Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,196,346 T→G 11.9% intergenic (+144/+225) PP_5618 → / ← PP_5619 Cro/CI family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,196,3460TG11.9% 143.4 / 12.5 63intergenic (+144/+225)PP_5618/PP_5619Cro/CI family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/26);  new base G (2/6);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

TGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGCCCA  >  NC_002947/4196200‑4196492
                                                                                                                                                  |                                                                                                                                                  
tGACGGTAAGCCCCTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGgctc                                                                                                                                                 <  2:693038/150‑1 (MQ=255)
  aCGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCgg                                                                                                                                               <  2:1013876/150‑1 (MQ=255)
  aCGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCgg                                                                                                                                               <  2:1021588/150‑1 (MQ=255)
       aaGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCggttcac                                                                                                                                          <  1:348040/150‑6 (MQ=255)
          ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCgg                                                                                                                                       >  2:727254/1‑150 (MQ=255)
          ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCggttca                                                                                                                                           <  1:1541594/146‑5 (MQ=255)
          ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACACCGTCGACGGCTCggttca                                                                                                                                           >  2:1541594/1‑142 (MQ=255)
          ccACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCCTCCGCGGTGTCGACGTCTCGGCCCTCCgg                                                                                                                                       <  1:862917/150‑1 (MQ=255)
           cACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                                      >  1:452145/1‑150 (MQ=255)
           cACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                                      <  2:452145/150‑1 (MQ=255)
           cACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCggg                                                                                                                                      >  2:928392/1‑150 (MQ=255)
                     ttCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAg                                                                                                                            >  1:595486/1‑150 (MQ=255)
                     ttCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGATCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTTTGGGCGCCGATCTGGAGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAg                                                                                                                            >  1:603920/1‑150 (MQ=255)
                           tctACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGCTCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAAGCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGc                                                                                                                      >  1:1566689/1‑150 (MQ=255)
                             tACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGCCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGTGCAGCCATCAGTCACGCtt                                                                                                                    >  1:320408/1‑150 (MQ=255)
                               cgcgAGGAGGGAAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTgc                                                                                                                  >  2:1223250/1‑150 (MQ=255)
                                       gggTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCa                                                                                                          >  1:1555171/1‑150 (MQ=255)
                                          tAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCa                                                                                                                             <  2:1348024/128‑1 (MQ=255)
                                          tAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCa                                                                                                                             >  1:1348024/1‑128 (MQ=255)
                                           aaTGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTg                                                                                                      >  1:399664/1‑150 (MQ=255)
                                             tGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGcc                                                                                                    <  1:928392/150‑1 (MQ=255)
                                                 ccGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgca                                                                                                <  2:595486/150‑1 (MQ=255)
                                                        gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCgg                                                                                                                                               >  2:1135701/1‑96 (MQ=255)
                                                        gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCgg                                                                                                                                               <  1:1135701/96‑1 (MQ=255)
                                                        gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGaaa                                                                                         <  1:727254/150‑1 (MQ=255)
                                                        gCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGaaa                                                                                         >  2:599082/1‑150 (MQ=255)
                                                          tCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAAtt                                                                                       >  1:1006223/1‑150 (MQ=255)
                                                           cGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGTGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTTCAGCAGAAATTc                                                                                      <  2:1570767/150‑1 (MQ=255)
                                                             cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTgcgc                                                                                                                <  2:165473/122‑1 (MQ=255)
                                                             cATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTgcgc                                                                                                                >  1:165473/1‑122 (MQ=255)
                                                               tGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGaa                                                                                  <  1:3616/150‑1 (MQ=255)
                                                                    tCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCgg                                                                                                                                               >  2:1646136/1‑84 (MQ=25)
                                                                    tCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGACGTCGACACCGTCGACGGCTCgg                                                                                                                                               <  1:1646136/84‑1 (MQ=25)
                                                                           gCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGATCCGTGGACGGTGTTGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGGCCCACTTGCCTGCAGCAGAAATTCTGAGCATGCTCTGTTc                                                                      >  1:215362/1‑150 (MQ=39)
                                                                           gCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTc                                                                      <  2:1206370/150‑1 (MQ=255)
                                                                           gCAGAGCTTGCTAAGGACTGCGGGCCCTATGGGCGCGGCTGTCGTGAAGCGAGCCGTCGACGGGGTGGACGTCTCGGCCCTCCGGGCAGCCATCAGTCGCGGTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTc                                                                      <  2:317018/150‑1 (MQ=255)
                                                                               aTCTTGATAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACAGTGTCGACGTCTCGGCCCTCCGAGCAGCCATCAGTCACGATTGCGCGCCCCACTTGCCTGCAGCACAAATTCTGAAAATGCTCTTTTCGAcc                                                                  >  2:680780/1‑150 (MQ=38)
                                                                                 cTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTGGTGAACCGAGCAGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGc                                                                <  2:146612/150‑1 (MQ=255)
                                                                                  ttGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgca                                                                                                >  1:1581996/1‑117 (MQ=255)
                                                                                  ttGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTgca                                                                                                <  2:1581996/117‑1 (MQ=255)
                                                                                     cTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                            >  1:136854/1‑150 (MQ=255)
                                                                                     cTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAACAGTTGATTATGTCGACGTCTCGGCCCTTCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCAt                                                            >  1:692944/1‑150 (MQ=38)
                                                                                       aaGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGCCGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATgg                                                          >  2:111381/1‑150 (MQ=255)
                                                                                           ccTGCGTGCCCTATGCGCCCGGCTGTCGTGAACCGAGCCGTCGACGTTGTCGACGGCTCGCCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATtg                                                      <  2:603920/149‑1 (MQ=255)
                                                                                                     cTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                            <  2:725698/150‑1 (MQ=255)
                                                                                                     cTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                            <  2:136854/150‑1 (MQ=255)
                                                                                                      tATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                           <  2:235257/134‑1 (MQ=255)
                                                                                                      tATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATg                                                           >  1:235257/1‑134 (MQ=255)
                                                                                                              cGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTc                                   <  1:567406/150‑1 (MQ=255)
                                                                                                               ggCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCa                                  >  2:1495203/1‑150 (MQ=255)
                                                                                                                  tGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGaa                               <  2:1555171/150‑1 (MQ=255)
                                                                                                                    tCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                            >  2:208747/1‑135 (MQ=255)
                                                                                                                    tCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                            <  1:208747/135‑1 (MQ=255)
                                                                                                                     cGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACga                            <  1:1223250/150‑1 (MQ=255)
                                                                                                                      gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGc                                                                <  2:1381335/113‑1 (MQ=255)
                                                                                                                      gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGc                                                                >  1:1381335/1‑113 (MQ=255)
                                                                                                                      gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                            >  1:932271/1‑133 (MQ=255)
                                                                                                                      gTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTgg                                            <  2:932271/133‑1 (MQ=255)
                                                                                                                            cGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCg                     <  2:692944/150‑1 (MQ=255)
                                                                                                                                     gACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTa            <  2:399664/150‑1 (MQ=255)
                                                                                                                                     gACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTa            >  2:938392/1‑150 (MQ=255)
                                                                                                                                         gtgtCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGAc        >  2:1465110/1‑150 (MQ=255)
                                                                                                                                             cGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGcc    <  2:1566689/150‑1 (MQ=255)
                                                                                                                                               aCGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCGTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGCCca  >  2:98203/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGACGGTAAGCCACTGCTCGCTTCTGCTCTACGCGAGGAGGGTAATGGCCCGACAAGCTCGCATGAGATCGCCTAGCAGATCTTGCTAAGGACTGCGTGCCCTATGGGCCCGGCTGTCGTGAACCGAGCCGTCGACGGTGTCGACGTCTCGGCCCTCCGGGCAGCCATCAGTCACGCTTGCGCGCCCCACTTGCCTGCAGCAGAAATTCTGAACATGCTCTGTTCGACCGCTCATGGATTGCTGCGTTTGGGTATGACTCAGAACGAGACAGCGAAGAAGCTAGGACTGCCCA  >  NC_002947/4196200‑4196492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑