Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,142,724 C→T 6.2% I271I (ATC→ATT)  PP_3646 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,142,7240CT 6.2% 190.1 / 2.9 64I271I (ATC→ATT) PP_3646aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (27/33);  new base T (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

GCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCAACCCGCAG  >  NC_002947/4142580‑4142868
                                                                                                                                                |                                                                                                                                                
gcgcAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATg                                                                                                                                              <  1:390262/149‑1 (MQ=255)
gcgcAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATg                                                                                                                                              >  2:390262/1‑149 (MQ=255)
       aTCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTg                                                                                                                                      <  1:1479751/150‑1 (MQ=255)
            cTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACGGCGCCATTGATGgcgct                                                                                                                                         <  1:1073352/142‑2 (MQ=255)
            cTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACGGCGCCATTGATGgcgct                                                                                                                                         >  2:1073352/1‑141 (MQ=255)
             ttCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGc                                                                                                                                >  1:583299/1‑150 (MQ=255)
                  cggcggTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTa                                                                                                                           >  2:936938/1‑150 (MQ=255)
                             ccACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCg                                                                                                                <  2:617406/150‑1 (MQ=255)
                             ccACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCg                                                                                                                <  1:557267/150‑1 (MQ=255)
                              cACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCgg                                                                                                               >  1:1228344/1‑150 (MQ=255)
                               aCCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCggg                                                                                                              <  1:549316/150‑1 (MQ=255)
                                ccgcccgcCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGc                                                                                                             <  1:1575393/150‑1 (MQ=255)
                                  gcccgccACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCaa                                                                                                           >  2:1344035/1‑150 (MQ=255)
                                           gTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATCCCGCGTCCGGGCAAAGCTGCGTg                                                                                                  <  2:25123/150‑1 (MQ=255)
                                                 cGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCTGGCAAAGCTGc                                                                                                     <  2:293755/141‑1 (MQ=255)
                                                 cGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCTGGCAAAGCTGc                                                                                                     >  1:293755/1‑141 (MQ=255)
                                                       agcGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCgcgc                                                                                      <  1:50032/150‑1 (MQ=255)
                                                       agcGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCgcgc                                                                                      <  1:1311085/150‑1 (MQ=255)
                                                         cgcCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCct                                                                                    <  1:117099/150‑1 (MQ=255)
                                                          gcCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCctg                                                                                   >  1:326908/1‑150 (MQ=255)
                                                            cGAAAACTTCGCCAGGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAAt                                                                                                                                               >  2:282541/1‑88 (MQ=255)
                                                            cGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAAt                                                                                                                                               <  1:282541/88‑1 (MQ=255)
                                                             gAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCctgctg                                                                                <  1:210370/150‑1 (MQ=255)
                                                                   ttCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAg                                                                          <  2:1540697/150‑1 (MQ=255)
                                                                   ttCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGACGGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAg                                                                          >  2:1365857/1‑150 (MQ=255)
                                                                     cGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGa                                                                        <  2:1037111/150‑1 (MQ=255)
                                                                     cGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGa                                                                        <  1:1096243/150‑1 (MQ=255)
                                                                     cGCCAAGCTGTCGCGGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGa                                                                        <  2:782727/150‑1 (MQ=255)
                                                                        cAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGa                                                                     >  1:1554/1‑150 (MQ=255)
                                                                        cAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGa                                                                     >  2:1273336/1‑150 (MQ=255)
                                                                              gTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCtt                                                               <  1:406682/150‑1 (MQ=255)
                                                                              gTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCtt                                                               <  2:1554/150‑1 (MQ=255)
                                                                                      aaCTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGt                                                       <  1:1292488/150‑1 (MQ=255)
                                                                                         tGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGATGGTGCAGGATGAAATCTTCGACGAGTtcg                                                    >  1:821090/1‑150 (MQ=255)
                                                                                           ggcggcAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTtcgtc                                                  <  1:1273336/150‑1 (MQ=255)
                                                                                             cggcAAGTGGCCGAACATCATCTTCGCCGGCGCCGACCTGGAGAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGa                                                >  2:23830/1‑150 (MQ=255)
                                                                                                 aaGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCTTTTCCTGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAgcgc                                            <  1:192509/150‑1 (MQ=255)
                                                                                                 aaGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAgcgc                                            <  2:1228344/150‑1 (MQ=255)
                                                                                                      gCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGAt                                       <  1:1629298/150‑1 (MQ=255)
                                                                                                        cGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTAt                                                                                                                          >  1:713380/1‑65 (MQ=255)
                                                                                                        cGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTAt                                                                                                                          <  2:713380/65‑1 (MQ=255)
                                                                                                        cGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTg                                     >  1:1193275/1‑150 (MQ=255)
                                                                                                                atcTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGcc                             >  1:28277/1‑150 (MQ=255)
                                                                                                                   ttCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAg                          >  2:801464/1‑150 (MQ=255)
                                                                                                                    tCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAgc                         >  1:1631428/1‑150 (MQ=255)
                                                                                                                          acgccgacCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGcgcatcc                   <  2:821090/150‑1 (MQ=255)
                                                                                                                              ctacCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGcgcatccgcat               >  2:1471669/3‑150 (MQ=255)
                                                                                                                              cgacCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGcgcatccgcat               >  2:626934/1‑150 (MQ=255)
                                                                                                                               gacCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATATATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGcgcatccgcatc              <  2:1621071/150‑1 (MQ=255)
                                                                                                                                   tGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTgtt                                       >  1:1316891/1‑119 (MQ=255)
                                                                                                                                   tGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTgtt                                       <  2:1316891/121‑3 (MQ=255)
                                                                                                                                    ggACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCaa         >  1:1156733/1‑150 (MQ=255)
                                                                                                                                     gACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTcgacga                                                         >  1:131188/1‑101 (MQ=255)
                                                                                                                                     gACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTcgacga                                                         <  2:131188/101‑1 (MQ=255)
                                                                                                                                      acggcgcCATTGATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCt                                                                <  2:311783/90‑1 (MQ=255)
                                                                                                                                      acggcgcCATTGATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCt                                                                >  1:311783/4‑93 (MQ=255)
                                                                                                                                         gcgcCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCCCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCAACCCGc    >  1:1392777/1‑150 (MQ=255)
                                                                                                                                          cgcCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCAACCCGCa   <  2:304753/150‑1 (MQ=255)
                                                                                                                                          cgcCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCAACCCGCa   <  2:76427/150‑1 (MQ=255)
                                                                                                                                           gcCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCAACCCGCAg  >  2:1390065/1‑150 (MQ=255)
                                                                                                                                           gcCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGCATGGAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGTGCCCAGGCCATCAGCATCGGCAACCCGCAg  >  1:1594105/1‑150 (MQ=255)
                                                                                                                                           cccATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCAACCCGCAg  <  2:583299/149‑1 (MQ=255)
                                                                                                                                              aTCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTcgacgaac                                                       >  2:797120/1‑92 (MQ=255)
                                                                                                                                              aTCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTcgacgaac                                                       <  1:797120/94‑3 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCCGCGTCCGGGCAAAGCTGCGTGGCCGGCTCGCGCCTGCTGGTGCAGGATGAAATCTTCGACGAGTTCGTCGAGCGCCTGATTGCCCGCGCCAAGCGCATCCGCATCGGCAACCCGCAG  >  NC_002947/4142580‑4142868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑