Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,264,637 A→G 9.7% S296G (AGC→GGC)  vanA → vanillate O‑demethylase oxygenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,264,6370AG 9.7% 106.5 / 3.5 42S296G (AGC→GGC) vanAvanillate O‑demethylase oxygenase subunit
Reads supporting (aligned to +/- strand):  ref base A (21/16);  new base G (2/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

TCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGATCATCGCCCAAGAGCGTGCGCCGCAGCCG  >  NC_002947/4264515‑4264786
                                                                                                                          |                                                                                                                                                     
ccGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTAGAACGcc                                                                                                                            >  2:108596/2‑150 (MQ=255)
  gACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCcag                                                                                                                          <  1:988733/150‑1 (MQ=255)
  gACATCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGGGCACGACCAGACCCTGACAGACAACATTCGTGAGGGCCGGGGCAAGATTTTCAGCGAAGACCAGGAAATGCTCGAACGCcag                                                                                                                          >  2:516018/1‑150 (MQ=255)
   aCTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCcagc                                                                                                                         >  2:39627/1‑150 (MQ=255)
    cTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCcagca                                                                                                                        >  2:256101/1‑150 (MQ=255)
     ttCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTc                                                                                                                                   >  2:733624/1‑138 (MQ=255)
     ttCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTc                                                                                                                                   <  1:733624/138‑1 (MQ=255)
     ttCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCcagcag                                                                                                                       >  2:731060/1‑150 (MQ=255)
     ttCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCcagcag                                                                                                                       >  2:603883/1‑150 (MQ=255)
             cccTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACct                                                                                                               >  1:839991/1‑150 (MQ=255)
              ccTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACCACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACctg                                                                                                              <  2:839991/150‑1 (MQ=255)
                tGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACctgct                                                                                                            >  2:636233/1‑150 (MQ=255)
                            ctctctctGGTCCTCCTGGGCCATGCCGCGCAATTTGGCTGGGTAGGCGAAGACCTTGCCCGACGAGAGTCGGGAGGGCCAGGGCAAGACTTTCAGCGAATGCCTCGAAATGCCCGAACGCTAGCGGCAGAACCTGCTGGCCCACCACGa                                                                                                <  2:90386/150‑1 (MQ=255)
                            ctctATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCCGCACCGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGa                                                                                                >  1:514907/1‑150 (MQ=255)
                            ctctATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTgg                                                                                                                                           >  2:57274/1‑107 (MQ=255)
                            ctctATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTgg                                                                                                                                           <  1:57274/107‑1 (MQ=255)
                             tctATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAg                                                                                               <  2:5668/150‑1 (MQ=255)
                                aTCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAAt                                                                                                                                       >  1:9482/1‑107 (MQ=255)
                                aTCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAAt                                                                                                                                       <  2:9482/107‑1 (MQ=255)
                                   tGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAAc                                                                                         <  1:256101/150‑1 (MQ=255)
                                    ggTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACctgctg                                                                                                           >  1:740846/1‑131 (MQ=255)
                                    ggTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACctgctg                                                                                                           <  2:740846/131‑1 (MQ=255)
                                    ggTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACt                                                                                        >  1:97921/1‑150 (MQ=255)
                                         ttCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTgctg                                                                                   >  2:1383502/1‑150 (MQ=255)
                                         ttCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTgctg                                                                                   >  1:1633045/1‑150 (MQ=255)
                                                  aTGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCCCAACTTGCTGAAGCTGAat                                                                          >  2:159316/1‑150 (MQ=255)
                                                                    gcgcACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTg                                                        <  2:97921/150‑1 (MQ=255)
                                                                             cAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGc                                               >  1:377305/1‑150 (MQ=255)
                                                                                 cccTGATCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCGGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAg                                           >  2:450897/1‑150 (MQ=255)
                                                                                 cccTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCGGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAg                                           >  2:451018/1‑150 (MQ=255)
                                                                                        cGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCaaa                                            <  2:1243909/142‑1 (MQ=255)
                                                                                        cGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCaaa                                            >  1:1243909/1‑142 (MQ=255)
                                                                                        cGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGa                                    >  2:32497/1‑150 (MQ=255)
                                                                                             acaTTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGa                               <  1:1280598/150‑1 (MQ=255)
                                                                                                 tCGTGAGGGCCAGGGCAAGATTTTCGGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGatcat                           <  1:451018/150‑1 (MQ=255)
                                                                                                 tCGTGAGGGCCAGGGCAAGATTTTCGGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGatcat                           <  1:450897/150‑1 (MQ=255)
                                                                                                  cGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGatcatc                          <  1:731060/150‑1 (MQ=255)
                                                                                                            aGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGATCATCGCCCAAGAGc                <  1:1445412/150‑1 (MQ=255)
                                                                                                                 aaGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTg                                                                                      >  2:1431688/1‑75 (MQ=255)
                                                                                                                 aaGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTg                                                                                      <  1:1431688/75‑1 (MQ=255)
                                                                                                                      tttCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGATCATCGCCCAAGAGCGTGCgccgca      <  1:1383502/150‑1 (MQ=255)
                                                                                                                        tCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGATCATCGCCCAAGAGCGTGCgccgcagc    >  2:105021/1‑150 (MQ=255)
                                                                                                                          aGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGATCATCGCCCAAGAGCGTGCgccgcagccg  >  2:133135/1‑150 (MQ=255)
                                                                                                                          |                                                                                                                                                     
TCGACTTCATCACCCCTGAGAGCGATACCTCTATCTGGTACTTCTGGGGCATGGCGCGCAACTTCGCTGCGCACGACCAGACCCTGACCGACAACATTCGTGAGGGCCAGGGCAAGATTTTCAGCGAAGACCTGGAAATGCTCGAACGCCAGCAGCAGAACCTGCTGGCCCACCCCGAGCGCAACTTGCTGAAGCTGAATATCGACGCCGGCGGCGTGCAGTCACGCAAAGTGCTGGAGCGGATCATCGCCCAAGAGCGTGCGCCGCAGCCG  >  NC_002947/4264515‑4264786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑