Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,051,307 T→C 5.6% intergenic (+27/+16) oprB‑III → / ← PP_3571 carbohydrate‑selective porin/acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,051,3070TC 5.6% 209.3 / 2.1 71intergenic (+27/+16)oprB‑III/PP_3571carbohydrate‑selective porin/acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base C (2/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

CAGGCCACGCCATGGCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGCACACCACGGTGGGGATGC  >  NC_002947/4051158‑4051456
                                                                                                                                                     |                                                                                                                                                      
cAGGCCACGCCATGGCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCAt                                                                                                                                                        >  2:415420/1‑150 (MQ=255)
  ggCCACGCCATGGCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATcc                                                                                                                                                      >  1:1545422/1‑150 (MQ=255)
     cACGCCATGGCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATccgcc                                                                                                                                                   <  2:313623/150‑1 (MQ=255)
     cACGCCATGGCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATccgcc                                                                                                                                                   >  1:840411/1‑150 (MQ=255)
      aCGCCATGGCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATccgccg                                                                                                                                                  >  1:497211/1‑150 (MQ=255)
             ggCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAAc                                                                                                                                           >  1:1298597/1‑150 (MQ=255)
                       gcgcCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCt                                                                                                                                 <  2:497211/150‑1 (MQ=255)
                       gcgcCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCt                                                                                                                                 >  2:1242311/1‑150 (MQ=255)
                         gcCCGGACGTGCAATACATCATCGATCCAGGGGCGTTCTCGAGACACCACATCGACAACGCACTCGTTGTCGGCCTGCTTGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCaaacaggc                                                                                                                               <  1:1601020/150‑7 (MQ=255)
                             ggACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCACCCCTCAAGCCTGTTTGa                                                                                                                           <  2:840411/150‑1 (MQ=255)
                              gACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAg                                                                                                                          <  2:832828/150‑1 (MQ=255)
                                 gTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTa                                                                                                                       <  1:415420/150‑1 (MQ=255)
                                   gCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGt                                                                                                                     >  1:34075/1‑150 (MQ=255)
                                         catcatCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCACCCCTCAAGCCTGTTTGAGGTAGTacacca                                                                                                               >  2:283235/1‑150 (MQ=255)
                                             atcGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCt                                                                                                           >  1:1461655/1‑150 (MQ=255)
                                               cGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTca                                                                                                         <  2:1461655/150‑1 (MQ=255)
                                                  gCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCaa                                                                                                                                     <  2:1545366/119‑1 (MQ=255)
                                                  gCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCaa                                                                                                                                     >  1:1545366/1‑119 (MQ=255)
                                                  gCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAgg                                                                                                                         >  1:1508425/1‑131 (MQ=255)
                                                  gCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAgg                                                                                                                         <  2:1508425/131‑1 (MQ=255)
                                                     aGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAgcagca                                                                                                   >  1:1368306/1‑150 (MQ=255)
                                                      ggggCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCAt                                                                                                  <  2:34075/150‑1 (MQ=255)
                                                       gggCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATg                                                                                                 <  2:1368306/150‑1 (MQ=255)
                                                            ttCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATc                                                                                           <  1:495131/150‑1 (MQ=255)
                                                               tcGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATcaca                                                                                        >  1:1633692/1‑150 (MQ=255)
                                                                       aGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCa                                                                                >  1:605263/1‑150 (MQ=255)
                                                                           aTCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTg                                                                            >  1:479202/1‑150 (MQ=255)
                                                                            tCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATcac                                                                                         >  2:476068/1‑136 (MQ=255)
                                                                            tCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATcac                                                                                         <  1:476068/136‑1 (MQ=255)
                                                                              gACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAACCGCATG‑GCATCACAGCCGCGCAACTGCTc                                                                         <  1:387601/150‑1 (MQ=255)
                                                                                 aaCGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGa                                                                                                                           >  1:62209/1‑98 (MQ=255)
                                                                                 aaCGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGa                                                                                                                           <  2:62209/98‑1 (MQ=255)
                                                                                 aaCGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGac                                                                      >  2:592/1‑150 (MQ=255)
                                                                                 aaCGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGac                                                                      >  2:1318458/1‑150 (MQ=255)
                                                                                    gCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCACCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAg                                                                                                                      >  2:1642044/1‑100 (MQ=255)
                                                                                    gCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCACCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAg                                                                                                                      <  1:1642044/100‑1 (MQ=255)
                                                                                    gCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCACCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAg                                                                                                                      >  2:1642433/1‑100 (MQ=255)
                                                                                    gCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCACCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGCAg                                                                                                                      <  1:1642433/100‑1 (MQ=255)
                                                                                      aCTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCcgcg                                                                                  <  1:1423144/133‑1 (MQ=255)
                                                                                      aCTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCcgcg                                                                                  >  2:1423144/1‑133 (MQ=255)
                                                                                      aCTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGa                                                                 >  2:348335/1‑150 (MQ=255)
                                                                                              tCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTTAAGCCGTTTTGAGGTAGTCCACCAGGCTCACCATCATT‑GCATCATAGCCGCGCAACTTCTCGACACTCACAAACTCa                                                         >  2:1162495/1‑150 (MQ=255)
                                                                                                ggCCTGCAGGTCAAGGCGCCCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAAc                                                            <  2:795706/145‑1 (MQ=255)
                                                                                                ggCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAAc                                                            >  1:795706/1‑145 (MQ=255)
                                                                                                  ccTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCg                                                     <  2:1633692/150‑1 (MQ=255)
                                                                                                   cTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCgg                                                    >  1:1557387/1‑150 (MQ=255)
                                                                                                    tGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGc                                                   >  1:841190/1‑150 (MQ=255)
                                                                                                          tCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTgg                                             <  2:841190/150‑1 (MQ=255)
                                                                                                          tCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTgg                                             <  2:479202/150‑1 (MQ=255)
                                                                                                            aaGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGt                                                                                                                               >  2:847274/1‑67 (MQ=255)
                                                                                                            aaGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGt                                                                                                                               <  1:847274/67‑1 (MQ=255)
                                                                                                            aaGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGcc                                           >  2:1465213/1‑150 (MQ=255)
                                                                                                              ggCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCt                                         >  1:622772/1‑150 (MQ=255)
                                                                                                               gCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTg                                        <  1:1242311/150‑1 (MQ=255)
                                                                                                                 gACCTTCTGAAACGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGt                                      <  2:664533/150‑1 (MQ=255)
                                                                                                                            ccgccgGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCa                           >  2:1001648/1‑150 (MQ=255)
                                                                                                                             cgccgGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAg                          >  1:756322/1‑150 (MQ=255)
                                                                                                                              gccgGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCa                                   <  1:1033574/140‑1 (MQ=255)
                                                                                                                              gccgGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCa                                   >  2:1033574/1‑140 (MQ=255)
                                                                                                                               ccgGGGCCGCACTGCGGCCCCATCCGCCTGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAggg                        <  1:592/150‑1 (MQ=255)
                                                                                                                                tggggCCGCAGTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAAc                                                            <  2:1591845/112‑1 (MQ=255)
                                                                                                                                tggggCCGCAGTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAAc                                                            >  1:1591845/2‑113 (MQ=255)
                                                                                                                                cgGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCtt                                                 >  2:614054/1‑124 (MQ=255)
                                                                                                                                cgGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCtt                                                 <  1:614054/124‑1 (MQ=255)
                                                                                                                                   ggCCGCCCTGCGGCCCCCTACGCCGGCGGACCCCTCAGGCCTGTTTGAGGTAGTCCACCAGCCTAAGC‑GCTTGTGCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGc                    <  1:283235/150‑1 (MQ=255)
                                                                                                                                          cTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATg                                                                                                 >  1:867694/1‑67 (MQ=255)
                                                                                                                                          cTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATg                                                                                                 <  2:867694/67‑1 (MQ=255)
                                                                                                                                              ggCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCATCAGCTTCAGCAGCCTG‑CCATCACAGCCATGCACCCGTTCTCCACTACCCTACTCCTCCGGCTTGCGGCCCTGGTCCATGCGGCCAGGGCCGCAATCCACGGTg         <  2:244442/150‑1 (MQ=255)
                                                                                                                                              ggCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGCACACCACGGTg         <  2:1545422/150‑1 (MQ=255)
                                                                                                                                              ggCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGCACACCACGGTg         >  1:1405748/1‑150 (MQ=255)
                                                                                                                                               gCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGCACACCACGGTgg        <  2:756322/150‑1 (MQ=255)
                                                                                                                                               gCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGCACACCACGGTgg        <  1:1001648/150‑1 (MQ=255)
                                                                                                                                                     tCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGCACACCACGGTGGGGATGc  <  2:605263/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAGGCCACGCCATGGCTGAACCTGCGCCCGGACGTGCAATACATCATCGAGCCAGGGGCGTTCTCGGGCAAAGACATCGACAACGCACTCGTGGTCGGCCTGCAGGTCAAGGCGACCTTCTGAACCGCCGGGGCCGCACTGCGGCCCCATCCGCCGGCGCAACCCTCAAGCCTGTTTGAGGTAGTCCACCAGCCTCAGCAGCATG‑GCATCACAGCCGCGCAACTGCTCGACACTGACAAACTCATCCGGCTTGTGGCCCTGGTCCATGCTGCCAGGGCCGCACACCACGGTGGGGATGC  >  NC_002947/4051158‑4051456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑