| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,880,422 | C→G | 7.3% | A738G (GCC→GGC) | mexF → | multidrug RND transporter MexF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,880,422 | 0 | C | G | 7.3% | 173.3 / 4.6 | 55 | A738G (GCC→GGC) | mexF | multidrug RND transporter MexF |
| Reads supporting (aligned to +/- strand): ref base C (30/21); new base G (2/2); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GTTCCGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAG > NC_002947/3880273‑3880555 | gTTCCGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCAAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGGGCCACAAGTCGATgccgc > 1:789659/1‑150 (MQ=255) gCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCg > 2:1491070/1‑150 (MQ=255) cAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCgg < 2:1570036/150‑1 (MQ=255) aaTCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGa > 2:876874/1‑150 (MQ=255) gAATACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAg < 1:569004/150‑1 (MQ=255) gTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGa > 2:1044027/1‑150 (MQ=255) gTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGa > 1:1494779/1‑150 (MQ=255) tGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGGCGGCATCGActtgt > 1:1410567/1‑133 (MQ=255) tGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGGCGGCATCGActtgt < 2:1410567/137‑5 (MQ=255) aaCCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCAc > 1:1360483/1‑150 (MQ=255) gggTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGGGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGgtgt < 2:104798/150‑1 (MQ=255) cttACTAAGCGCCCTACACGCAAACCACGACCATCACCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAACTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTgg < 2:789659/149‑1 (MQ=255) tACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCg > 1:1620033/1‑150 (MQ=255) cGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGAt > 1:1157806/1‑150 (MQ=255) gAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATc > 2:487461/1‑150 (MQ=255) gcTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGa < 1:73751/150‑1 (MQ=255) aaCCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCa < 1:1485757/120‑1 (MQ=255) aaCCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCa > 2:1485757/1‑120 (MQ=255) ccAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCt > 2:160329/1‑150 (MQ=255) aGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGAAATCTTCGACACCCTGc < 1:56047/150‑1 (MQ=255) gCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTg < 1:1491070/150‑1 (MQ=255) gagCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTc > 1:1141238/1‑150 (MQ=255) gCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGc < 1:1171233/150‑1 (MQ=255) cacaACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGcc > 2:848860/1‑87 (MQ=255) cacaACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCGAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGCGCTCGCTg > 2:1376314/1‑150 (MQ=255) cacaACGCCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGcc < 1:848860/87‑1 (MQ=255) ccAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCg < 2:696322/68‑1 (MQ=255) ccAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCg > 1:696322/1‑68 (MQ=255) ccAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAAc > 1:505561/1‑150 (MQ=255) cAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACg > 1:1349249/1‑150 (MQ=255) gagCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGAc < 2:1349249/150‑1 (MQ=255) tGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGc > 1:849215/1‑70 (MQ=255) tGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGc < 2:849215/70‑1 (MQ=255) ggCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCaa < 1:487461/150‑1 (MQ=255) gCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGAc > 1:1602539/1‑96 (MQ=255) gCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGAc < 2:1602539/96‑1 (MQ=255) gCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAAc > 2:1624024/1‑150 (MQ=255) cTGTTCACCAGCTACCAGGTCAACGTGCCACGAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCttt > 1:776255/1‑150 (MQ=255) tGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTg > 1:202057/1‑150 (MQ=255) gTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTgg < 2:1360483/150‑1 (MQ=255) cACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCg > 1:1089073/1‑150 (MQ=255) aCCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACAGCTTTggagct > 1:14821/1‑146 (MQ=255) tACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTAc > 1:126210/1‑150 (MQ=255) ccAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCa > 2:727527/1‑150 (MQ=255) cAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCATGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTAc < 1:1124924/99‑1 (MQ=255) cAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCATGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTAc > 2:1124924/1‑99 (MQ=255) aGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAgg > 1:413398/1‑150 (MQ=255) tCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCttt > 2:1576773/1‑131 (MQ=255) tCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCttt < 1:1576773/131‑1 (MQ=255) tGCCACAAGTCGATGCCGCCATCAACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTcc < 1:160329/150‑1 (MQ=255) ccACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAg > 1:597801/1‑150 (MQ=255) ccACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAg < 1:1624024/150‑1 (MQ=255) cggTCGATGGCGGCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTggg < 2:1331304/86‑1 (MQ=255) cggTCGATGGCGGCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTggg > 1:1331304/3‑88 (MQ=255) gTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTc < 1:729393/108‑1 (MQ=255) gTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTc > 2:729393/1‑108 (MQ=255) cgccATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAAc < 1:981420/94‑1 (MQ=255) cgccATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAAc > 2:981420/1‑94 (MQ=255) | GTTCCGTCTGCAAATCGAAGACCGTGGCAACCTGGGTTACGAAGCGCTGTACAAGGAAACCCAGAACATCATCGCCAAGAGCCACAACGTCCCAGAGCTGGCAGGCCTGTTCACCAGCTACCAGGTCAACGTGCCACAAGTCGATGCCGCCATCGACCGGGAAAAGGCCAAGACCCACGGTGTGGCGATCACCGACATCTTCGACACCCTGCAGGTTTACCTGGGCTCGCTGTACACCAACGACTTCAACCGCTTTGGCCGCACTTACCAGGTCAACGTCCAG > NC_002947/3880273‑3880555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |