Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,480,611 T→G 7.7% C146G (TGC→GGC)  PP_3972 → short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,480,6110TG 7.7% 148.3 / 5.0 52C146G (TGC→GGC) PP_3972short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base G (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

TCATCGCACCCTTCGAACACCGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAGGTTCAAGCGCTGG  >  NC_002947/4480462‑4480749
                                                                                                                                                     |                                                                                                                                          
tcatcGCACCCTTCGAACACCGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCt                                                                                                                                            >  1:1240113/1‑150 (MQ=255)
        cccTTCGAACACCGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCCAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCAGGCAAAGgc                                                                                                                                    <  1:459112/150‑1 (MQ=255)
               aaCACCGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCCACGTGGGTGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCt                                                                                                                             >  1:1018727/1‑150 (MQ=255)
               aaCACCGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCt                                                                                                                             >  1:1589589/1‑150 (MQ=255)
                   ccGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGt                                                                                                                         <  1:1408461/150‑1 (MQ=255)
                    cGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCAGGCAAAGgcgtg                                                                                                                                 <  2:137941/141‑3 (MQ=255)
                    cGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCAGGCAAAGgcgtg                                                                                                                                 >  1:137941/1‑139 (MQ=255)
                       agcagcGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTc                                                                                                                     <  1:1042493/150‑1 (MQ=255)
                          agcGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCa                                                                                                                  <  2:481744/150‑1 (MQ=255)
                           gcGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAg                                                                                                                 >  1:163916/1‑150 (MQ=255)
                              gCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGccc                                                                                                              >  1:308080/1‑150 (MQ=255)
                                      cAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCGTCGCACGGCATAGGCCGCGCAACCGCGCTGGCTGGGGCCTGCAAAGTCGCACGCTTGGTGCTCGCAGCCCGCGATgaa                                                                                                      >  1:1600601/1‑150 (MQ=255)
                                      cAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAg                                                                                                                 >  1:162330/1‑139 (MQ=255)
                                      cAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAg                                                                                                                 <  2:162330/139‑1 (MQ=255)
                                       aagaagCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATgaag                                                                                                     >  2:1284780/1‑150 (MQ=255)
                                         gaagCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATgaagaa                                                                                                   >  2:1569671/1‑150 (MQ=255)
                                              cccccGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGc                                                                                                                >  1:1428244/2‑132 (MQ=255)
                                              cccccGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGc                                                                                                                <  2:1428244/131‑1 (MQ=255)
                                                 ccGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCattatt                                                                                           >  1:938457/1‑150 (MQ=255)
                                                     aGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGAc                                                                                       <  1:965879/150‑1 (MQ=255)
                                                               ccGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATg                                                                             >  2:1330705/1‑150 (MQ=255)
                                                                  tCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAAt                                                                          >  1:120411/1‑150 (MQ=255)
                                                                         gCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGa                                                                   >  2:216740/1‑150 (MQ=255)
                                                                           cGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGAtt                                                                 <  2:1053429/150‑1 (MQ=255)
                                                                             cTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGTAGCATTATTTGACGTTCTGGATGAATGCACCGATtgt                                                               <  1:1330705/150‑1 (MQ=255)
                                                                              tGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATtgtg                                                              <  2:120411/150‑1 (MQ=255)
                                                                                      aaaGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGAt                                                      <  2:1084724/150‑1 (MQ=255)
                                                                                      aaaGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGAt                                                      >  2:627429/1‑150 (MQ=255)
                                                                                        aGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGc                                                    <  1:216740/150‑1 (MQ=255)
                                                                                         gTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGAt                                                                                                         >  1:700597/1‑96 (MQ=255)
                                                                                         gTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGAt                                                                                                         <  2:700597/96‑1 (MQ=255)
                                                                                         gTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGcc                                                   <  2:1240113/150‑1 (MQ=255)
                                                                                               gTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCg                                             >  1:916129/1‑150 (MQ=255)
                                                                                               gTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCg                                             <  2:308080/150‑1 (MQ=255)
                                                                                                     acaGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGc                                                    >  1:300471/1‑137 (MQ=255)
                                                                                                     acaGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGc                                                    <  2:300471/137‑1 (MQ=255)
                                                                                                       aGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTcaccac                                     >  2:1221100/1‑150 (MQ=255)
                                                                                                                 aGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTa                                                           >  2:77992/1‑118 (MQ=255)
                                                                                                                 aGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTa                                                           <  1:77992/118‑1 (MQ=255)
                                                                                                                      cATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAg                      >  1:595045/1‑150 (MQ=255)
                                                                                                                       aTAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGCACGGATGCCATAGCGGt                                           >  1:656479/1‑128 (MQ=255)
                                                                                                                       aTAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGCACGGATGCCATAGCGGt                                           <  2:656479/128‑1 (MQ=255)
                                                                                                                             cgcgccgcCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAg               <  2:1018727/150‑1 (MQ=255)
                                                                                                                             cgcgccgcCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAg               <  2:1600601/150‑1 (MQ=255)
                                                                                                                             cgcgccgcCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTAGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAg               >  1:1573471/1‑150 (MQ=255)
                                                                                                                              gcgccgcCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAgg              <  1:1221100/150‑1 (MQ=255)
                                                                                                                                   gccgcGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGCTACGTATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAGGTTCaa         <  1:41563/150‑1 (MQ=255)
                                                                                                                                        gcACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGTTCACCACCGACGTCACCCACAGCGACCAGGTTCAAGCGCt    <  1:1569671/150‑1 (MQ=255)
                                                                                                                                         tgcGCCTTTGCAGGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAGGTTCAAGCGCTg   >  1:148264/3‑150 (MQ=255)
                                                                                                                                          aCGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAGGTTCAAGCGCTgg  <  2:1589589/150‑1 (MQ=255)
                                                                                                                                          aCGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAGGTTCAAGCGCTgg  <  1:1284780/150‑1 (MQ=255)
                                                                                                                                                    cTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATgaagaa                                                                                                   >  1:499264/1‑43 (MQ=255)
                                                                                                                                                    cTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATgaagaa                                                                                                   <  2:499264/43‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                          
TCATCGCACCCTTCGAACACCGTAGCAGCGGCACAGAGCAAGAAGCTCCCCGTAGGGGATATCCCGTCAGGGGGCCGCTGCGTGGCAAAGTTGTGGTAATCACAGGGGCATCCAGCGGCATAGGCCGCGCCGCCGCGCACGCCTTTGCCTGCAAAGGCGCACGCTTGGTGCTCGCAGCCCGCGATGAAGAAGCATTATTTGACGTTCTGGATGAATGCACCGATTGTGGTACGGATGCCATAGCGGTCACCACCGACGTCACCCACAGCGACCAGGTTCAAGCGCTGG  >  NC_002947/4480462‑4480749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑