Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,160,706 G→C 9.7% intergenic (+28/+93) PP_3662 → / ← PP_3663 hypothetical protein/GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,160,7060GC 9.7% 146.9 / 6.2 49intergenic (+28/+93)PP_3662/PP_3663hypothetical protein/GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (19/25);  new base C (3/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CCACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCC  >  NC_002947/4160558‑4160852
                                                                                                                                                    |                                                                                                                                                  
ccACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGa                                                                                                                                                   <  1:1040444/150‑1 (MQ=255)
    ggTGGCAGCGGTGTCCTGGAAACGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACATTCGCGGCATGTGGCAACCTGACCCGCTGCTTTACTGAGGACTGGCGTGGATGcc                                                                                                                                               >  2:458577/1‑150 (MQ=255)
        aCAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTTCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTc                                                                                                                                           <  2:596727/150‑1 (MQ=255)
        aCAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTc                                                                                                                                           >  1:984907/1‑150 (MQ=255)
           gcgcCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGaa                                                                                                                                        <  2:832745/150‑1 (MQ=255)
                gTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCgg                                                                                                                                   <  2:569180/150‑1 (MQ=255)
                gTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCgg                                                                                                                                   <  2:1100097/150‑1 (MQ=255)
                 tACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCggg                                                                                                                                  <  2:1347246/150‑1 (MQ=255)
                      gACCCGCTGGGCCGGCTGCGGCAGTCATTCGCCGACCAGCACCGGGTGAAGCTGCGCGGCGGAACGTCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTATACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGccc                                                                                                                             >  2:1282/1‑149 (MQ=255)
                       aCCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGAACAGAAAAGGCTGTCCCCGGCCGGCGGCTCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCGTTACTGAGGACGGGCATGGAGGCCCGTCGAACGCGGGGGCCgg                                                                                                                            >  1:850130/1‑150 (MQ=255)
                        cccGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCggg                                                                                                                           <  1:300455/150‑1 (MQ=255)
                                    gctgctGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGt                                                                                                               <  1:871166/150‑1 (MQ=255)
                                          gcAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTAc                                                                                                         <  1:274126/150‑1 (MQ=255)
                                          gcAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTAc                                                                                                         <  1:1003305/150‑1 (MQ=255)
                                                aTTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGt                                                                                                                                            >  1:1052509/1‑109 (MQ=255)
                                                aTTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATCCATGCCCGt                                                                                                                                            <  2:1052509/109‑1 (MQ=255)
                                                    gtcgACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCca                                                                                               <  2:850130/150‑1 (MQ=255)
                                                     tcgACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGccc                                                                                                                                              <  2:125936/102‑1 (MQ=255)
                                                     tcgACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGccc                                                                                                                                              >  1:125936/1‑102 (MQ=255)
                                                          cAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATggg                                                                                         >  2:795081/1‑150 (MQ=255)
                                                             cACCGGGTGAAGCTGCCCGGCGGCGCTGCGTATGTACCGTGCTACCAGGTGGCAAGCTGTCCCGCTGCTTTATTGAGGGCGGATAGCGACGCCAGTCGAACCCGGGCGCTGAGGGATGGGAGCGTCTGTACGGTTCCCCCACATCGGTTa                                                                                      <  2:123139/150‑1 (MQ=255)
                                                             cACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTa                                                                                      <  1:795081/150‑1 (MQ=255)
                                                               ccGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCTGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACa                                                                                    <  1:706336/150‑1 (MQ=255)
                                                                  gATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCgg                                                                                                                                   >  1:1312840/1‑100 (MQ=255)
                                                                  gATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCgg                                                                                                                                   <  2:1312840/100‑1 (MQ=255)
                                                                       aGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGCATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGca                                                                            >  1:1598587/1‑149 (MQ=255)
                                                                                               tACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGc                                                                                                                 >  2:401122/1‑89 (MQ=255)
                                                                                               tACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGc                                                                                                                 <  1:401122/89‑1 (MQ=255)
                                                                                               tACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCAc                                                    >  1:1346486/1‑150 (MQ=255)
                                                                                                 ccGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTg                                                  >  2:1614259/1‑150 (MQ=255)
                                                                                                    tGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCCCCTAGGTGACTCGCTCACTGCCg                                               <  1:958283/150‑1 (MQ=255)
                                                                                                       tATCAGGTGGCAACCTGACCCGCTGCTTTACTGCGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAg                                            >  2:954462/1‑150 (MQ=255)
                                                                                                           aGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAATCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCa                                        >  1:222511/1‑150 (MQ=255)
                                                                                                           aGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCAc                                                                     >  1:1438116/1‑121 (MQ=255)
                                                                                                           aGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCAc                                                                     <  2:1438116/121‑1 (MQ=255)
                                                                                                           aGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCa                                        >  1:660976/1‑150 (MQ=255)
                                                                                                             gTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAac                                      <  2:1346486/150‑1 (MQ=255)
                                                                                                                gCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGACCGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAaccac                                   >  1:1351374/1‑150 (MQ=255)
                                                                                                                  aaCCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCAccg                                 >  1:1504722/1‑150 (MQ=255)
                                                                                                                                ctTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGt                                                               >  1:517548/1‑106 (MQ=255)
                                                                                                                                ctTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGt                                                               <  2:517548/106‑1 (MQ=255)
                                                                                                                                ctTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAAc                   <  1:421126/150‑1 (MQ=255)
                                                                                                                                    aCTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGaa               <  1:1614259/150‑1 (MQ=255)
                                                                                                                                      tGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATg             <  1:458577/150‑1 (MQ=255)
                                                                                                                                          gACGGGCATCCATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCt                                                       >  1:734319/1‑104 (MQ=255)
                                                                                                                                          gACGGGCATCCATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCt                                                       <  2:734319/104‑1 (MQ=255)
                                                                                                                                               gCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGAATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGtt                                          <  2:1376718/112‑1 (MQ=255)
                                                                                                                                               gCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGAATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGtt                                          >  1:1376718/1‑112 (MQ=255)
                                                                                                                                                 aTGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGccc  >  1:1092769/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCACGGTGACAGCGCCGTACTGGACCCGCTGGGCCGGCTGCTGCAGGCATTCGTCGACCAGCACCGGATGAAGCTGCCCGGCGGCGCGGCGTATGTACCGTGCTATCAGGTGGCAACCTGACCCGCTGCTTTACTGAGGACGGGCATGGATGCCCGTCGAACGCGGGGGCCGGGTGACGGGAGCGTCTGTACGGATCACCCACATGGGTTACATGAAAGCTCCCCCACCTAGGTGACTCGCTCACTGCCGGAGTTCAACCACCGCCCGCTCGAAAAACCGAATGCTGTCGCGCCC  >  NC_002947/4160558‑4160852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑