Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,743,607 C→A 6.8% A124A (GCC→GCA)  paoA → aromatic aldehyde dehydrogenase 2Fe‑2S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,743,6070CA 6.8% 185.9 / 2.6 59A124A (GCC→GCA) paoAaromatic aldehyde dehydrogenase 2Fe‑2S subunit
Reads supporting (aligned to +/- strand):  ref base C (24/31);  new base A (2/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

TCATGTGCGACGGGGCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGTTGATGACGGACCTGGCCCCGCCACAACGAGGGCCGAA  >  NC_002947/3743459‑3743756
                                                                                                                                                    |                                                                                                                                                     
tcatGTGCGACGGGGCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGccc                                                                                                                                                      <  1:671517/150‑1 (MQ=255)
     tgCGACGGGGCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGaa                                                                                                                                                 <  2:1259475/150‑1 (MQ=255)
     tgCGACGGGGCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGaa                                                                                                                                                 <  2:1259682/150‑1 (MQ=255)
           ggggCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGgcgc                                                                                                                                           <  1:47351/150‑1 (MQ=255)
             ggCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCCTTAATCAACCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGgcgcgc                                                                                                                                         <  1:1018334/150‑1 (MQ=255)
             ggCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGgcgcgc                                                                                                                                         >  1:270481/1‑150 (MQ=255)
              gCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGcc                                                                                                                                        >  2:1291243/1‑150 (MQ=255)
                cGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTa                                                                                                                                      <  1:1377009/150‑1 (MQ=255)
                     tGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGGTCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACAccc                                                                                                                                 <  1:1221730/150‑1 (MQ=255)
                         gACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGa                                                                                                                             <  1:1138418/150‑1 (MQ=255)
                           ccATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAgg                                                                                                                           <  2:956675/150‑1 (MQ=255)
                             aTCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGaatgg                                                                                                                         <  1:390735/150‑5 (MQ=255)
                                      cTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGaa                                                                                                                <  2:270481/150‑1 (MQ=255)
                                        ggCCAGCGGCGACGTTCTGCACCTCATGCAGCCTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGACATTCGCGAACa                                                                                                              <  2:620954/150‑1 (MQ=255)
                                               ggcgACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGc                                                                                                       >  2:860075/1‑150 (MQ=255)
                                                  gACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGc                                                                                                    >  2:884150/1‑150 (MQ=255)
                                                     gTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAAc                                                                                                 >  1:964224/1‑150 (MQ=255)
                                                       tCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCTTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCt                                                                                               <  1:785957/150‑1 (MQ=255)
                                                         tGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCt                                                                                               >  2:786408/1‑148 (MQ=255)
                                                         tGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCt                                                                                               <  1:786408/148‑1 (MQ=255)
                                                         tGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGACATTCGTGAACAGATGAGCGGCAACCtta                                                                                             >  2:785957/1‑149 (MQ=255)
                                                           cACCCCATGCAGCGTGCATTCGTCCCCCACGATTCTTTCCAGTGCGGTTAATGCTCTTCCAACTATTTCTGTTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGc                                                                                           <  1:849189/150‑1 (MQ=255)
                                                           cACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGc                                                                                           <  1:1196529/150‑1 (MQ=255)
                                                            aCCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCa                                                                                          >  2:1047774/1‑150 (MQ=255)
                                                             ccccATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAg                                                                                         <  1:860075/150‑1 (MQ=255)
                                                              cccATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCa                                                                                          <  1:398137/148‑1 (MQ=255)
                                                              cccATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCa                                                                                          >  2:398137/1‑148 (MQ=255)
                                                              cccATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAgg                                                                                        >  1:1488280/1‑150 (MQ=255)
                                                                    cAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTgtggt                                                                                  <  2:1488280/150‑1 (MQ=255)
                                                                         tGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCg                                                                                                      <  2:607971/125‑1 (MQ=255)
                                                                         tGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCg                                                                                                      >  1:607971/1‑125 (MQ=255)
                                                                           cATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCAtata                                                                           <  1:696011/150‑1 (MQ=255)
                                                                              tcatcaCCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCa                                                                        >  2:581966/1‑150 (MQ=255)
                                                                                 tcaCCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACa                                                                     >  1:1007779/1‑150 (MQ=255)
                                                                                     ccACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCACGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTgtggt                                                                                  <  2:949444/133‑1 (MQ=255)
                                                                                     ccACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCACGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTgtggt                                                                                  >  1:949503/1‑133 (MQ=255)
                                                                                     ccACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCACGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTgtggt                                                                                  >  1:949444/1‑133 (MQ=255)
                                                                                     ccACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCACGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTgtggt                                                                                  <  2:949503/133‑1 (MQ=255)
                                                                                       aCGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGa                                                                                                                             <  1:282712/88‑1 (MQ=255)
                                                                                       aCGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGa                                                                                                                             >  2:282712/1‑88 (MQ=255)
                                                                                       aCGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGc                                                                                                       <  1:1454467/110‑1 (MQ=255)
                                                                                       aCGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGc                                                                                                       >  2:1454467/1‑110 (MQ=255)
                                                                                              tttCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGTGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGt                                                        <  1:1349099/150‑1 (MQ=255)
                                                                                              tttCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGt                                                        >  2:1349099/1‑150 (MQ=255)
                                                                                                   aGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACAcc                                                                                                                                  <  1:613026/71‑1 (MQ=255)
                                                                                                   aGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACAcc                                                                                                                                  >  2:613026/1‑71 (MQ=255)
                                                                                                              tGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGc                                               >  2:1509117/1‑143 (MQ=255)
                                                                                                              tGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGc                                               <  1:1509117/143‑1 (MQ=255)
                                                                                                                cACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGtt                                      <  2:964224/150‑1 (MQ=255)
                                                                                                                          cAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGc                                                           >  2:252862/1‑119 (MQ=255)
                                                                                                                          cAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGc                                                           <  1:252862/119‑1 (MQ=255)
                                                                                                                            gATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGcc                                              >  2:353490/1‑130 (MQ=255)
                                                                                                                            gATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGcc                                              <  1:353490/130‑1 (MQ=255)
                                                                                                                            gATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGTTGATGACGGACCt                          <  1:581966/150‑1 (MQ=255)
                                                                                                                              tCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTgtggt                                                                                  >  2:703095/1‑92 (MQ=255)
                                                                                                                              tCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTgtggt                                                                                  <  1:703095/92‑1 (MQ=255)
                                                                                                                                tGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGTTGATGACGGACCTGGcc                      >  2:76848/1‑150 (MQ=255)
                                                                                                                                        aGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGTGAGGCCATCCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGGGCATATAGCAACATCGTCGGTGCGGTGGAAGAAGCGCTACCGTTGATGACGGACCTGGCCCCGCcaca              >  2:492703/1‑150 (MQ=255)
                                                                                                                                              gCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGtt                                      >  2:159240/1‑120 (MQ=255)
                                                                                                                                              gCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGtt                                      <  1:159240/120‑1 (MQ=255)
                                                                                                                                                   cccGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGTTGGTGACGGAACTGGCCCCGCCACAACGAGGGCCGa   >  1:258656/1‑150 (MQ=255)
                                                                                                                                                    ccGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGTTGATGACGGACCTGGCCCCGCCACAACGAGGGCCGaa  >  2:1028443/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCATGTGCGACGGGGCCGAACTGGTGACCATCGAAGGGCTGGCCAGCGGCGACGTTCTGCACCCCATGCAGCGTGCATTCATCACCCACGATGCTTTCCAGTGCGGTTACTGCACCCCGGGGCAGATCTGCTCCGCAGTCGGGCTTGCCCGCGAAGGGCGCGCCTACACCCGCGAGGCCATTCGCGAACAGATGAGCGGCAACCTTTGCAGGTGTGGTGCATATAGCAACATCGTCGCTGCGGTGGAAGAAGCCCTACCGTTGATGACGGACCTGGCCCCGCCACAACGAGGGCCGAA  >  NC_002947/3743459‑3743756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑