Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 352,543 T→C 7.3% L93P (CTG→CCG)  hisF → imidazole glycerol phosphate synthase subunit HisF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947352,5430TC 7.3% 155.0 / 2.6 55L93P (CTG→CCG) hisFimidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base C (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

GCCGACGAAATCACCTTTCTCGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAGGTGAAGCGCC  >  NC_002947/352398‑352690
                                                                                                                                                 |                                                                                                                                                   
gCCGACGAAATCAACTTCCTCGACATCACCGCCAGCGTCGATGGGCGCAACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTc                                                                                                                                                 <  2:1334282/150‑1 (MQ=255)
   gacgaAATCACCTTTCTCGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAAc                                                                                                                                              >  1:1112724/1‑150 (MQ=255)
     cgaAATCACCGTTCTCGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGc                                                                                                                                            <  1:491974/150‑1 (MQ=255)
        aaTCACCTTTCTCGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCgg                                                                                                                                         >  2:1411772/1‑150 (MQ=255)
           cACCTTTCTCGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGc                                                                                                                                      >  1:247578/1‑150 (MQ=255)
               ttgctcGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGAc                                                                                                                                  >  2:682001/4‑150 (MQ=255)
                   tcGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGa                                                                                                                                   <  1:485944/145‑1 (MQ=255)
                   tcGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGa                                                                                                                                   >  2:485944/1‑145 (MQ=255)
                     gACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGtc                                                                                                                            <  1:1242989/150‑1 (MQ=255)
                       cgatgacGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACaa                                                                                                                                <  1:129220/138‑1 (MQ=255)
                       cgatgacGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACaa                                                                                                                                >  2:129220/7‑144 (MQ=255)
                         tCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGa                                                                                                                        <  1:61356/150‑1 (MQ=255)
                          cagcGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCCGt                                                                                                                                        >  2:1642926/4‑133 (MQ=255)
                          cACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGGGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCCGt                                                                                                                                        <  1:1642926/133‑1 (MQ=255)
                            ccGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCa                                                                                                                     >  1:740546/1‑150 (MQ=255)
                                           ggCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGgtgt                                                                                                      <  1:1411772/150‑1 (MQ=255)
                                              gcgACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCa                                                                                                   >  2:1495433/1‑150 (MQ=255)
                                               cgACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCTACGACGGTGCCGACAAGGCCGCGATCAACACCGCCGCGGTGTTCaa                                                                                                  <  2:323175/150‑1 (MQ=255)
                                               cgACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCGGCGGTGttgat                                                                                                  <  1:798100/150‑4 (MQ=255)
                                                  caccacGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAAccc                                                                                               >  2:1198292/1‑150 (MQ=255)
                                                  caccacGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCCGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAAccc                                                                                               >  2:1217186/1‑150 (MQ=255)
                                                     cacGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTTGAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCaga                                                                                            >  1:608594/1‑150 (MQ=255)
                                                         cTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGttt                                                                                        <  1:1198292/150‑1 (MQ=255)
                                                           gCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTTTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGt                                                                                      <  2:746585/150‑1 (MQ=255)
                                                                         gcATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGcggcgg                                                                        >  1:28137/1‑150 (MQ=255)
                                                                          cATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGa                                                                       <  2:1268537/150‑1 (MQ=255)
                                                                           aTGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGAc                                                                      >  2:1569426/1‑150 (MQ=255)
                                                                            tGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTc                                                                                                                                                 <  1:268915/74‑1 (MQ=255)
                                                                            tGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTc                                                                                                                                                 >  2:268915/1‑74 (MQ=255)
                                                                                   ccagGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGGCCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCgg                                                              >  2:939280/1‑150 (MQ=255)
                                                                                       gtgtTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCg                                                          <  2:28137/150‑1 (MQ=255)
                                                                                          ttCATCCCGCTGACGGGTGGGGGTGGCGTGCGGACCGTGCAGGACGTCCGCGACCTGCTCAACGCCGGTGCCGACAAGGTCCCGATCACCACCGCCGCGGTGTGCACCCCAGAGTTTGTCGGCGAGGCGGCGGACCGTTTCGGGGCGCAg                                                       >  2:204869/1‑150 (MQ=255)
                                                                                             aTCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGc                                                    >  1:637534/1‑150 (MQ=255)
                                                                                              tCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCa                                                   >  1:613943/1‑150 (MQ=255)
                                                                                                  gCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGt                                               >  1:1127790/1‑150 (MQ=255)
                                                                                                   cTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGtt                                              <  1:801405/150‑1 (MQ=255)
                                                                                                   cTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCCGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGtt                                              <  1:1217186/150‑1 (MQ=255)
                                                                                                               ggtggCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCACCGCCGGTGCCGACAATGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCTGTTCGCAGTGCATCGTTGTCGCCATCGAt                                  <  2:473154/150‑1 (MQ=255)
                                                                                                                           aCCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAgtg                      >  2:1020688/1‑150 (MQ=255)
                                                                                                                             cGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCcgcg                                                                                                          <  1:656350/64‑1 (MQ=255)
                                                                                                                             cGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCcgcg                                                                                                          >  2:656350/1‑64 (MQ=255)
                                                                                                                             cGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTc                    <  1:939280/150‑1 (MQ=255)
                                                                                                                             cGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTc                    <  1:1020688/150‑1 (MQ=255)
                                                                                                                                    gACATCCGCAACCCGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCaga                                                                                            >  1:329233/1‑71 (MQ=255)
                                                                                                                                    gACATCCGCAACCCGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCaga                                                                                            <  2:329233/71‑1 (MQ=255)
                                                                                                                                     aCATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAg            >  1:1445089/1‑150 (MQ=255)
                                                                                                                                     aCATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGGCGCCATCGATGCCAAGAAAGTGTCCGGGCCAg            <  2:740546/150‑1 (MQ=255)
                                                                                                                                      cATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGAGCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAgg           >  1:1270912/1‑150 (MQ=255)
                                                                                                                                       aTCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAGGt          <  1:1569426/150‑1 (MQ=255)
                                                                                                                                       aTCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAGGt          >  2:523111/1‑150 (MQ=255)
                                                                                                                                          cGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAGGTGaa       <  1:523111/150‑1 (MQ=255)
                                                                                                                                          cGCAACCTGCCCAACGCCGGTGCCGACAAGGCCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAGGTGaa       <  2:247578/150‑1 (MQ=255)
                                                                                                                                           gCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCgg                 <  2:1398076/139‑1 (MQ=255)
                                                                                                                                           gCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCgg                 >  1:1398076/1‑139 (MQ=255)
                                                                                                                                               ccTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGGCGCCATCGATGCCAAGAAAGTGTCCGGGCCAGGTGAAGcgcc  >  1:240450/1‑150 (MQ=255)
                                                                                                                                                 tGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATg                                 >  1:1596317/1‑117 (MQ=255)
                                                                                                                                                 tGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATg                                 <  2:1596317/117‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCGACGAAATCACCTTTCTCGACATCACCGCCAGCGTCGATGGGCGCGACACCACGCTGCATACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACGGTTGGTGGTGGCGTGCGGACCGTGCAGGACATCCGCAACCTGCTCAACGCCGGTGCCGACAAGGTCTCGATCAACACCGCCGCGGTGTTCAACCCAGAGTTTGTCGGTGAGGCGGCGGACCGTTTCGGTTCGCAGTGCATCGTTGTCGCCATCGATGCCAAGAAAGTGTCCGGGCCAGGTGAAGCGCC  >  NC_002947/352398‑352690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑