Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,551,118 A→G 8.7% Q76R (CAG→CGG) ‡ PP_3135 → glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,551,1180AG 8.7% 132.6 / 3.1 46Q76R (CAG→CGG) ‡PP_3135glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (24/18);  new base G (2/2);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

AGCGGCTGGCCAGTTACCTGGTGGTCGACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGC  >  NC_002947/3550986‑3551266
                                                                                                                                    |                                                                                                                                                     
agcgGCTGGCCAGTTACCTGGTGGTCGACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCa                                                                                                                                      >  2:1608424/1‑150 (MQ=255)
          cAGTTACCTGGTGGTCGACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTa                                                                                                                            >  2:1367499/1‑150 (MQ=255)
                ccTGGTGGTCGACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCtt                                                                                                                      <  2:1245323/150‑1 (MQ=255)
                 cTGGTGGTCGACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCa                                                                                                                                                       >  2:536859/1‑116 (MQ=255)
                 cTGGTGGTCGACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCa                                                                                                                                                       <  1:536859/116‑1 (MQ=255)
                          gACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAAt                                                                                                            >  1:1339309/1‑150 (MQ=255)
                           aCAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCAAGTGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGTGCAAGTATGCCTTGCTGCTCAATa                                                                                                           >  1:860699/1‑150 (MQ=255)
                                  cTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTgg                                                                                                                                               >  1:709839/1‑107 (MQ=255)
                                  cTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTgg                                                                                                                                               <  2:709839/107‑1 (MQ=255)
                                  cTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGc                                                                                                    >  2:27022/1‑150 (MQ=255)
                                      cgcgATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTc                                                                                                <  2:1614040/150‑1 (MQ=255)
                                         gATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGtt                                                                                             <  1:805568/150‑1 (MQ=255)
                                               tCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTgccgcc                                                                                       >  1:345401/1‑150 (MQ=255)
                                               tCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTgccgcc                                                                                       >  2:1317602/1‑150 (MQ=255)
                                                     cAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCg                                                                                 >  1:1592368/1‑150 (MQ=255)
                                                           aTGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑aa                                                                          >  2:234184/1‑150 (MQ=255)
                                                             ggCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACTGGTTGTTgg                                                                                                                                               <  2:1201721/80‑1 (MQ=255)
                                                             ggCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACTGGTTGTTgg                                                                                                                                               >  1:1201721/1‑80 (MQ=255)
                                                               cccAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑aaaaaa                                                                      >  2:841015/1‑150 (MQ=255)
                                                                      gtgCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAgg                                                                                                                                   >  1:1112014/1‑83 (MQ=255)
                                                                      gtgCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAgg                                                                                                                                   <  2:1112014/83‑1 (MQ=255)
                                                                         ccccGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTa                                                            >  2:954857/1‑150 (MQ=255)
                                                                          cccGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACACCCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCACCGACTCGCTGC‑AAAAAACGCTCGACTAc                                                           >  1:1601914/1‑150 (MQ=255)
                                                                             gAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATg                                                        <  2:1131364/150‑1 (MQ=255)
                                                                                  aCTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGa                                                      <  1:1224599/147‑1 (MQ=255)
                                                                                  aCTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGa                                                      >  2:1224599/1‑147 (MQ=255)
                                                                                  aCTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGc                                                   <  2:1339309/150‑1 (MQ=255)
                                                                                     gctgctgAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCGGCCTCGCTGC‑AACCAGCGCTCGACTACATGGActcgca                                                >  2:93474/1‑145 (MQ=255)
                                                                                            aGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGAt                                                                                                      >  2:54384/1‑90 (MQ=255)
                                                                                            aGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGAt                                                                                                      <  1:54384/90‑1 (MQ=255)
                                                                                              aaacaaGCAGAACGTGGGTTTAGGCCGAGCCAACAACCAGTTATTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGCTGCCTTCGTTGCCGCCGACTCGCTGA‑AAAAAACGATCAACTACATGGACGCACACCAGGAGTg                                       >  2:337758/2‑150 (MQ=255)
                                                                                                acaaGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTgcg                                     <  1:841015/150‑1 (MQ=255)
                                                                                                   aGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTgcggcg                                  >  1:495918/1‑150 (MQ=255)
                                                                                                    gCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCTCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGt                                 >  1:380544/1‑150 (MQ=255)
                                                                                                         aCGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTgg                            <  1:27022/150‑1 (MQ=255)
                                                                                                            tGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGCAAAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTgggg                          <  1:234184/150‑1 (MQ=255)
                                                                                                                tttCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCt                                                                               <  2:733584/93‑1 (MQ=255)
                                                                                                                tttCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCt                                                                               >  1:733584/1‑93 (MQ=255)
                                                                                                                      ccGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATCCCGATGACTTCGTTGCCATGGGTGGGCACC‑ATCAAATCCACTTCTACAGGGACGCCCACCCGGAGTGCGGCGTCGTGGTGGTGccccgcca               >  1:1427310/1‑146 (MQ=255)
                                                                                                                      ccGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGa               <  2:495918/150‑1 (MQ=255)
                                                                                                                        gggCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTAAGTTGAGAATCCTTCCTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAgg             <  1:604035/150‑1 (MQ=255)
                                                                                                                        gcgCCAACAACTGGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACa                                                          >  2:1499642/3‑105 (MQ=255)
                                                                                                                        gcgCCAACAACTGGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACa                                                          <  1:1499642/103‑1 (MQ=255)
                                                                                                                             aacaacCAGTTGTTGGCGCAAATCCAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTcgc        <  2:380544/150‑1 (MQ=255)
                                                                                                                                 acCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATg    <  1:93474/150‑1 (MQ=255)
                                                                                                                                  ccAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGt                        >  2:1604924/1‑129 (MQ=255)
                                                                                                                                  ccAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGt                        <  1:1604924/129‑1 (MQ=255)
                                                                                                                                   cAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGc  >  2:1528778/1‑150 (MQ=255)
                                                                                                                                    |                                                                                                                                                     
AGCGGCTGGCCAGTTACCTGGTGGTCGACAATGCCTCGCGCGATGACTCGGTGCAGCGCATGGCCCAGGTGTGCCCCGAGGCACTGCTGCTGAGCAACAAGCAGAACGTGGGTTTCGGCCGGGCCAACAACCAGTTGTTGGCGCACCTCAAGGGCAAGTATGCCTTGCTGCTCAATACCGATGCCTTCGTTGCCGCCGACTCGCTGC‑AAAAAACGCTCGACTACATGGACGCCCACCCGGAGTGCGGCGTACTGGGGGTGCGCCTGGAGGGTCGCGATGGC  >  NC_002947/3550986‑3551266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑