| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,247,894 | T→C | 8.8% | V36A (GTG→GCG) | ureA → | urease subunit gamma |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,247,894 | 0 | T | C | 8.8% | 153.8 / 3.5 | 54 | V36A (GTG→GCG) | ureA | urease subunit gamma |
| Reads supporting (aligned to +/- strand): ref base T (27/22); new base C (2/3); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCCTGCCCGCCGCGTATCTGGGCTACCTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGA > NC_002947/3247747‑3248035 | aggccggCCCGCCGCGTATCTGGGCTACCTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTg‑g > 1:745679/7‑150 (MQ=255) gCCTGCCCGCCGCGTATCTGGGCTACCTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCGGAAGCTCAACTACCCGGAGGCCGTG‑gcg > 2:24745/1‑150 (MQ=255) cTGCCCGCCGCGTATCTGGGCTACCTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCt > 2:846725/1‑150 (MQ=255) tCTGGGCTACCTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCgg > 1:528573/1‑150 (MQ=255) ccTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAg < 2:88679/150‑1 (MQ=255) ccTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAg < 2:528573/150‑1 (MQ=255) tGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGgc > 2:1224939/1‑150 (MQ=255) aCCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGAt > 1:742394/1‑150 (MQ=255) cATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATgg > 1:1101598/1‑150 (MQ=255) cccGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑gcgc > 2:664463/1‑101 (MQ=255) cccGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑gcgc < 1:664463/101‑1 (MQ=255) cccGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGc > 1:1470287/1‑150 (MQ=255) cccGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCCGCGCCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGc > 1:198925/1‑150 (MQ=255) gagagaAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAAc > 1:60311/1‑150 (MQ=255) agaAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACtga > 2:1237205/1‑150 (MQ=255) agaAAGACAAACCGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACtga < 2:625944/150‑1 (MQ=255) gaAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGCG‑GCACTGATCAGCGccacg > 1:1007614/1‑103 (MQ=255) gaAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGCG‑GCACTGATCAGCGCcacg < 2:1007614/106‑1 (MQ=255) aGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAg > 1:1623382/1‑150 (MQ=255) aCAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGCG‑GCACTGATCAGCGCCACggc > 1:948098/1‑102 (MQ=255) aCAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGCG‑GCACTGATCAGCGCCACggc < 2:948098/103‑2 (MQ=255) aaCTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTgg < 2:606876/150‑1 (MQ=255) gctgctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCg > 2:1114975/1‑150 (MQ=255) tgctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAg < 1:200718/102‑1 (MQ=255) tgctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAg > 2:200718/1‑102 (MQ=255) gctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGa > 2:1407929/1‑150 (MQ=255) ctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCcg > 2:1586037/1‑107 (MQ=255) ctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCcg < 1:1586037/107‑1 (MQ=255) ctgTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGaa > 2:372538/1‑150 (MQ=255) ttCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGaa < 1:1415092/147‑1 (MQ=255) ttCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGaa > 2:1415092/1‑147 (MQ=255) cTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGa < 2:1269608/150‑1 (MQ=255) tGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGAc > 2:161562/1‑150 (MQ=255) gcgcTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGt < 1:1237205/150‑1 (MQ=255) gctgctGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGa < 2:742394/150‑1 (MQ=255) ctgGCGGAGCGGCGGCTGTCCCGTGGCCTGAAGCTCAACTACCCGGAGGCAGCGAGCGGTGAAGAAACGGGCGGCG‑TCGAAGGCGCCCACGATGGCCACACAGTCGTCGTTATGATGTGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAg < 1:372538/150‑1 (MQ=255) ctgGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAg < 2:745679/150‑1 (MQ=255) ggCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGt < 2:1470287/150‑1 (MQ=255) gcggcTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACtgatga < 1:165997/107‑1 (MQ=255) gcggcTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACtgatga > 2:165997/1‑107 (MQ=255) tGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCg < 1:846725/150‑1 (MQ=255) tGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCg < 1:24745/150‑1 (MQ=255) ggCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGc > 1:813288/1‑150 (MQ=255) ccGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGa > 1:450032/1‑150 (MQ=255) gTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGaaa < 1:1224939/150‑1 (MQ=255) gCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCacga < 2:60311/150‑1 (MQ=255) aaCTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTg < 2:450032/150‑1 (MQ=255) aCTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGaa > 2:256755/1‑74 (MQ=255) aCTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGaa < 1:256755/74‑1 (MQ=255) aCCCGGAGGCCGTG‑GCGCTGATCCGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAgg < 1:1598330/150‑1 (MQ=255) cGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCg > 2:1552690/1‑150 (MQ=255) ggAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGAt < 1:144102/111‑1 (MQ=255) ggAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGAt > 2:144102/1‑111 (MQ=255) ggAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGa > 2:1415986/1‑150 (MQ=255) | CAGCCTGCCCGCCGCGTATCTGGGCTACCTGAGAGAGCACCATGGAGCTTACCCCGAGAGAGAAAGACAAACTGCTGCTGTTCACTGCCGCGCTGCTGGCGGAGCGGCGGCTGGCCCGTGGCCTGAAGCTCAACTACCCGGAGGCCGTG‑GCGCTGATCAGTGCCGCGGTGCTCGAAGGCGCCCGCGATGGCCGCACAGTCGCCGAACTGATGAGCCTGGGGCGTGAAGTGCTGACCCGTGAGCAGGTGATGCCCGGTATCGCTGAAATGCTTCACGACGTGCAGGTCGA > NC_002947/3247747‑3248035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |