Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,317,256	C→G	7.6%	P608P (CCG→CCC)	treSA ←	trehalose synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,317,256	0	C	G	7.6%	191.3 / 4.4	61	P608P (CCG→CCC)	treSA	trehalose synthase A
Reads supporting (aligned to +/- strand):  ref base C (31/25);  new base G (2/3);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCACGC  >  NC_002947/3317113‑3317403                                                                                                                                                |                                                                                                                                                    gTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGgctg                                                                                                                                               <  2:806979/150‑1 (MQ=255)     cGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCGGGgctgctgg                                                                                                                                           <  1:327979/150‑3 (MQ=255)     cGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCGGGgctgctgg                                                                                                                                           <  2:434043/150‑3 (MQ=255)           tCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAg                                                                                                                                     >  1:1241260/1‑150 (MQ=255)            cATGTTGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAgg                                                                                                                                    <  2:1446282/150‑1 (MQ=255)             aTGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGt                                                                                                                                   >  1:938357/1‑150 (MQ=255)              tGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTa                                                                                                                                  >  2:1338457/1‑150 (MQ=255)                  gACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGtgagca                                                                                                                              <  2:938357/150‑6 (MQ=255)                  gACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAg                                                                                                                              >  2:1528913/1‑150 (MQ=255)                       ccGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCt                                                                                                                         >  2:1294390/1‑150 (MQ=255)                       ccGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCt                                                                                                                         >  1:410512/1‑150 (MQ=255)                           cccAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACg                                                                                                                     <  2:1215304/150‑1 (MQ=255)                                        ccGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGc                                                                                                            >  1:1254005/1‑146 (MQ=255)                                        ccGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGc                                                                                                            <  2:1254005/146‑1 (MQ=255)                                              aaCAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAg                                                                                                  <  1:1338457/150‑1 (MQ=255)                                              aaCAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAg                                                                                                  >  1:957621/1‑150 (MQ=255)                                                 agcagTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGa                                                                                                                       >  1:1237271/1‑126 (MQ=255)                                                 agcagTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGa                                                                                                                       <  2:1237271/126‑1 (MQ=255)                                                     gTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGgc                                                                                           <  1:229038/150‑1 (MQ=255)                                                         cTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTa                                                                                                   >  1:1157906/1‑138 (MQ=255)                                                         cTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTa                                                                                                   <  2:1157906/138‑1 (MQ=255)                                                          tCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcaca                                                                                      >  2:1517588/1‑150 (MQ=255)                                                          tCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTAACAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcaca                                                                                      >  1:714918/1‑150 (MQ=255)                                                                  cgcgTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCcagcag                                                                              <  2:957621/150‑1 (MQ=255)                                                                        cTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTttctt                                                                        <  1:1294390/150‑1 (MQ=255)                                                                            ccGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcaca                                                                                      >  1:1344659/1‑132 (MQ=255)                                                                            ccGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcaca                                                                                      <  2:1344659/132‑1 (MQ=255)                                                                             cGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGc                                                                                                                   <  2:852145/102‑1 (MQ=255)                                                                             cGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGc                                                                                                                   >  1:852145/1‑102 (MQ=255)                                                                             cGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGAAGCTTATGGATCATCACCAGCGGCCCCGGGCGGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCt                                                                   >  1:1104511/1‑150 (MQ=255)                                                                              gAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTg                                                                                             >  1:1412395/1‑123 (MQ=255)                                                                              gAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTg                                                                                             <  2:1412395/123‑1 (MQ=255)                                                                                 gTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCa                                                               <  2:1241260/150‑1 (MQ=255)                                                                                      gcgcAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCa                                                                                                                                      >  2:517293/1‑74 (MQ=255)                                                                                      gcgcAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCa                                                                                                                                      <  1:517293/74‑1 (MQ=255)                                                                                         cAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCAcagc                                                                                    >  1:556235/1‑121 (MQ=255)                                                                                         cAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCAcagc                                                                                    <  2:556235/121‑1 (MQ=255)                                                                                           gTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCATCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGaa                                                     >  2:870861/1‑150 (MQ=255)                                                                                               tCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCAc                                                 >  1:473462/1‑150 (MQ=255)                                                                                                 tGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCActc                                               >  2:190535/1‑150 (MQ=255)                                                                                                  ggATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGTAGGCAAATGAATCActct                                              >  1:618892/1‑150 (MQ=255)                                                                                                        cgcgCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCa                                        >  1:1413509/1‑150 (MQ=255)                                                                                                             ccGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGc                                   <  1:190535/150‑1 (MQ=255)                                                                                                             ccGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGc                                   <  2:618892/150‑1 (MQ=255)                                                                                                             ccGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGc                                   >  1:839373/1‑150 (MQ=255)                                                                                                              cGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTAACAACACCTGACGGCTGCAGGGTAGTCCAGATGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGcc                                  >  1:198419/1‑150 (MQ=255)                                                                                                                   ggCAGCTCATGCACCATCACCAGCATCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTcc                             <  1:870861/150‑1 (MQ=255)                                                                                                                    gCAGCTCATGCACCATCACCAGCGCAGCGGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCa                            >  1:1042033/1‑150 (MQ=255)                                                                                                                    gCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCCCAAAACCGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCa                            <  2:1042033/150‑1 (MQ=255)                                                                                                                      aGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCGGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCAGTTTCGTCCAGCTGCCTGTCAAgg                          >  1:437500/1‑150 (MQ=255)                                                                                                                      aGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAgg                          >  2:820266/1‑150 (MQ=255)                                                                                                                      aGCGCATGCACCATCACCAGCAGCCACGGGTTGATCAACTCAGGTACCATTACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAgg                          <  2:1089384/150‑1 (MQ=255)                                                                                                                           aTGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGcc                     >  1:1289043/1‑150 (MQ=255)                                                                                                                           aTGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTGCAGCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGcc                     <  1:739477/150‑1 (MQ=255)                                                                                                                             gCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGt                   >  1:977562/1‑150 (MQ=255)                                                                                                                               aCCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGtat                 <  1:1528913/150‑1 (MQ=255)                                                                                                                                   tCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCTCTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAgcg             >  1:1249608/1‑150 (MQ=255)                                                                                                                                     gtgagcagcCCGGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAg                                                              >  1:904133/4‑99 (MQ=255)                                                                                                                                     gtgagcagcCCGGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAg                                                              <  2:904133/96‑1 (MQ=255)                                                                                                                                       cagcagCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCg         >  1:555698/1‑150 (MQ=255)                                                                                                                                          cagcCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTCCCGTATAGCGCCCGGGc      <  2:839373/150‑1 (MQ=255)                                                                                                                                              cccGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCAcgc  <  2:198419/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    GTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCACGC  >  NC_002947/3317113‑3317403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑