                              >  1:212180/1‑134 (MQ=255)
                                   gcgcAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCca                                                                                                                              <  2:212180/134‑1 (MQ=255)
                                     gcAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCca                                                                                                                              <  1:153361/132‑1 (MQ=255)
                                     gcAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCca                                                                                                                              >  2:153361/1‑132 (MQ=255)
                                     gcAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCACCGAGGCCAgtcgac                                                                                                                                        <  2:653583/122‑5 (MQ=255)
                                     gcAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCACCGAGGCCAgtcgac                                                                                                                                        >  1:653583/1‑118 (MQ=255)
                                             gcAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTgg                                                                                                    >  1:590248/1‑150 (MQ=255)
                                                aCGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACa                                                                                                 >  1:1114908/1‑150 (MQ=255)
                                                  gAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCgg                                                                                                               <  1:520156/134‑1 (MQ=255)
                                                  gAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCgg                                                                                                               >  2:520156/1‑134 (MQ=255)
                                                    gCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGt                                                                                             >  2:478792/1‑150 (MQ=255)
                                                     cTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTg                                                                                            <  1:278640/150‑1 (MQ=255)
                                                     cTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTGGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTg                                                                                            <  2:488303/150‑1 (MQ=255)
                                                              cTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTc                                                                                   >  1:1290556/1‑150 (MQ=255)
                                                                            ggtggtGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGgt                                                                     <  1:507440/150‑1 (MQ=255)
                                                                                   aTGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATcc                                                                                                                     <  2:611617/95‑1 (MQ=255)
                                                                                   aTGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATcc                                                                                                                     <  2:611533/95‑1 (MQ=255)
                                                                                   aTGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATcc                                                                                                                     >  1:611617/1‑95 (MQ=255)
                                                                                   aTGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATcc                                                                                                                     >  1:611533/1‑95 (MQ=255)
                                                                                    tGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACg                                                             <  2:453326/150‑1 (MQ=255)
                                                                                                   agcagGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGTCATGCTCAGCGCCa                                              >  1:160508/1‑150 (MQ=255)
                                                                                                     cagGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACa                                                                                                 <  1:163575/97‑1 (MQ=255)
                                                                                                     cagGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACa                                                                                                 >  2:163575/1‑97 (MQ=255)
                                                                                                      agGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCCTCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGa                                           <  2:748424/150‑1 (MQ=255)
                                                                                                      agGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGa                                           <  1:966389/150‑1 (MQ=255)
                                                                                                        gAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGAcc                                         <  2:1114908/150‑1 (MQ=255)
                                                                                                           cGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATGGTATGGGGGATGCGTCGGAGTGTTTGCCGTCGGTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCGAGGACccac                                      >  1:529882/1‑150 (MQ=255)
                                                                                                                   ggTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGCCGTTCTACCCGACTTCGGTGTTCAACGGCATGCTCAGCGCCATGACCCACCATTGccc                              <  2:61498/150‑1 (MQ=255)
                                                                                                                       gCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACACCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGc                          >  1:94197/1‑150 (MQ=255)
                                                                                                                        ccTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGc                                                                                               <  1:1536110/80‑1 (MQ=255)
                                                                                                                        ccTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGc                                                                                               >  2:1536110/1‑80 (MQ=255)
                                                                                                                         cTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCg                                                                       <  2:342183/103‑1 (MQ=255)
                                                                                                                         cTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCg                                                                       >  1:342183/1‑103 (MQ=255)
                                                                                                                          tACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTg                       >  1:1459135/1‑150 (MQ=255)
                                                                                                                          tACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTg                       >  1:1232344/1‑150 (MQ=255)
                                                                                                                            cGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTgca                     <  2:1459135/150‑1 (MQ=255)
                                                                                                                                   cGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTGCAGCAgg                <  1:1123107/148‑1 (MQ=255)
                                                                                                                                   cGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTGCAGCAgg                >  2:1123107/1‑148 (MQ=255)
                                                                                                                                          tGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTGCAGCAGGCTTTCGCTg       >  2:442055/1‑150 (MQ=255)
                                                                                                                                             ccTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTGCAGCAGGCTTTCGCTGCCa    <  2:363704/150‑1 (MQ=255)
                                                                                                                                               tCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTGCAGCAGGCTTTCGCTGCCATg  >  1:593466/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACCTGATGATGCGGGTGCCTTACGATCGCGCCTACGCGCAGCGGCGCAACGAGCTGGGGGAGCTGGAAAACGCCCAGGTGGTGATGTTCGGCCCCTATCAGCAGGAGCGTTGGCAGGTGGCCTACGATCGCCGTCGACTGGCCTCGGTGGCCAGTGTCGCGGTATTCCAGCAGCATCCTATCGGGGTCGAGGTGGACAGCGTGCCGTCGTTCTACCTGACATCGGTGTTCAACGGCATGCTCAGCGCCAGGACCCACCATTACCCCGGCGTGCAGCAGGCTTTCGCTGCCATG  >  NC_002947/3064536‑3064828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑