| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,533,413 | A→G | 6.8% | E16E (GAA→GAG) | atoA → | 3‑oxoacid CoA‑transferase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,533,413 | 0 | A | G | 6.8% | 175.0 / 2.6 | 59 | E16E (GAA→GAG) | atoA | 3‑oxoacid CoA‑transferase subunit A |
| Reads supporting (aligned to +/- strand): ref base A (25/30); new base G (2/2); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 | |||||||||||
ATGACGAAAACAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTCT > NC_002947/3533264‑3533556 | aTGACGAAAACAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGaa > 1:799624/1‑150 (MQ=255)aTGACGAAAAAAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGaa > 2:310238/1‑150 (MQ=255) aCAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAGGGCCTGAcc > 2:75692/1‑150 (MQ=255) aTTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGacaa < 1:644415/150‑1 (MQ=255) aTTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGacaa < 2:1329299/150‑1 (MQ=255) aaaTCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGAc < 2:1081623/150‑1 (MQ=255) tCGCTTGCGGTGAAGATGTCTGCACAGGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTg > 1:630529/1‑150 (MQ=255) tCGCTTGCGGTGAAGATGTCTGCACACGATCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTg < 2:1039170/150‑1 (MQ=255) tGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAGGGCCTGACCGACAACATGACGGTACTGGCCgg < 1:75692/150‑1 (MQ=255) cGGTGAAGATGTCTGCACCCGCTCACCGACAGCCTCGTCGGTTAAACACCTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGTTCTAACCGACAACATGACGGTACTGGCCcgtg < 2:474653/150‑1 (MQ=255) aTGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAg < 2:258834/109‑1 (MQ=255) aTGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAg > 1:258834/1‑109 (MQ=255) tGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGcc < 2:1304718/150‑1 (MQ=255) tGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGcc > 2:716975/1‑150 (MQ=255) cacaCGCTCACCGACAGCCTCGTCGGCTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGCCGGTACTGGCCGGTGGTTTCCGCCTGAGCg > 2:157199/1‑150 (MQ=255) cGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACg < 2:1150519/111‑1 (MQ=255) cGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACg > 1:1150519/1‑111 (MQ=255) cAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAAc < 1:716975/150‑1 (MQ=255) ccTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCt < 2:1361120/89‑1 (MQ=255) ccTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCt > 1:1361120/1‑89 (MQ=255) gtcgGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCa < 1:310238/150‑1 (MQ=255) gTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGa < 1:349988/95‑1 (MQ=255) gTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGa > 2:349988/1‑95 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGt < 2:964037/94‑1 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGt > 1:964037/1‑94 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCa > 2:742251/1‑150 (MQ=255) acaacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCAGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCa > 1:1441620/1‑150 (MQ=255) caacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAg > 2:437340/1‑71 (MQ=255) caacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAg < 1:437340/71‑1 (MQ=255) aacTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAg > 1:808799/1‑150 (MQ=255) acTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAgc < 2:808799/150‑1 (MQ=255) tACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGa < 1:1530684/73‑1 (MQ=255) tACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGa > 2:1530684/1‑73 (MQ=255) tACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATccc < 1:212487/122‑1 (MQ=255) tACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATccc > 2:212487/1‑122 (MQ=255) aaaaGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAgcggcgc > 1:66267/1‑150 (MQ=255) cATGGCCGGACTGGACAAGCGCGTTGCNACCTATGAGCAGNCCCTCGNAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCt > 1:1296756/1‑150 (MQ=255) gCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGc < 1:495971/93‑1 (MQ=255) gCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGc > 2:495971/1‑93 (MQ=255) gCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCATCGAAATCAAGCGGCGCGGCGTCaa < 1:851325/140‑1 (MQ=255) gCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCATCGAAATCAAGCGGCGCGGCGTCaa > 2:851325/1‑140 (MQ=255) gCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGAcc < 1:157199/150‑1 (MQ=255) ggACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTg < 1:742251/150‑1 (MQ=255) gACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTc > 2:963345/1‑149 (MQ=255) gACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTc < 1:963345/149‑1 (MQ=255) ccgcgTTGCAACATATGACCAGACACTCGAAGGCCTGACCGACAACATGACGGTACGGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGACCGGACCGTGGTTTCcaacaa > 2:1504430/2‑150 (MQ=255) gCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTgcggc < 2:66267/150‑1 (MQ=255) gCAACCTAGGAGCAGGCCCTCGAAGGCCTGACCGACAACATGCAAGGACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTgcggc < 1:589739/150‑1 (MQ=255) aaCCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGt > 1:856763/1‑150 (MQ=255) aaCCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGt > 2:825513/1‑150 (MQ=255) gtcggtcAGGCCTTCGAGGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGAc > 2:838197/7‑150 (MQ=255) gtcggtcAGGCCTTCGAGGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGAc < 1:838197/144‑1 (MQ=255) tGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACgg < 1:825513/150‑1 (MQ=255) aGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGtt > 1:990890/1‑150 (MQ=255) cAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGtttt < 2:630529/150‑1 (MQ=255) aGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTg < 1:831414/150‑1 (MQ=255) cccTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTc < 2:856763/150‑1 (MQ=255) ccTCGAAGGCCTGACCGACAACATGACNGTACTGGCCGGTNGTTTCGNCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTCt > 1:1329178/1‑150 (MQ=255) ccTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTCt < 1:68478/150‑1 (MQ=255) tCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTgcggc < 1:1523437/131‑1 (MQ=255) tCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTgcggc > 2:1523437/1‑131 (MQ=255) | ATGACGAAAACAAAATTAACAAATCAGTCGCTTGCGGTGAAGATGTCTGCACACGCTCACCGACAGCCTCGTCGGTTAAACAACTACAAAAGGCGGATCAGCATGGCCGGACTGGACAAGCGCGTTGCAACCTATGAGCAGGCCCTCGAAGGCCTGACCGACAACATGACGGTACTGGCCGGTGGTTTCGGCCTGTGCGGCATCCCGGAAAACCTTATCAGCGAAATCAAGCGGCGCGGCGTCAAGGGCCTGACCGTGGTTTCCAACAACTGCGGCGTGGACGGTTTTGGTCT > NC_002947/3533264‑3533556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |