Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,141,212	T→A	7.7%	L44Q (CTG→CAG)	PP_3645 →	L‑proline amide hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,141,212	0	T	A	7.7%	152.8 / 5.4	53	L44Q (CTG→CAG)	PP_3645	L‑proline amide hydrolase
Reads supporting (aligned to +/- strand):  ref base T (27/21);  new base A (2/2);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

CCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACAAGCCACCGTGATCG  >  NC_002947/4141063‑4141358                                                                                                                                                      |                                                                                                                                                    ccTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTtggcct                                                                                                                                                     <  1:1461431/150‑1 (MQ=255)  cTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTtggcctg                                                                                                                                                    <  1:1333269/150‑1 (MQ=255)      aaGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCa                                                                                                                                                <  2:487840/150‑1 (MQ=255)        ggAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGACACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTCGGGCGGTCAGTTCGTTGGCCTGGCCAAc                                                                                                                                              >  2:424759/1‑150 (MQ=255)                cTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAAAAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACcg                                                                                                                                      >  1:1015672/1‑150 (MQ=255)                 tCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTTGCCGGGCCAAAGACTACcgc                                                                                                                                     >  2:379072/1‑150 (MQ=255)                 tCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACcgc                                                                                                                                     >  1:324865/1‑150 (MQ=255)                 tCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACcgc                                                                                                                                     >  1:1159326/1‑150 (MQ=255)                 tCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACcgc                                                                                                                                     <  1:944260/150‑1 (MQ=255)                 tCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCATGCCAACGaact                                                                                                                                         >  2:1443270/1‑143 (MQ=255)                 tCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCATGCCAACGaact                                                                                                                                         <  1:1443270/146‑4 (MQ=255)                          cAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGcc                                                                                                                            <  1:231010/150‑1 (MQ=255)                                   gCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATg                                                                                                                   >  1:345977/1‑150 (MQ=255)                                              cccTGACGGCACCAGCTAGCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGAAGTAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACgg                                                                                                        <  1:101038/149‑1 (MQ=255)                                              acCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACgg                                                                                                        <  2:1023095/150‑1 (MQ=255)                                                cTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACgg                                                                                                        >  1:892848/1‑148 (MQ=255)                                                cTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACgg                                                                                                        <  2:892848/148‑1 (MQ=255)                                                         ccAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCcgcg                                                                                             >  1:187932/1‑150 (MQ=255)                                                          cAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAAc                                                                                                                                              <  2:812955/100‑1 (MQ=255)                                                          cAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAAc                                                                                                                                              >  1:812955/1‑100 (MQ=255)                                                             cTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGc                                                                                                                             <  1:1201491/114‑1 (MQ=255)                                                             cTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGc                                                                                                                             >  2:1201491/1‑114 (MQ=255)                                                              tATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAG‑TCGTTGGCCAGGCCAACGaact                                                                                                                                         <  1:276503/100‑4 (MQ=255)                                                              tATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAG‑TCGTTGGCCAGGCCAACGaact                                                                                                                                         >  2:276503/1‑97 (MQ=255)                                                               aTCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGACCCAGGCAATGTGGGGCGGGCAAAACGTTGTCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCgg                                                                                       >  1:609322/1‑150 (MQ=255)                                                                  tGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCAGGGCGTGGGCCTGAACAACGAAATGTGGGGCGGTCAGTTCGTTGGCCTGCCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGgccg                                                                                    >  1:1330174/1‑150 (MQ=255)                                                                      tGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAACGCCGCCTACCGGCCGCCGa                                                                                >  2:208161/1‑150 (MQ=255)                                                                       gTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGac                                                                               >  1:1222680/1‑150 (MQ=255)                                                                            ccAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAAc                                                                                                                                              <  1:110691/82‑1 (MQ=255)                                                                            ccAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAAc                                                                                                                                              >  2:110691/1‑82 (MQ=255)                                                                              aGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGc                                                                        >  2:1590703/1‑150 (MQ=255)                                                                                ggCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTg                                                                      >  2:309759/1‑150 (MQ=255)                                                                                    aaCCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCaaa                                                                                                   <  1:300173/117‑1 (MQ=255)                                                                                    aaCCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCaaa                                                                                                   >  2:300173/1‑117 (MQ=255)                                                                                      cccGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGc                                                                >  2:584364/1‑150 (MQ=255)                                                                                         gtggtgCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTAt                                                             >  1:1178776/1‑150 (MQ=255)                                                                                               cTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGAc                                                       <  2:1159326/150‑1 (MQ=255)                                                                                                tGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCAACCGTCGACACACCGCTGGAAGGCTATGCCGAcc                                                      >  2:543281/1‑150 (MQ=255)                                                                                                  aTCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCg                                                         >  1:1641344/1‑145 (MQ=255)                                                                                                  aTCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCg                                                         <  2:1641344/145‑1 (MQ=255)                                                                                                  aTCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAg                                                    >  2:311889/1‑150 (MQ=255)                                                                                                         gCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCg                                            <  1:309759/150‑1 (MQ=255)                                                                                                                 cTGAACAAGGAACTGTGGGGCGGTCAGTTCGTTGGCCTGGCATACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACCCCGCTGGAAGGCTATGCCGACCAG‑CCCGCCGAACTGCtt                                    <  2:1330174/150‑1 (MQ=255)                                                                                                                   gAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGa                                  <  1:584364/150‑1 (MQ=255)                                                                                                                      cAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGacca                               >  2:436082/1‑150 (MQ=255)                                                                                                                       aaGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGaccac                              <  1:706002/150‑1 (MQ=255)                                                                                                                               gtgGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAAt                      <  2:609322/150‑1 (MQ=255)                                                                                                                                tgGGGCTGTCAGTTCGTTGGCCTTGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAAtt                     <  1:337121/150‑1 (MQ=255)                                                                                                                                      ggTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACaa               <  1:543281/150‑1 (MQ=255)                                                                                                                                      ggTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACaa               <  1:356409/150‑1 (MQ=255)                                                                                                                                          aGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACAAGCCa           >  1:104432/1‑150 (MQ=255)                                                                                                                                           gTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACAAGCCAc          >  1:1223893/1‑150 (MQ=255)                                                                                                                                            ttCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGATATGCTCGGCCACGGTCAAAGCCGCGTACCgg                                                                                       <  2:1346018/73‑1 (MQ=255)                                                                                                                                            ttCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGATATGCTCGGCCACGGTCAAAGCCGCGTACCgg                                                                                       >  1:1346018/1‑73 (MQ=255)                                                                                                                                                ttGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGACCTGCTTGACCACCTGCAAATTGCACAAGCCACCGtca     >  2:372085/1‑148 (MQ=255)                                                                                                                                                  ggcctggccAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGCAAAACCGCTGGAAGGCTCTG‑CGACCANTGGNACCGAACTGCTTGGCNACCTGCAAATTGCACAAGCCACCGTGATc   <  1:1104286/150‑1 (MQ=255)                                                                                                                                                   gcctggccAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCTACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACAAGCCACCGTGATCg  >  1:933780/1‑150 (MQ=255)                                                                                                                                                   gcctggccAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACAAGCCACCGTGATCg  >  1:538485/1‑150 (MQ=255)                                                                                                                                                     ctggccAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑c                                                  >  2:505521/1‑100 (MQ=255)                                                                                                                                                     ctggccAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑c                                                  <  1:505521/100‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CCTTCAAGGAGCGCCCTCCATGATTCAGCCTGTCGCTGAACGCACACCTGCCGGCACCAGCTATCTGGATGTCGGCCAGGGCCAACCCGTGGTGCTGATCCACGGCGTGGGCCTGAACAAGGAAATGTGGGGCGGTCAGTTCGTTGGCCTGGCCAACGACTACCGCGTCATCGCCTACGACATGCTCGGCCACGGTCAAAGCCGCGTACCGGCCGCCGACACACCGCTGGAAGGCTATGCCGACCAG‑CTCGCCGAACTGCTTGACCACCTGCAAATTGCACAAGCCACCGTGATCG  >  NC_002947/4141063‑4141358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑