Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,241,021	G→T	5.6%	G225V (GGC→GTC)	PP_2838 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,241,021	0	G	T	5.6%	231.3 / 2.7	72	G225V (GGC→GTC)	PP_2838	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/34);  new base T (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

GGACTGGTCAGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATGGGACCTGGCCACGGCGGTGTA  >  NC_002947/3240872‑3241162                                                                                                                                                      |                                                                                                                                              ggACTGGTCAGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGggcgg                                                                                                                                               <  2:520967/150‑1 (MQ=255)    cTGGTCAGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGgcggcgc                                                                                                                                            <  2:1096612/150‑2 (MQ=255)      ggTCAGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTCACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGAcc                                                                                                                                          <  1:1250245/150‑1 (MQ=255)       gTCAGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCc                                                                                                                                         <  1:776762‑M2/150‑2 (MQ=255)       gTCAGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCc                                                                                                                                         <  1:186215‑M2/150‑2 (MQ=255)          aGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCccct                                                                                                                                      <  2:776641‑M2/150‑5 (MQ=255)            aCTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCccctgc                                                                                                                                    >  2:931429‑M2/1‑144 (MQ=255)                    cTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCTATCGGGCTTCACCATTGCGCTGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCtt                                                                                                                            <  1:1020486/150‑1 (MQ=255)                     tCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTg                                                                                                                           >  2:1543483/1‑150 (MQ=255)                     tCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTg                                                                                                                           >  2:617912/1‑150 (MQ=255)                             cTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTg                                                                                                                   >  1:1361785/1‑150 (MQ=255)                              tGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCGTGATTCGCGTGCCTGGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCGGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGc                                                                                                                  <  1:1195413/150‑1 (MQ=255)                                 gCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAgg                                                                                                               <  2:1266599/150‑1 (MQ=255)                                       tgcGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGc                                                                                                         >  1:871080/1‑150 (MQ=255)                                       tgcGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGc                                                                                                         >  1:466506/1‑150 (MQ=255)                                          gTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAATGGCAGGAGTTCGACCTGGCCATGGCGCTGCACAATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCTACTCTTCAGGTCTTGcaca                                                                                                      >  2:227980/1‑150 (MQ=255)                                          gTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGcaca                                                                                                      >  2:1498114/1‑150 (MQ=255)                                               gAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGcaca                                                                                                      >  1:78663/1‑145 (MQ=255)                                               gAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGcaca                                                                                                      <  2:78663/145‑1 (MQ=255)                                               gAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACagcagc                                                                                                 >  2:1518421/1‑150 (MQ=255)                                               gAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACagcagc                                                                                                 >  2:1094547/1‑150 (MQ=255)                                                  cAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAAc                                                                                              <  2:1355487/150‑1 (MQ=255)                                                     gTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAAcccc                                                                                           >  2:532858/1‑150 (MQ=255)                                                        ccGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCTATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCctgg                                                                                        <  2:186215/150‑1 (MQ=255)                                                        ccGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCctgg                                                                                        <  1:776641/150‑1 (MQ=255)                                                          gTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTTCAGGGCCTGCACATCATCAACCCctggct                                                                                      >  2:427957/1‑150 (MQ=255)                                                            tCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATTTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCctgcctgg                                                                                    <  1:227980/150‑1 (MQ=255)                                                             cAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCtggctggc                                                                                   <  1:427957/150‑1 (MQ=255)                                                                cccTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCtt                                                                                                                            >  1:46887/1‑106 (MQ=255)                                                                cccTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCtt                                                                                                                            <  2:46887/106‑1 (MQ=255)                                                                cccTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGc                                                                                                                          >  1:951578/1‑108 (MQ=255)                                                                cccTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGc                                                                                                                          <  2:951578/108‑1 (MQ=255)                                                                  cTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGGGTCGCCGccctgctccaggcgctcg                                                                                                                        >  1:770525‑M2/1‑90 (MQ=255)                                                                  cTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGGGTCGCCGccctgctccaggcgctcg                                                                                                                        <  2:770525‑M2/108‑19 (MQ=255)                                                                  cTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAAcccc                                                                                           >  1:724708/1‑137 (MQ=255)                                                                  cTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAAcccc                                                                                           <  2:724708/137‑1 (MQ=255)                                                                   tGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGgcg                                                                             >  1:783384/1‑150 (MQ=255)                                                                   tGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGgcg                                                                             >  2:1273155/1‑150 (MQ=255)                                                                   tGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGCGCGGCGACCCCCTGCCGATCCTTGCCACCCTGGAGGGCCTGCGCAGCAGCAACCCCTGGCTGGCCCGgcg                                                                             >  1:771877/1‑150 (MQ=255)                                                                     aTTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGcc                                                                           >  2:1494481/1‑150 (MQ=255)                                                                       tCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCAt                                                                         <  1:1498114/150‑1 (MQ=255)                                                                             gCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCgg                                                                   <  1:532858/150‑1 (MQ=255)                                                                             gCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCACCATGCGCgg                                                                   >  1:1628228/1‑150 (MQ=255)                                                                               cTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGGGTCGCCGccctgctccag                                                                                                                               >  2:342198‑M2/1‑77 (MQ=255)                                                                               cTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGGGTCGCCGccctgctccag                                                                                                                               <  1:342198‑M2/88‑12 (MQ=255)                                                                               cTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATcc                                                                                                                              <  1:1085721/89‑1 (MQ=255)                                                                               cTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATcc                                                                                                                              >  2:1085721/1‑89 (MQ=255)                                                                                   cagcaACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCATGCACAGCAGCAGCCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCaa                                                             <  1:1612100/150‑1 (MQ=255)                                                                                    agcaACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAAt                                                            >  1:1298341/1‑150 (MQ=255)                                                                                    agcaACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAAt                                                            >  1:316014/1‑150 (MQ=255)                                                                                        cgatctcGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCCGCAGGGCCTGCACAGCAGCAGCCCCTGGCTGGGTTGGCGCCATGCGCGGCCGCAATTCGc                                                        <  1:1456265/144‑1 (MQ=255)                                                                                              agctGTCTACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTggg                                                  <  1:401974/147‑1 (MQ=255)                                                                                               gATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCACGGCCACCACAGCAGCAGCCCCTGGCTGGCCCGGCGCCATGCGCGGCCGCAATTCGccagatcg                                                 >  2:1456265/1‑144 (MQ=255)                                                                                                aTGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGcc                                                >  2:1615397/1‑150 (MQ=255)                                                                                                 tGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCca                                               <  2:871080/150‑1 (MQ=255)                                                                                                     gACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCctggc                                                                                       >  2:1573737/1‑106 (MQ=255)                                                                                                     gACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCctggc                                                                                       <  1:1573737/106‑1 (MQ=255)                                                                                                      aCCTTGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCGCGCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACGAt                                          >  1:934004/1‑150 (MQ=255)                                                                                                      aCCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAAt                                          >  1:1414400/1‑150 (MQ=255)                                                                                                              aTGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCgg                                  >  1:809836/1‑150 (MQ=255)                                                                                                                    cTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGt                            <  1:531224/150‑1 (MQ=255)                                                                                                                    cTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGt                            <  2:316014/150‑1 (MQ=255)                                                                                                                    cTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGt                            <  2:1414400/150‑1 (MQ=255)                                                                                                                    cTGCACCATTGCGCAGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGt                            <  2:776762/150‑1 (MQ=255)                                                                                                                      gCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGa                          >  1:1486189/1‑150 (MQ=255)                                                                                                                       cACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTgg                                                   >  2:632610/1‑124 (MQ=255)                                                                                                                       cACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTgg                                                   <  1:632610/124‑1 (MQ=255)                                                                                                                         ccATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATg                       <  1:1543483/150‑1 (MQ=255)                                                                                                                          cATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATgg                      <  1:1094547/150‑1 (MQ=255)                                                                                                                             tGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATGGGAc                   <  1:800101/150‑1 (MQ=255)                                                                                                                                cccGAGGGTCCGGAGCAGGGCGCCGCCCCCCTCCCTATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATGGGACCTg                <  2:1340896/149‑1 (MQ=255)                                                                                                                                        ccTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATGGGACCTGGCCAcggc        <  1:221924/150‑1 (MQ=255)                                                                                                                                           ggAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATGGGACCTGGCCACGGCGgt     <  1:1273155/150‑1 (MQ=255)                                                                                                                                              gCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATGGGACCTGGCCACGGCGGTGTa  >  1:383598/1‑150 (MQ=255)                                                                                                                                                  ggcggcGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAAt                        <  1:951617/124‑1 (MQ=255)                                                                                                                                                  ggcggcGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAAt                        >  2:951617/1‑124 (MQ=255)                                                                                                                                                      |                                                                                                                                              GGACTGGTCAGACTTCGTCCTCGCCGACCTGGGCTTGCTGCGTTACGAGCAGGTGCCGTTCAGCCCTGATTCGCGTGCCTTGCAGCAACGTGACGATGTCGACCTGGCCATGGCGCTGCACCATTGCGCCGAGCGCCTGGAGCAGGGCGGCGACCCCCTGCCGATCCTTGCCACCCTGCAGGGCCTGCACAGCAGCAACCCCTGGCTGGCCCGGCGCCATGCGCGGCTGCAATTCGCCGTGGGCCAACAATGCGAGCGGCTGGGTGAATGGGACCTGGCCACGGCGGTGTA  >  NC_002947/3240872‑3241162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑