Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,788,441 G→C 6.6% Y79* (TAC→TAG)  PP_3350 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,788,4410GC 6.6% 170.4 / 2.0 55Y79* (TAC→TAG) PP_3350hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base C (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCCCGGC  >  NC_002947/3788297‑3788585
                                                                                                                                                |                                                                                                                                                
aCGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCg                                                                                                                                             >  1:416146/1‑150 (MQ=255)
    gTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTACCGGGcc                                                                                                                                         <  1:1473273/150‑1 (MQ=255)
        tctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                                                                                                     <  1:973841/150‑1 (MQ=255)
        tctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                                                                                                     <  1:841623/150‑1 (MQ=255)
        tctcGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAg                                                                                                                                     >  1:818103/1‑150 (MQ=255)
               cTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTAGTCCGAAGCATCGGCCCGCTAGCGGGACTCAGCCCCGAg                                                                                                                              >  2:979756/1‑150 (MQ=255)
                 ggACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                                                                                                            <  2:33347/150‑1 (MQ=255)
                   atgAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGc                                                                                                                          <  1:516927/148‑1 (MQ=255)
                       aGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCg                                                                                                                                             <  2:695713/127‑1 (MQ=255)
                       aGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCg                                                                                                                                             >  1:695713/1‑127 (MQ=255)
                             gCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCg                                                                                                                >  2:1542386/1‑150 (MQ=255)
                               aCGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGAc                                                                                                              >  2:495411/1‑150 (MQ=255)
                                  gCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGActct                                                                                                           <  1:495411/150‑1 (MQ=255)
                                       gtcgAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAg                                                                                                      >  1:1275014/1‑150 (MQ=255)
                                           aaCAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGa                                                                                                  <  1:535034/150‑1 (MQ=255)
                                           aaCAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGATCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGa                                                                                                  >  2:1636069/1‑150 (MQ=255)
                                             cAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATg                                                                                                <  2:989538/150‑1 (MQ=255)
                                               ggTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGt                                                                                              <  1:50020/150‑1 (MQ=255)
                                                  gCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGa                                                                                                                               >  1:386272/1‑114 (MQ=255)
                                                  gCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGa                                                                                                                               <  2:386272/114‑1 (MQ=255)
                                                     ggTCGGCATGGGCCGGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCa                                                                                        <  1:966402/150‑1 (MQ=255)
                                                       tCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAgg                                                                                      >  1:571819/1‑150 (MQ=255)
                                                           cATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCAt                                                                                  <  1:1542386/150‑1 (MQ=255)
                                                           cATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGCTac                                                                                                                                               <  2:1090452/89‑2 (MQ=255)
                                                            aTGGGCCTGCAGCCGTTGCTGCAGATAGGCACCGTCGTTCAGACCGCGCAGGACAAAGAAGGCTTTGTCCGAAGCATCGGGCCGGTAGTGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGACATc                                                                                 <  2:1604474/150‑1 (MQ=255)
                                                            aTGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGAGGAGCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATc                                                                                 >  1:935574/1‑150 (MQ=255)
                                                               tatcTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGAc                                                                                                              >  2:442089/4‑118 (MQ=255)
                                                               tatcTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGAc                                                                                                              <  1:442089/115‑1 (MQ=255)
                                                                gCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATc                                                                                 <  1:1120911/146‑1 (MQ=255)
                                                                gCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATc                                                                                 >  2:1120911/1‑146 (MQ=255)
                                                                            tgctgaAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGcc                                                                 <  1:98356/150‑1 (MQ=255)
                                                                              ctgcAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCtt                                                               >  1:1489125/1‑150 (MQ=255)
                                                                               tgcAGTTAGGAATCGTCGTTCAGCCCGCGCAGGCAAAAGAGGGGTTTGTCCGAAGCATCGGCCCGGTAGCGTGCCTCAGCGCCGGGTTGCAGCCAGGCCGGCTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTg                                                              <  1:979756/150‑1 (MQ=255)
                                                                                    aTAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                                                                                                            <  1:716213/83‑1 (MQ=255)
                                                                                    aTAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGtt                                                                                                                            >  2:716213/1‑83 (MQ=255)
                                                                                    aTAAGAATCCTCGTTCACCCTACACAGGCCAATGAAGAGTTTGTCCGAAGCGGCGCCCAAGTAGCGGGCCTCACCCCCGAGTTGCAGCGAATCCGGCTGCGAGAACCGATGGTAGCGCAGGCCCTGGAAAGGGGCAGTGGCCTTGGCagg                                                         <  2:621207/150‑1 (MQ=255)
                                                                                       ggAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGgtc                                                      <  2:818103/150‑1 (MQ=255)
                                                                                       ggAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGgtc                                                      >  2:22368/1‑150 (MQ=255)
                                                                                         aaTCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGgtcgt                                                    <  2:416146/150‑1 (MQ=255)
                                                                                             gtcgtTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCgag                                                <  1:22368/150‑1 (MQ=255)
                                                                                              tcgtTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGc                                                                                                                  >  2:998815/1‑83 (MQ=255)
                                                                                              tcgtTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGc                                                                                                                  <  1:998815/83‑1 (MQ=255)
                                                                                                     gCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGt                                                                                              <  1:854385/96‑1 (MQ=255)
                                                                                                     gCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGt                                                                                              >  2:854385/1‑96 (MQ=255)
                                                                                                      cccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTa                                       >  1:72201/1‑150 (MQ=255)
                                                                                                      cccGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTa                                       >  1:560394/1‑150 (MQ=255)
                                                                                                               gCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGAt                              >  2:371135/1‑150 (MQ=255)
                                                                                                                 cAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGCGCGGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATcc                            >  1:1171424/1‑150 (MQ=255)
                                                                                                                  aaaGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCa                           <  2:1489125/150‑1 (MQ=255)
                                                                                                                       agGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAg                      <  1:371135/150‑1 (MQ=255)
                                                                                                                          gTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAgggg                   >  1:1601238/1‑150 (MQ=255)
                                                                                                                           tttGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGc                                                                                                                  >  2:1207387/1‑54 (MQ=255)
                                                                                                                           tttGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGc                                                                                                                  <  1:1207387/54‑1 (MQ=255)
                                                                                                                                       aTCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTccc      >  2:882655/1‑150 (MQ=255)
                                                                                                                                           gCCCGCTACCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGAGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCccggc  >  2:254620/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACGGGTATTCTCGACCTGGACGAAGGTACGCACGGCATCGTCGAACAGGTGCAGGTCGGCATGGGCCTGCAGCCGTTGCTGCAGATAGGAATCGTCGTTCAGCCCGCGCAGGCCAAAGAAGGGTTTGTCCGAAGCATCGGCCCGGTAGCGGGCCTCAGCCCCGAGTTGCAGCCAGGCCGACTCTGACAGCCGATGGTAGCGCAGGCCATCGAAAGGGGCAGTGGCCTTGGCGGGGTCGTCGAGAAAGCGGTAGTCCTCGATCCAGCGAGGGGCCCCTGGCCTCCCCGGC  >  NC_002947/3788297‑3788585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑