Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,187,643 C→A 7.7% R406R (CGG→AGG)  actP‑II → acetate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,187,6430CA 7.7% 167.8 / 2.8 52R406R (CGG→AGG) actP‑IIacetate permease
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base A (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

CCTGCTGCTGGGTTTCTTGTCTGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTGGTATTGCCGCTTCGGCCAACTTCCCGGTGCTGTTCCTGT  >  NC_002947/3187495‑3187784
                                                                                                                                                      |                                                                                                                                             
ccTGCTGCTGGGTTTCTTGTCTGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCg                                                                                                                                              >  2:788583/1‑150 (MQ=255)
 ctgctgctgGGTTTCTTGTCTGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCgg                                                                                                                                             <  2:129756/150‑1 (MQ=255)
  tgctgctgGGTTTCTTGTCTGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCggg                                                                                                                                            <  2:354880/150‑1 (MQ=255)
     tgctgGGTTTCTTGTCTGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGa                                                                                                                                         <  2:125377/150‑1 (MQ=255)
                ttGTCTGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCt                                                                                                                              >  1:787188/1‑150 (MQ=255)
                     tGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCt                                                                                                                         <  2:1496605/150‑1 (MQ=255)
                     tGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCt                                                                                                                         >  1:1496605/1‑150 (MQ=255)
                     tGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCt                                                                                                                         >  2:1483791/1‑150 (MQ=255)
                       cAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGa                                                                                                                       <  2:1289756/150‑1 (MQ=255)
                            aCCTTCGCCACCATCCTGGCTGTGGTTTCCCGCCAGGCTCGGGCCGGGGCGTCGGCCCTAGTACATGACCTGTGTGTAGCGCGTCGCTTAGGCAGCTCCCG‑‑CAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGTCAACCCTGAGCCtt                                                                                                                  >  1:372912/1‑150 (MQ=255)
                              cTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTgg                                                                                                                >  2:1333286/1‑150 (MQ=255)
                              cTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTgg                                                                                                                >  1:1556996/1‑150 (MQ=255)
                                  gCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCgg                                                                                                                                             >  2:1294513/1‑117 (MQ=255)
                                  gCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCgg                                                                                                                                             <  1:1294513/117‑1 (MQ=255)
                                        aTCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGcc                                                                                                      <  1:304770/150‑1 (MQ=255)
                                         tCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCg                                                                                                                                              >  1:482777/1‑109 (MQ=255)
                                         tCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCg                                                                                                                                              <  2:482777/109‑1 (MQ=255)
                                         tCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTgg                                                                                                                >  2:931174/1‑139 (MQ=255)
                                         tCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTgg                                                                                                                <  1:931174/139‑1 (MQ=255)
                                                tgtgGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCtt                                                                                              <  2:787188/150‑1 (MQ=255)
                                                  tgGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTgg                                                                                            <  1:1483791/150‑1 (MQ=255)
                                                         aGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCt                                                                                     >  2:1469168/1‑150 (MQ=255)
                                                         aGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGAGGGTGACCAAGtt                                                                                                                                  >  1:795764/1‑105 (MQ=255)
                                                         aGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGAGGGTGACCAAGtt                                                                                                                                  <  2:795754/105‑1 (MQ=255)
                                                         aGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGAGGGTGACCAAGtt                                                                                                                                  <  2:795764/105‑1 (MQ=255)
                                                         aGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGAGGGTGACCAAGtt                                                                                                                                  >  1:795754/1‑105 (MQ=255)
                                                           gCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGt                                                                                   >  1:867344/1‑150 (MQ=255)
                                                             cTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTgg                                                                                            >  1:201614/1‑139 (MQ=255)
                                                             cTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTgg                                                                                            <  2:201614/139‑1 (MQ=255)
                                                             cTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATc                                                                                       >  1:617897/1‑144 (MQ=255)
                                                             cTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATc                                                                                       <  2:617897/144‑1 (MQ=255)
                                                             cTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTc                                                                                 >  1:248734/1‑150 (MQ=255)
                                                                  ttttgctgggtCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATTAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGaaaa                                                                            <  2:372912/143‑1 (MQ=255)
                                                                  tctGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGaaaa                                                                            >  1:165451/1‑150 (MQ=255)
                                                                        cGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCaa                                                                      <  1:1333286/150‑1 (MQ=255)
                                                                               tCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCt                                                               >  2:1167160/1‑150 (MQ=255)
                                                                                  gCCATCGCCCATGAACTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTc                                                            >  1:881496/1‑150 (MQ=255)
                                                                                        gCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGtt                                                      <  2:1363163/150‑1 (MQ=255)
                                                                                          ccATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAAc                                                                     <  1:1452927/133‑1 (MQ=255)
                                                                                          ccATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAAc                                                                     >  2:1452927/1‑133 (MQ=255)
                                                                                                     aTG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTg                                         <  2:248734/150‑1 (MQ=255)
                                                                                                     aTG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGACGTAGATGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTg                                         <  1:1319607/150‑1 (MQ=255)
                                                                                                            gggT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGGGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGACATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTGGTATTg                                   >  1:543736/1‑150 (MQ=255)
                                                                                                                  aTCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCttt                                          <  1:1486533/138‑1 (MQ=255)
                                                                                                                  aTCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCttt                                          >  2:1486533/1‑138 (MQ=255)
                                                                                                                    cATGAAGGGCGCGGCCAGCGCGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTGGTATTGCCGCTTc                            <  2:165451/150‑1 (MQ=255)
                                                                                                                     aTGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTg                                                                                                                                          <  2:230840/39‑1 (MQ=255)
                                                                                                                     aTGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTg                                                                                                                                          >  1:230840/1‑39 (MQ=255)
                                                                                                                       gAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTGGTATTGCCGCTTCGGc                         <  2:867344/150‑1 (MQ=255)
                                                                                                                                          gcgcAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGc                                              >  2:387902/1‑110 (MQ=255)
                                                                                                                                          gcgcAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGc                                              <  1:387902/110‑1 (MQ=255)
                                                                                                                                              aGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTGGTATTGCCGCTTCGGCCAACTTCCCGGTGCTGTTCCTGt  <  2:1556996/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                             
CCTGCTGCTGGGTTTCTTGTCTGCAGTGACCTTCGCCACCATCCTGGCTGTGGTTTCAGGCCTGGCTCTGGCCGGGGCGTCCGCCATCGCCCATGACCTGTATG‑CGCGGGT‑GATCATGAAGGGCGCGGCCAGCGAGGCGCAGGAGCTGCGGGTGACCAAGTTGGCTACCCTGAGCCTTGGGTTGGTGGCCATAGCCTTGGGCATCCTGTTCGAAAATATCAACGTGGCTTTCATGGTTGCGCTGGCCTTTGGTATTGCCGCTTCGGCCAACTTCCCGGTGCTGTTCCTGT  >  NC_002947/3187495‑3187784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑