Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,196,336 G→A 15.7% intergenic (+134/+235) PP_5618 → / ← PP_5619 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,196,3360GA15.7% 134.6 / 14.6 62intergenic (+134/+235)PP_5618/PP_5619Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (4/6);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTA  >  NC_002947/4196196‑4196482
                                                                                                                                            |                                                                                                                                                  
cTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACg                                                                                                                                           >  2:348040/1‑150 (MQ=255)
    tGACGGTAAGCCCCTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGgctc                                                                                                                                       <  2:693038/150‑1 (MQ=255)
      aCGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCgg                                                                                                                                     <  2:1013876/150‑1 (MQ=255)
      aCGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCgg                                                                                                                                     <  2:1021588/150‑1 (MQ=255)
           aaGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCggttcac                                                                                                                                <  1:348040/150‑6 (MQ=255)
              ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCgg                                                                                                                             >  2:727254/1‑150 (MQ=255)
              ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCggttca                                                                                                                                 <  1:1541594/146‑5 (MQ=255)
              ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCggttca                                                                                                                                 >  2:1541594/1‑142 (MQ=255)
              ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCCTCCGCGGTGTCGACGTCTCGGCCCTCCgg                                                                                                                             <  1:862917/150‑1 (MQ=255)
               cACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                            >  2:928392/1‑150 (MQ=255)
               cACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                            >  1:452145/1‑150 (MQ=255)
               cACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                            <  2:452145/150‑1 (MQ=255)
                         ttCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAg                                                                                                                  >  1:595486/1‑150 (MQ=255)
                         ttCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGATCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTTTGGGCGCCGATCTGGAGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAg                                                                                                                  >  1:603920/1‑150 (MQ=255)
                               tctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGCTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAAGCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGc                                                                                                            >  1:1566689/1‑150 (MQ=255)
                                 tACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGCCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGTGCAGCCATCAGTCACGCtt                                                                                                          >  1:320408/1‑150 (MQ=255)
                                   cgcgAGGAGGGAAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTgc                                                                                                        >  2:1223250/1‑150 (MQ=255)
                                           gggTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCa                                                                                                >  1:1555171/1‑150 (MQ=255)
                                              tAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCa                                                                                                                   <  2:1348024/128‑1 (MQ=255)
                                              tAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCa                                                                                                                   >  1:1348024/1‑128 (MQ=255)
                                               aaTGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTg                                                                                            >  1:399664/1‑150 (MQ=255)
                                                 tGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGcc                                                                                          <  1:928392/150‑1 (MQ=255)
                                                     ccGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgca                                                                                      <  2:595486/150‑1 (MQ=255)
                                                            gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCgg                                                                                                                                     >  2:1135701/1‑96 (MQ=255)
                                                            gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCgg                                                                                                                                     <  1:1135701/96‑1 (MQ=255)
                                                            gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGaaa                                                                               <  1:727254/150‑1 (MQ=255)
                                                            gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGaaa                                                                               >  2:599082/1‑150 (MQ=255)
                                                              tCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAAtt                                                                             >  1:1006223/1‑150 (MQ=255)
                                                               cGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGTGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTTCAGCAGAAATTc                                                                            <  2:1570767/150‑1 (MQ=255)
                                                                 cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTgcgc                                                                                                      <  2:165473/122‑1 (MQ=255)
                                                                 cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTgcgc                                                                                                      >  1:165473/1‑122 (MQ=255)
                                                                   tGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGaa                                                                        <  1:3616/150‑1 (MQ=255)
                                                                        tCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCgg                                                                                                                                     >  2:1646136/1‑84 (MQ=25)
                                                                        tCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCgg                                                                                                                                     <  1:1646136/84‑1 (MQ=25)
                                                                               gCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGATCCGTGGACGGTGTTGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGGCCCACTTGCCTGCAGCAGAAATTCTGAGCATGCTCTGTTc                                                            >  1:215362/1‑150 (MQ=39)
                                                                               gCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTc                                                            <  2:1206370/150‑1 (MQ=255)
                                                                               gCAGAGCTTGCTAAGGACTGCGGGCCCTATGGGCGCGGCTGTCGTGAAGCGAGCCGTCGACGGGGTGGACGTCTCGGCCCTCCGGGCAGCCATCAGTCGCGGTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTc                                                            <  2:317018/150‑1 (MQ=255)
                                                                                   aTCTTGATAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACAGTGTCGACGTCTCGGCCCTCCGAGCAGCCATCAGTCACGATTGCGCGCCCCACTTGCCTGCAGCACAAATTCTGAAAATGCTCTTTTCGAcc                                                        >  2:680780/1‑150 (MQ=38)
                                                                                     cTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTGGTGAACCGAGCAGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGc                                                      <  2:146612/150‑1 (MQ=255)
                                                                                      ttGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgca                                                                                      <  2:1581996/117‑1 (MQ=255)
                                                                                      ttGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgca                                                                                      >  1:1581996/1‑117 (MQ=255)
                                                                                         cTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                  >  1:136854/1‑150 (MQ=255)
                                                                                         cTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAACAGTTGATTATGTCGACGTCTCGGCCCTTCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                  >  1:692944/1‑150 (MQ=38)
                                                                                           aaGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGCCGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATgg                                                >  2:111381/1‑150 (MQ=255)
                                                                                               ccTGCGTGCCCTATGCGCCCGGCTGTCGTGAACCGAGCCGTCGACGTTGTCGACGGCTCGCCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATtg                                            <  2:603920/149‑1 (MQ=255)
                                                                                                         cTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                  <  2:725698/150‑1 (MQ=255)
                                                                                                         cTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                  <  2:136854/150‑1 (MQ=255)
                                                                                                          tATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                 <  2:235257/134‑1 (MQ=255)
                                                                                                          tATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                 >  1:235257/1‑134 (MQ=255)
                                                                                                                  cGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTc                         <  1:567406/150‑1 (MQ=255)
                                                                                                                   ggCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCa                        >  2:1495203/1‑150 (MQ=255)
                                                                                                                      tGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGaa                     <  2:1555171/150‑1 (MQ=255)
                                                                                                                        tCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                  <  1:208747/135‑1 (MQ=255)
                                                                                                                        tCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                  >  2:208747/1‑135 (MQ=255)
                                                                                                                         cGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                  <  1:1223250/150‑1 (MQ=255)
                                                                                                                          gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGc                                                      >  1:1381335/1‑113 (MQ=255)
                                                                                                                          gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGc                                                      <  2:1381335/113‑1 (MQ=255)
                                                                                                                          gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                  >  1:932271/1‑133 (MQ=255)
                                                                                                                          gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                  <  2:932271/133‑1 (MQ=255)
                                                                                                                                cGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCg           <  2:692944/150‑1 (MQ=255)
                                                                                                                                         gACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTa  <  2:399664/150‑1 (MQ=255)
                                                                                                                                         gACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTa  >  2:938392/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
CTGGTGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTA  >  NC_002947/4196196‑4196482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑