Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,198,945 A→T 7.0% intergenic (‑246/+31) ethA ← / ← PP_2806 FAD‑containing monooxygenase EthA/TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,198,9450AT 7.0% 173.1 / 4.5 58intergenic (‑246/+31)ethA/PP_2806FAD‑containing monooxygenase EthA/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (23/30);  new base T (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

CACGTCACTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCA  >  NC_002947/3198799‑3199094
                                                                                                                                                   |                                                                                                                                                     
cacGTCACTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCg                                                                                                                                                    >  2:449968/1‑150 (MQ=255)
   gTCACTGCTATTTCAGGATGATTTGCACCTGTTACTTAGCAGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACg                                                                                                                                                 >  2:479165/1‑150 (MQ=255)
        tGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACgcagca                                                                                                                                            >  1:595734/1‑150 (MQ=255)
         gCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCCGCGACGCAGCAc                                                                                                                                           >  2:180341/1‑150 (MQ=255)
           tATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACgg                                                                                                                                         <  2:595734/150‑1 (MQ=255)
           tATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATACCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCTGCGTCGCTGCACgg                                                                                                                                         <  1:294415/150‑1 (MQ=255)
               tCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGaa                                                                                                                                     <  2:691302/150‑1 (MQ=255)
               tCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGaa                                                                                                                                     <  1:426935/150‑1 (MQ=255)
                 aGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATc                                                                                                                                   <  1:834563/150‑1 (MQ=255)
                 aGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATc                                                                                                                                   >  1:290980/1‑150 (MQ=255)
                  ggATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATcc                                                                                                                                  <  1:1402625/150‑1 (MQ=255)
                   gatgatTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCt                                                                                                                                 >  1:1631934/1‑150 (MQ=255)
                   gatgatTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCt                                                                                                                                 >  1:462109/1‑150 (MQ=255)
                        tttGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTggg                                                                                                                            <  1:1023954/150‑1 (MQ=255)
                               cTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAg                                                                                                                     >  1:1300423/1‑150 (MQ=255)
                                      ttCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTgg                                                                                                              <  2:290980/150‑1 (MQ=255)
                                      ttCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTgg                                                                                                              >  2:1433340/1‑150 (MQ=255)
                                       tCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGc                                                                                                             <  1:1525168/150‑1 (MQ=255)
                                          ccGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGccac                                                                                                          >  1:246017/1‑150 (MQ=255)
                                            gATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGccacca                                                                                                        <  1:479165/150‑1 (MQ=255)
                                                       aGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACt                                                                                             >  2:228614/1‑150 (MQ=255)
                                                         aCTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGc                                                                                           <  2:1484477/150‑1 (MQ=255)
                                                            gggTAAGGCGGGTAGAGAGCTTCACCGGCACGTTGGAAGGGGGGGCGCTGCGGCTTCTAGAGCGGGAGGGGGAGGGGTTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGGGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCt                                                                                         <  2:287713/150‑1 (MQ=255)
                                                              gTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCtgt                                                                                                                               >  1:811917/1‑109 (MQ=255)
                                                              gTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCtgt                                                                                                                               <  2:811917/109‑1 (MQ=255)
                                                               tCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGGGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCa                                                                                     <  2:714438/150‑1 (MQ=255)
                                                                  gTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGc                                                                                  <  2:1631934/150‑1 (MQ=255)
                                                                     tGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCtg                                                                                                                                >  1:420881/1‑101 (MQ=255)
                                                                     tGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCtg                                                                                                                                <  2:420881/101‑1 (MQ=255)
                                                                        tAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGcggtctc                                                                            <  2:246017/150‑1 (MQ=255)
                                                                                 tCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGc                                                                   >  1:346953/1‑150 (MQ=255)
                                                                                 tCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTATCGGc                                                                   >  1:1097409/1‑150 (MQ=255)
                                                                                       gAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCAGGCAGGCGGTCTCGGTCTCGGCCTCCAg                                                             >  1:316638/1‑150 (MQ=255)
                                                                                           gTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt                                                         <  2:1097409/150‑1 (MQ=255)
                                                                                               gAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt                                                         >  1:18735/1‑146 (MQ=255)
                                                                                               gAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt                                                         <  2:18735/146‑1 (MQ=255)
                                                                                                         cgcTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGt                                            <  1:228614/150‑1 (MQ=255)
                                                                                                           cTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGa                                          >  2:1019739/1‑150 (MQ=255)
                                                                                                            tGCGGCTTTCAGAGCCGGAGTGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTGGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGac                                         >  1:190490/1‑149 (MQ=255)
                                                                                                             gCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGc                                        <  1:449968/150‑1 (MQ=255)
                                                                                                             gCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGc                                        <  1:396095/150‑1 (MQ=255)
                                                                                                                 cTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGAc                                    >  2:906667/1‑150 (MQ=255)
                                                                                                                            ggagggggagggGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCTGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCAt                         <  2:1062982/150‑1 (MQ=255)
                                                                                                                                    aggggaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGt                 >  2:97043/1‑150 (MQ=255)
                                                                                                                                     ggggaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGAGAGGCATGCGGTCCGCGTCACGTCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTc                <  1:1019739/150‑1 (MQ=255)
                                                                                                                                         ttCGCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt                                                         >  2:1225151/2‑105 (MQ=39)
                                                                                                                                         ttCGCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt                                                         <  1:1225151/104‑1 (MQ=39)
                                                                                                                                         aTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAAATTGACTCCAAGGCGGGCGGTCTTGGTCTCTGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAAc            <  1:1433340/150‑1 (MQ=255)
                                                                                                                                            gCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGTAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGa         >  2:1064501/1‑150 (MQ=255)
                                                                                                                                             ccGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCAc                                                     >  2:592496/1‑105 (MQ=255)
                                                                                                                                             ccGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCAc                                                     <  1:592496/105‑1 (MQ=255)
                                                                                                                                              cGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGAtt       >  2:1400695/1‑150 (MQ=255)
                                                                                                                                               gTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCGGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGa                             >  1:936335/1‑127 (MQ=255)
                                                                                                                                               gTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGa                             <  2:936335/127‑1 (MQ=255)
                                                                                                                                                 gCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGAt    <  2:1300423/150‑1 (MQ=255)
                                                                                                                                                 gCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGAt    <  2:957777/150‑1 (MQ=255)
                                                                                                                                                  cAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATc   <  1:97043/150‑1 (MQ=255)
                                                                                                                                                  cAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATc   <  1:1400695/150‑1 (MQ=255)
                                                                                                                                                   aGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCa  >  2:539611/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CACGTCACTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAA‑GGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGATCA  >  NC_002947/3198799‑3199094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑