Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	343,610	T→A	11.0%	intergenic (‑163/‑77)	PP_5439 ← / → PP_5440	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	343,610	0	T	A	11.0%	129.3 / 7.9	46	intergenic (‑163/‑77)	PP_5439/PP_5440	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (19/21);  new base A (3/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

GTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGG  >  NC_002947/343462‑343754                                                                                                                                                     |                                                                                                                                                 gTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATg                                                                                                                                                 <  2:1374776/150‑1 (MQ=255)   gAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGtt                                                                                                                                               <  2:1359245/150‑1 (MQ=255)            aGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCAg                                                                                                                                      <  2:1637868/150‑1 (MQ=255)               gcgcGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCa                                                                                                                                       <  2:1239752/146‑1 (MQ=255)               gcgcGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCa                                                                                                                                       >  1:1239752/1‑146 (MQ=255)                   gTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCACCCATGTTGGCTGGCAGCCTGTCa                                                                                                                               >  1:168784/1‑150 (MQ=255)                           gcgcAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCa                                                                                                                       >  2:644109/1‑150 (MQ=255)                            cgcAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCaa                                                                                                                      >  1:9374/1‑150 (MQ=255)                               aaTTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTc                                                                                                                   <  2:9374/150‑1 (MQ=255)                                     ccGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTc                                                                                                             >  2:1024835/1‑150 (MQ=255)                                          aaaCCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtga                                                                                                        <  1:1212481/150‑1 (MQ=255)                                              ccAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTcc                                                                                                            <  1:400329/142‑1 (MQ=255)                                              ccAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTcc                                                                                                            >  2:400329/1‑142 (MQ=255)                                              ccAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAGGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtgagtga                                                                                                    >  1:917811/1‑150 (MQ=255)                                                 aGCGGCGCGCCTGGGCGCTGCAGCTCGGCAGGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATgc                                                                                                 <  1:820713/150‑1 (MQ=255)                                                       gcgcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGt                                                                                           >  1:827814/1‑150 (MQ=255)                                                         gtcTGGGCGCCTCAGCTTGAGATGTCTGTGGGATACATACCAAGATTATTCGGCCCGTGGGCTCTGCGTAGCTTCTCTGCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCCCTGCGCCAATTCGGCGTCCGTGACTGCTGCGCACGttt                                                                                         <  2:748601/148‑1 (MQ=255)                                                         gcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGt                                                                                                                                                >  1:24559/1‑95 (MQ=255)                                                         gcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGt                                                                                                                                                <  2:24559/95‑1 (MQ=255)                                                             gggCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTgcgc                                                                                     <  1:474782/150‑1 (MQ=255)                                                                    gCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAag                                                                              >  1:1208194/1‑150 (MQ=255)                                                                     cAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAagg                                                                             <  2:1003912/150‑1 (MQ=255)                                                                       gCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAaggag                                                                           <  2:168784/150‑1 (MQ=255)                                                                         ttGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACaaa                                                                               <  1:1156084/144‑1 (MQ=255)                                                                         ttGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACaaa                                                                               >  2:1156084/1‑144 (MQ=255)                                                                                 tCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCAc                                                                                             <  2:484743/122‑1 (MQ=255)                                                                                 tCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCAc                                                                                             >  1:484743/1‑122 (MQ=255)                                                                                  cTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAg                                                                <  1:323247/150‑1 (MQ=255)                                                                                   tgtgGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGc                                                               <  2:1208194/150‑1 (MQ=255)                                                                                       ggATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACtt                                                           >  2:1159596/1‑150 (MQ=255)                                                                                                tCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTgc                                                                                       <  2:1634731/113‑1 (MQ=255)                                                                                                tCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTgc                                                                                       >  1:1634731/1‑113 (MQ=255)                                                                                                             gACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGc                                     >  1:40654/1‑150 (MQ=255)                                                                                                             gACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACAGc                                     >  1:468393/1‑150 (MQ=255)                                                                                                                cGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCt                                                                                                                                   <  1:782531/53‑1 (MQ=255)                                                                                                                cGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCt                                                                                                                                   >  2:782531/1‑53 (MQ=255)                                                                                                                  tGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTacac                                <  2:827814/150‑1 (MQ=255)                                                                                                                        cTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGa                          <  1:644109/150‑1 (MQ=255)                                                                                                                            gTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGc                      <  2:40654/150‑1 (MQ=255)                                                                                                                              aGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAg                    >  1:1328080/1‑150 (MQ=255)                                                                                                                                    tctaCTGCTGCCAGCCATGTGTTCTGGCAGCCTTGCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTa              <  2:1328080/150‑1 (MQ=255)                                                                                                                                      tcCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACt            >  1:578269/1‑150 (MQ=255)                                                                                                                                        aggctgcCAGCCAACATGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTAt          >  2:707413/3‑150 (MQ=255)                                                                                                                                            tgcCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCaa      >  1:996116/1‑150 (MQ=255)                                                                                                                                            tgcCAGCCAACATGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCaa      >  1:1404341/1‑150 (MQ=255)                                                                                                                                                agccaTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGCCGTCCGTGCTCGATGCGCACGCTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCgg  >  1:1266487/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGG  >  NC_002947/343462‑343754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑